ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

1

Electronic Resource

Development of alginate gel dressing (1998)

Suzuki, Yoshihisa ; Nishimura, Yoshihiko ; Tanihara, Masao ; [et al.]

Springer

Journal of artificial organs 1 (1998), S. 28-32

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ISSN: 1619-0904

Keywords: Alginate ; Wound dressing ; Wound healing Skin graft

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine , Technology

Notes: Abstract Alginate-based wound dressing materials have been widely used to promote wound healing and to reduce blood loss from wounds. However, recently a few drawbacks of well-established commercial alginate dressings have been reported. Therefore, we tried to develop a new alginate dressing to reduce the drawbacks. First, four new dressings with different calcium content were prepared, and the cytotoxicity of these four materials, and Kaltostat and Sorbsan, was tested in vitro by culture of fibroblasts with their extracts. Second, full-thickness wounds in pigs were used for the evaluation of wound healing in vivo. Finally, a newly developed alginate dressing was used clinically for treatment of split-thickness skin graft donor sites. The extract medium from ALG3, ALG4, Kaltostat, and Sorbsan induced a significant inhibitory effect on proliferation of fibroblasts. As for wound closure rate, the ALG2-covered wounds had the smallest wound area on day 15. Histologically, foreign-body reaction was least in ALG2-treated wounds. In a clinical study, the main drawback of ALG2 was leakage of wound exudate due to dissolution of the dressing material. However, the transparency of moistened ALG2 allowed easy evaluation of the wound, and after healing it was easy to remove ALG2 from the wound without injury to the reepithelialized skin because ALG2 was relatively nonadherent to the wound.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01340449

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2

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Evaluation of collagen nerve guide in facial nerve regeneration (1998)

Kitahara, Américo K. ; Suzuki, Yoshihisa ; Nishimura, Yoshihiko ; [et al.]

Springer

Journal of artificial organs 1 (1998), S. 22-27

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ISSN: 1619-0904

Keywords: Collagen ; Facial nerve ; Nerve paralysis ; Nerve conduit ; Nerve regeneration

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine , Technology

Notes: Abstract Facial nerve paralysis due to resection of tumors or as a consequence of trauma is a frequently observed complication. Thus, in the present study, we evaluated a collagen nerve guide in facial nerve regeneration across a 5-mm nerve gap. This biological tube was manufactured from 3% collagen, coated over a Teflon tube used only as a template and submitted to thermal dehydration at 105°C for 24h. The collagen tube was implanted at the dorsal ramous of the facial nerve of five adult cats over a gap of 5mm. The facial nerve of the contralateral side was kept intact and used as control. Electrophysiological study was performed from 3 weeks after surgery, and histological and horseradish peroxidase labeling examination was carried out 8 weeks after implantation. Electrophysiological study confirmed the recovery of electrical activity of the collagenimplanted regenerated nerve. Light-microscopic examination of collagen tube-implanted specimens revealed a well vascularized regenerated nerve, which under an electron microscope showed many myelinated axons surrounded by Schwann cells and unmyelinated axons. Horseradish peroxidase staining demonstrated labeling of facial motoneurons in the brainstem and facial nerve terminals in the neuromuscular junction, also confirming restoration of the whole facial nerve tract from the reinnervated muscles, passing through the regenerated site to the brainstem. The collagen tube was very efficient as a nerve guide over a 5mm facial nerve gap and shows great promise as a nerve conduit.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01340448

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3

Electronic Resource

A new drug delivery system with controlled release of antibiotic only in the presence of infection (1998)

Suzuki, Yoshihisa ; Tanihara, Masao ; Nishimura, Yoshihiko ; [et al.]

Hoboken, NJ : Wiley-Blackwell

Journal of Biomedical Materials Research 42 (1998), S. 112-116

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ISSN: 0021-9304

Keywords: drug delivery system ; occlusive dressing ; antibiotic ; drug-polymer conjugate ; infection ; Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Medicine , Technology

Notes: An ideal drug delivery system (DDS) releases an appropriate drug at specific locations and times. We tried to create a new antibiotic delivery system that releases gentamicin only when wounds are infected by Pseudomonas aeruginosa (P.A.). Exudate from the dorsal pouch of rats infected with P.A. showed significantly higher hydrolytic activity - thrombin-like activity - toward Boc-Val-Pro-Arg-MCA than exudate from noninfected wounds. We therefore constructed a device for controlled release of an antimicrobial drug triggered by thrombin-like activity. Briefly, gentamicin was bound to a polyvinyl alcohol derivative (PVA) hydrogel through a newly developed peptide linker cleavable by the proteinase, PVA-(linker)-gentamicin. In vitro experiments showed that proteinases from wounds infected with P.A. cleaved the linker and gentamicin was released while the exudate from noninfected wounds had no hydrolytic activity toward the linker. This device shows potential as an occlusive dressing with an effective antibiotic delivery system for treating infected wounds. © 1998 John Wiley & Sons, Inc. J. Biomed Mater Res, 42, 112-116, 1998.

