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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 112 (1994), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Twelve fertile alloplasmic wheat lines, produced by substituting four nuclear genomes of Triticum aestivum into the cytoplasms of T. timococcum Kostoff, T. timopheevi Zhuk., and Aegilops ventricosa Tausch, were examined for cytoplasmic effects on falling number and α-amylase activity during grain development in different environments. Nuclear genome, cytoplasm and environment were important sources of variation for falling number. In one environment, where the plants were exposed to very hot, dry conditions, the levels and patterns of change in α-amylase activity were also significantly affected by the cytoplasm. The data indicated that alien wheat cytoplasms might result in useful additional variation in specific environments where sprouting is a problem.
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  • 2
    ISSN: 1432-0983
    Keywords: Key words Triticale ; Mitochondrial DNA ; orf25 gene ; Transcriptional expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Triticale, an intergeneric hybrid crop-plant, is generated when female wheat lines are fertilised with pollen from rye. We have investigated the mitochondrial DNA organisation and the expression of a total of 11 different triticale genotypes, varying in their nuclear and cytoplasmic backgrounds. In Southern hybridisations using probes homologous to the upstream flanking sequences, mtDNA fragments characteristic of both wheat and rye mtDNA can be detected in all triticale lines analysed. In addition, clones isolated fom a triticale lambda library exhibit either a maternal-like or paternal-like organisation of the orf25 gene region. By PCR cloning, four different orf25 gene copies were identified in triticale, three of which correspond to maternal (85%) or paternal (12%) orf25 sequences. Three percent of all clones represent a novel type, that might have arisen by homologous recombination. Although these data suggest biparental inheritance of mtDNA in wheat/rye crosses, paternal-like gene copies can also be detected in maternal wheat mitochondria. Their stoichiometry as assayed by competitive PCR is about 0.1% of total orf25 gene copies. The high abundance of paternal-like sequences in the F1 hybrid might therefore be due to either the transmission of rye mtDNA in the intergeneric cross and/or the amplification of sequences in triticale that persist in sub-stoichiometric amounts in wheat. These data suggest that amplification and recombination of sub-genomic mitochondrial molecules are affected by different nuclear genotypes. Interestingly, sequence analysis of triticale RT-PCR clones indicates a selective transcription of maternal-like orf25 gene copies in triticale. Mitochondrial gene expression may therefore possess mechanisms to compensate for the variation of mtDNA organisation.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 32 (1983), S. 593-600 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Triticum durum ; durum wheat ; Secale cereale ; inbred lines ; intergeneric crossability ; embryo development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Crossability and embryo development were studied in the crosses of one Triticum aestivum and three T. durum genotypes with nineteen rye inbred lines. Parental wheat and rye genotypes exerted a significant influence on the characters seed set, number of seeds containing embryos and viable plantlets obtained from embryo culture. It was established that the common winter wheat cultivar Götz is of intermediate crossability. The rye inbred lines varied substantially in their capacity to fertilize several wheat genotypes. Interactions between wheats of different crossability classes and their seed set with rye lines were detected. Significant correlations were obtained between seed set and viable plantlets recovered in vitro.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 33 (1984), S. 233-239 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; read wheat ; Triticum durum ; durum wheat ; Secale cereale ; rye ; inbred lines ; intergeneric crossability ; embryo differentiation ; plantlet recovery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary One hexaploid wheat cultivar (Triticum aestivem) and two tetraploid wheat lines (T. durum) were crossed with seventeen inbred lines of rye (Secale cereale). Seed set, degree of hybrid embryo differentiation at the time of excision for in vitro culture and recovery of amphihaploid plantlets from various embryo categories were studied. Degree of embryo differentiation was predominantly determined by maternal wheats, paternal rye genotypes appearing to be of minor importance. T. aestivum x rye hybrid embryos were superior to those produced from T. durum for degree of differentiation. The proportion of plantlets developing from differentiated embryos was high for all wheat parents, whereas undifferentiated embryos were mostly unsuitable for plantlet production. The results revealed that cross-incompatibility in hexaploid wheat x rye crosses was due to failure of fertilization, while in tetraploid wheat x rye crosses it was caused by lack of embryo differentiation. Correlation analyses showed that seed set provided a criterion to predict the amphihaploid plantlets to be expected from a particular wheat x rye combination.
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  • 5
    ISSN: 1573-5060
    Keywords: deoxynivalenol ; Fusarium graminearum isolates ; genetic parameters ; head blight resistance ; triticale ; Triticosecale
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fusarium head blight infection causes severe yield losses and contamination of the grain with mycotoxins in triticale (× Triticosecale Wittmack) grown in temperate and semihumid areas. In a two-year experiment thirty-six genotypes were inoculated separately with two isolates of Fusarium graminearum differing fivefold in their in vitro deoxynivalenol (DON) production and the effect on various traits was studied. All traits were significantly affected by head blight. The two isolates differed considerably in their aggressiveness resulting in a mean reduction of grain weight per spike of almost 25% and 50%, respectively. Inter-annual correlation was high for average disease rating (r=0.63, P〈-0.01) and low for the other traits. Therefore, disease rating, averaged from two to three records, was regarded a suitable criterion for screening purposes. The effect of isolates on genotypes was not stable over years. The mean DON content of five genotypes with diverse resistance levels was 68 mg kg-1. In vitro DON production of the two isolates used for inoculation did not correspond to their aggressiveness and DON contamination of the grain.
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  • 6
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