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  • 1
    Electronic Resource
    Electronic Resource
    Regeneration of Pea (Pisum sativum L.) Plantlets by in vitro Culture of Immature Embryos (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 99 (1987), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Plant regeneration was achieved from immature embryo-derived, calli of Pisum sativum. Embryo axes were separated from cotyledons and cultured on different media containing BAP and NAA until plantlet regeneration. Rooting of the plantlets was obtained on MS medium supplemented with 2 mg/1 IBA. Frequency of regeneration was shown to be under the influence of the genotype. Histological preparations showed de novo origin of the shoots via organogenesis. Out of 2C regenerated plantlets, 11 were diploids (2n = 14) arid 9 aneusomatic (chromosomal mosaics) with chromosome numbers ranging from 12 to 16.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1987.tb01168.x
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  • 2
    Electronic Resource
    Electronic Resource
    Grafting in vitro Regenerated Pea (Pisum sativum L.) Shoots as a Method for Obtaining Mature Plants (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 102 (1989), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A method of grafting pea plants is proposed to allow rapid availability of a large number of mature plants from in vitro regenerated shoots.Plant regeneration was obtained from macerated vegetative apices and immature embryos, but regenerated shoots rooted with low frequency (ca. 10 %). When those shoots were grafted onto seedling stocks of the same cultivar, 80 to 85 % of the grafts survived, grew to maturity and produced seed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1989.tb01267.x
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  • 3
    Electronic Resource
    Electronic Resource
    Nuclear DNA changes within Helianthus annuus L.
    Amsterdam : Elsevier
    Cell Biology International Reports 14 (1990), S. 80 
    Details
    ISSN: 0309-1651
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0309-1651(90)90422-U
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  • 4
    Electronic Resource
    Electronic Resource
    Amplification of nuclear DNA sequences during induced plant cell dedifferentiation
    Amsterdam : Elsevier
    Cell Differentiation 18 (1986), S. 157-161 
    Details
    ISSN: 0045-6039
    Keywords: DNA amplification ; Vicia faba ; cell differentiation
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0045-6039(86)90081-3
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  • 5
    Electronic Resource
    Electronic Resource
    In vitro culture of Aloe barbadensis Mill.: quantitative DNA variations in regenerated plants
    Amsterdam : Elsevier
    Plant Science 91 (1993), S. 223-229 
    Details
    ISSN: 0168-9452
    Keywords: Aloe barbadensis Mill. ; basic DNA content variation ; cell dimensions ; cytophotometry ; heterochromatin ; regenerated plants
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(93)90145-P
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  • 6
    Electronic Resource
    Electronic Resource
    In vitro culture of Aloe barbadensis Mill.: Morphogenetic ability and nuclear DNA content
    Amsterdam : Elsevier
    Plant Science 55 (1988), S. 53-59 
    Details
    ISSN: 0168-9452
    Keywords: Aloe barbadensis ; callus ; chromosome endoreduplication ; cytophotometry ; nuclear DNA content ; plant regeneration
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(88)90041-6
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  • 7
    Electronic Resource
    Electronic Resource
    Cytological analyses of in vitro somatic embryogenesis in Brimeura amethystina salisb. (Liliaceae)
    Amsterdam : Elsevier
    Plant Science 62 (1989), S. 255-261 
    Details
    ISSN: 0168-9452
    Keywords: Brimeura amethystina ; cytophotometry ; somatic embryogenesis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(89)90088-5
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  • 8
    Electronic Resource
    Electronic Resource
    Nuclear DNA changes within Helianthus annuus L.: cytophotometric, karyological and biochemical analyses (1986)
    Springer
    Theoretical and applied genetics 73 (1986), S. 20-26 
    Details
    ISSN: 1432-2242
    Keywords: Helianthus annuus ; Intraspecific DNA changes ; Cytophotometry ; Karyology ; Chemicophysical DNA characterization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cytophotometric measurement of the root meristems of seedlings after Feulgen-staining reveals that large differences (up to 58.16%) in nuclear DNA content may occur in the thirty-one cultivated varieties or lines of Helianthus annuus tested. Significant variations (not exceeding 25%) in the amount of DNA, which does not differ between the root and the shoot meristems of a single seedling, are also found to exist within cultivars or lines; even seedlings obtained from seeds collected from different portions of single heads of plants belonging to a selfed line may vary one from the other in this respect. Variations in the number of chromosomes or alterations in the chromosome structure do not account for the differences observed in nuclear DNA content. Karyometric analyses demonstrate that the surface area of squashed interphase nuclei and metaphase chromosomes and the total length of the latter increase with the increase in Feulgen/DNA absorption. DNA thermal denaturation and reassociation kinetics indicate that a frequency variation in repeated DNA sequences goes hand in hand with changes in the size of the genome. These results, supporting the concept that a plant genome is highly flexible, are discussed in relation to other data to be found in the literature on the intraspecific variation in the nuclear DNA content and in relation to the way in which it is produced in H. annuus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273713
