Publication Date:
2012-04-14
Description:
The Drosophila dorsal-ventral (DV) axis is polarized when the oocyte nucleus migrates from the posterior to the anterior margin of the oocyte. Prior work suggested that dynein pulls the nucleus to the anterior side along a polarized microtubule cytoskeleton, but this mechanism has not been tested. By imaging live oocytes, we find that the nucleus migrates with a posterior indentation that correlates with its direction of movement. Furthermore, both nuclear movement and the indentation depend on microtubule polymerization from centrosomes behind the nucleus. Thus, the nucleus is not pulled to the anterior but is pushed by the force exerted by growing microtubules. Nuclear migration and DV axis formation therefore depend on centrosome positioning early in oogenesis and are independent of anterior-posterior axis formation.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459055/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459055/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zhao, Tongtong -- Graham, Owen S -- Raposo, Alexandre -- St Johnston, Daniel -- 080007/Wellcome Trust/United Kingdom -- 092096/Wellcome Trust/United Kingdom -- A14492/Cancer Research UK/United Kingdom -- Cancer Research UK/United Kingdom -- Wellcome Trust/United Kingdom -- New York, N.Y. -- Science. 2012 May 25;336(6084):999-1003. doi: 10.1126/science.1219147. Epub 2012 Apr 12.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉The Gurdon Institute and the Department of Genetics, University of Cambridge, Cambridge, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22499806" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Body Patterning
;
Cell Nucleus/*physiology/ultrastructure
;
Cell Polarity
;
Centrosome/physiology
;
Drosophila
;
Drosophila Proteins/physiology
;
Dyneins/physiology
;
Microtubule-Organizing Center/physiology/ultrastructure
;
Microtubules/*physiology/ultrastructure
;
Movement
;
Mutation
;
Nuclear Envelope/physiology/ultrastructure
;
Oocytes/*physiology/ultrastructure
;
*Oogenesis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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