ISSN:
1573-5044
Keywords:
androgenesis
;
coconut milk
;
Coffea arabica
;
microspore culture
;
microspore division
;
multicellular microspores
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The number of dividing microspores of Coffea arabica L. cv. Catuai and Catimor could be drastically increased in microspore media containing 16% (w/v) coconut milk, allowing cell divisions to continue in the microspore and multicellular microspores to survive until day 60. After a cold treatment, the microspores were mechanically isolated prior to cultivation in Murashige and Skoog medium supplemented with sucrose and maltose and (mg l-1) 2,4-d: 2, BAP: 1 or a combination of kinetin: .5, 2,4-d: .5 and NAA: .5 as stationary suspension at a density of 1,200/ml. The crucial stage during microsporogenesis suitable for in vitro androgenesis proved to be mid uninucleate till early binucleate in flowerbuds with the size of 13–15 mm two to three days before anthesis. The initial steps of androgenesis were determined.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00041118
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