ISSN:
1618-2650
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract 31P-NMR chemical shifts and coupling constants of nine inorganic phosphorus compounds composed of different structural units or oxidation numbers PV, PIV, PIII, and PI were measured in the pH-range 3 ∼ 11. A concise map of NMR data providing the pH-dependence of the chemical shift (δ-pH map) was set up to be used for identifying phosphorus compounds under varying pH-conditions. Chemical shifts of monofluorophosphate, as well as most phosphorus compounds of oxidation numbers 5 and 4, were greatly dependent on pH, in contrast to the less or negligible pH-dependence of phosphorus compounds of oxidation numbers 1 and 3. Monofluorophosphate gave the parameters: δ=+1.3±0.2 ppm and 1JPF=870±0.2 Hz, that remained unchanged at pH〉6, but varied at pH〈6. The practical use of the δ-pH map was shown with a few kinetic experiments in which monofluorophosphate was enzymatically hydrolyzed by alkaline phosphatase (EC3.1.3.1) at pH 7.2 and non-enzymatically at pH 3.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00325563
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