ISSN:
1573-501X
Keywords:
block shuffling
;
combinatorial chemistry
;
end-to-end distance
;
evolutionary molecular engineering
;
T4 RNA ligase
;
Y-ligation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Very efficient ligation ofoligodeoxyribonucleotides was attained through asimple molecular construct, which is composed of onestem and two branches (Y-shape), with use of T4 RNAligase. Single-stranded DNAs (naturally, RNAs also) ofmore than 100 nucleotides (even 800 nts) wereconsiderably ligated, approximately as theoreticallyexpected. Owing to the molecular construct adopted,such a tiny amount of ligation products could beamplified to a sufficient amount by PCR and thenrecovered as single-stranded DNAs. This advantage ofbeing amplifiable is shown to be useful for bothcombinatorial chemistry and evolutionary molecularengineering, which deal with a pool of diversity molecules.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1009644028931
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