Additional Material: 3 Ill.

Type of Medium: Electronic Resource

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4

Electronic Resource

Evaluation of a novel alginate gel dressing: Cytotoxicity to fibroblasts in vitro and foreign-body reaction in pig skin in vivo (1998)

Suzuki, Yoshihisa ; Nishimura, Yoshihiko ; Tanihara, Masao ; [et al.]

Hoboken, NJ : Wiley-Blackwell

Journal of Biomedical Materials Research 39 (1998), S. 317-322

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ISSN: 0021-9304

Keywords: calcium alginate dressing ; wound healing ; foreign body ; occlusive dressing ; artificial skin ; Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Medicine , Technology

Notes: Calcium alginate dressings have beneficial effects on wound healing by providing a moist wound environment. However, cytotoxicity and the nonbiodegradable nature of calcium alginate dressings induce unresolved chronic foreign-body reaction. In this study, a novel freeze-dried alginate gel dressing (AGA-100) low in calcium ions was evaluated for cytotoxicity to L929 cells in vitro and in full-thickness pig wounds in vivo. Cytotoxicity testing on L929 cells showed the cytocompatibility of AGA-100 extracts, while extracts from Kaltostat, a well-established alginate dressing, induced cytopathic effects. In an in vivo study using pigskin, AGA-100, Kaltostat, and gauze were applied on 1-in-diameter circular full-thickness wounds on the back of pigs and the time course of wound closure was evaluated. Kaltostat and gauze dressings were used as controls. For histologic evaluation, wound tissue was harvested on day 18. AGA-100-treated wounds showed rapid wound closure compared to control wounds on day 15. Foreign-body reaction was marked in Kaltostat- and gauze-treated wounds, and differed significantly from AGA-100-treated wounds. Based on these data, AGA-100 could reduce the cytotoxicity to fibroblasts and foreign-body reaction that have been observed with currently available calcium alginate dressings; it was also found to be useful as an alginate dressing. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 39, 317-322, 1998.

Additional Material: 5 Ill.

Type of Medium: Electronic Resource

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5

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Parallel re-modeling of EF-1alpha function: divergent EF-1alpha genes co-occur with EFL genes in diverse distantly related eukaryotes (2013)

Ryoma Kamikawa; Matthew Brown; Yuki Nishimura; Yoshihiko Sako; Aaron Heiss; Naoji Yubuki; Ryan Gawryluk; Alastair Simpson; Andrew Roger; Tetsuo Hashimoto; Yuji Inagaki

BioMed Central

In: BMC Evolutionary Biology

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Publication Date: 2013-06-27

Description: Background: Elongation factor-1alpha (EF-1alpha) and elongation factor-like (EFL) proteins are functionally homologous to one another, and are core components of the eukaryotic translation machinery. The patchy distribution of the two elongation factor types across global eukaryotic phylogeny is suggestive of a 'differential loss' hypothesis that assumes that EF-1alpha and EFL were present in the most recent common ancestor of eukaryotes followed by independent differential losses of one of the two factors in the descendant lineages. To date, however, just one diatom and one fungus have been found to have both EF-1alpha and EFL (dual-EF-containing species). Results: In this study, we characterized 35 new EF-1alpha/EFL sequences from phylogenetically diverse eukaryotes. In so doing we identified 11 previously unreported dual-EF-containing species from diverse eukaryote groups including the Stramenopiles, Apusomonadida, Goniomonadida, and Fungi. Phylogenetic analyses suggested vertical inheritance of both genes in each of the dual-EF lineages. In the dual-EF-containing species we identified, the EF-1alpha genes appeared to be highly divergent in sequence and suppressed at the transcriptional level compared to the co-occurring EFL genes. Conclusions: According to the known EF-1alpha/EFL distribution, the differential loss process should have occurred independently in diverse eukaryotic lineages, and more dual-EF-containing species remain unidentified. We predict that dual-EF-containing species retain the divergent EF-1alpha homologues only for a sub-set of the original functions. As the dual-EF-containing species are distantly related to each other, we propose that independent re-modelling of EF-1alpha function took place in multiple branches in the tree of eukaryotes.

Electronic ISSN: 1471-2148

Topics: Biology

Published by BioMed Central

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Accelerated tissue regeneration through incorporation of basic fibroblast growth factor-impregnated gelatin microspheres into artificial dermis (2000)

Kawai, Katsuya ; Suzuki, Shigehiko ; Tabata, Yasuhiko ; [et al.]

Elsevier

In: Biomaterials. 2000; 21(5): 489-499. Published 2000 Mar 01. doi: 10.1016/s0142-9612(99)00207-0.

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Publication Date: 2000-03-01

Print ISSN: 0142-9612

Electronic ISSN: 1878-5905

Topics: Biology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Medicine