Publication Date:
2018
Description:
l‐Galactose (l‐Gal) is one of the components of plant cell wall polysaccharides. In the GDP‐l‐fucose‐deficient Arabidopsis thaliana mutant mur1, l‐fucose (l‐Fuc) residues in xyloglucan are substituted by l‐Gal residues. l‐Gal only differs from l‐Fuc by the presence of an oxygen at C‐6. Thus, we hypothesized that the A. thaliana xyloglucan α1,2‐l‐fucosyltransferase (AtFUT1) is also responsible for the l‐galactosyl transfer to d‐galactose residues in xyloglucan. In this study, we heterologously produced AtFUT1 in fission yeast and carried out an in vitro assay for the activities of AtFUT1 on GDP‐l‐Gal and xyloglucan oligosaccharide. We show that the recombinant AtFUT1 catalyzes l‐Gal transfer to xyloglucan oligosaccharides although the initial velocity of l‐Gal transfer is 3.1 times lower than that of l‐Fuc transfer.
Print ISSN:
0014-5793
Electronic ISSN:
1873-3468
Topics:
Biology
,
Chemistry and Pharmacology
Permalink