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  • 9
    Electronic Resource
    Electronic Resource
    Nuclear DNA changes within Helianthus annuus L.: variations in the amount and methylation of repetitive DNA within homozygous progenies (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 285-291 
    Details
    ISSN: 1432-2242
    Keywords: Helianthus annuus ; Intraspecific DNA changes ; Repetitive DNA ; DNA methylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Complex alterations in the redundancy and methylation of repeated DNA sequences were shown to differentiate the nuclear genome of individuals belonging to single progenies of homozygous plants of the sunflower. DNA was extracted from seedlings obtained from seeds collected at the periphery of flowering heads (P DNA) or from seedlings obtained from seeds collected in their middle (M DNA). Three fractions of repeated sequences were isolated from genomic DNA: a highly repetitive fraction (HR), which reassociates within an equivalent Cot of about 2 × 10-1, and two medium repetitive fractions (MR1 and MR2) having Cot ranges of about 2 × 10-1-2 and 2-102, respectively. Denaturation kinetics allowed different sequence families to be recognized within each fraction of repetitive DNA, and showed significant differences in sequence redundancy to occur between P and M DNA, particularly as far as the MR2 fraction is concerned. Most DNA sequence families are more represented in P DNA than in M DNA. However, the redundancy of certain sequences is greater in the latter than in the former. Each repetitive DNA fraction was hybridized to Southern blots of genomic P or M DNA which was digested to completion by three pairs of isoschizomeric restriction endonucleases which are either insensitive or sensitive to the methylation of a cytosine in the recognition site. The results obtained showed that the repetitive DNA of H. annuus is highly methylated. Clear-cut differences in the degree of methylation of P and M DNA were found, and these differences were particularly apparent in the MR2 fraction. It is suggested that alterations in the redundancy of given DNA sequences and changes in their methylation patterns are complementary ways to produce continuous genotypic variability within the species which can be exploited in environmental adaptation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223670
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  • 10
    Electronic Resource
    Electronic Resource
    Nuclear DNA changes within Helianthus annuus L.: variations in the amount and methylation of repetitive DNA within homozygous progenies (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 285-291 
    Details
    ISSN: 1432-2242
    Keywords: Key words Helianthus annuus  ;  Intraspecific DNA changes  ;  Repetitive DNA  ;  DNA methylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Complex alterations in the redundancy and methylation of repeated DNA sequences were shown to differentiate the nuclear genome of individuals belonging to single progenies of homozygous plants of the sunflower. DNA was extracted from seedlings obtained from seeds collected at the periphery of flowering heads (P DNA) or from seedlings obtained from seeds collected in their middle (M DNA). Three fractions of repeated sequences were isolated from genomic DNA: a highly repetitive fraction (HR), which reassociates within an equivalent Cot of about and two medium repetitive fractions (MR1 and MR2) having Cot ranges of about and respectively. Denaturation kinetics allowed different sequence families to be recognized within each fraction of repetitive DNA, and showed significant differences in sequence redundancy to occur between P and M DNA, particularly as far as the MR2 fraction is concerned. Most DNA sequence families are more represented in P DNA than in M DNA. However, the redundancy of certain sequences is greater in the latter than in the former. Each repetitive DNA fraction was hybridized to Southern blots of genomic P or M DNA which was digested to completion by three pairs of isoschizomeric restriction endonucleases which are either insensitive or sensitive to the methylation of a cytosine in the recognition site. The results obtained showed that the repetitive DNA of H. annuus is highly methylated. Clear-cut differences in the degree of methylation of P and M DNA were found, and these differences were particularly apparent in the MR2 fraction. It is suggested that alterations in the redundancy of given DNA sequences and changes in their methylation patterns are complementary ways to produce continuous genotypic variability within the species which can be exploited in environmental adaptation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050126
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