Publication Date:
2002-11-09
Description:
Electron tomography of vitrified cells is a noninvasive three-dimensional imaging technique that opens up new vistas for exploring the supramolecular organization of the cytoplasm. We applied this technique to Dictyostelium cells, focusing on the actin cytoskeleton. In actin networks reconstructed without prior removal of membranes or extraction of soluble proteins, the cross-linking of individual microfilaments, their branching angles, and membrane attachment sites can be analyzed. At a resolution of 5 to 6 nanometers, single macromolecules with distinct shapes, such as the 26S proteasome, can be identified in an unperturbed cellular environment.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Medalia, Ohad -- Weber, Igor -- Frangakis, Achilleas S -- Nicastro, Daniela -- Gerisch, Gunther -- Baumeister, Wolfgang -- New York, N.Y. -- Science. 2002 Nov 8;298(5596):1209-13.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Max Planck Institute for Biochemistry, D-82152 Martinsried, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12424373" target="_blank"〉PubMed〈/a〉
Keywords:
Actin Cytoskeleton/chemistry/metabolism/*ultrastructure
;
Actins/ultrastructure
;
Animals
;
Binding Sites
;
Cell Membrane/metabolism/ultrastructure
;
Cell Movement
;
Dictyostelium/chemistry/physiology/*ultrastructure
;
Endoplasmic Reticulum, Rough/ultrastructure
;
Freezing
;
*Image Processing, Computer-Assisted
;
Macromolecular Substances
;
Microfilament Proteins/*ultrastructure
;
Organelles/*ultrastructure
;
Peptide Hydrolases/ultrastructure
;
*Proteasome Endopeptidase Complex
;
Proteome
;
Protozoan Proteins/ultrastructure
;
Ribosomes/ultrastructure
;
Tomography/*methods
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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