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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 146 (1997), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A strain of Bullera sinensis that secretes a fungicidal thermolabile and protease-sensitive toxin was found. Its killer phenotype was cureless. Ascomycetous, ustilaginaceous yeasts and the members of the Sporidiales are insensitive to the B. sinensis killer toxin. The mycocin acts against tremellaceous yeasts only, except for Cystofilobasidium, Fellomyces and Trichosporon spp. The genera Mrakia, Bullera, Cryptococcus and Udeniomyces are heterogeneous in sensitivity pattern. The different responses of some anamorphic species to this mycocin are often consistent with the origin of the strains and the differences in sexual, chemotaxonomic and physiological characteristics between them.
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  • 2
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Yeasts of various genera were screened for denitrifying activity, and several yeast strains such as Trichosporon cutaneum, Fellomyces fuzhouensis, and Candida sp. were found to exhibit distinct activities to convert nitrite to nitrous oxide. Dissimilatory nitrite reductase (Nir) or nitric oxide reductase (Nor) activities were detected in the cell-free extracts of these yeasts. Spectrophotometric as well as Western blot analyses showed that T. cutaneum contains Nor of the cytochrome P450 type. This is the first report that shows that denitrification is also distributed among yeasts although their systems are incomplete, only capable of reducing nitrite to nitrous oxide.
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  • 3
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A new ballistosporous anamorphic yeast genus Udeniomyces is proposed for three Bullera species forming large bilaterally symmetrical ballistospores. This genus is characterized by large bilaterally symmetrical ballistospores, the presence of xylose in the cells, Q-10 as the major ubiquinone, the presence of budding yeast cells and the lack of stalked conidia. Three new combinations, Udeniomyces puniceus (Komagata et Nakase) Nakase et Takematsu comb. nov., Udeniomyces piricola (Stadelmann) Nakase et Takematsu comb. nov. and Udeniomyces megalosporus (Nakase et Suzuki) Nakase et Takematsu comb. nov. are proposed. Udeniomyces piricola is designated as the type species of the genus.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 170 (1998), S. 155-161 
    ISSN: 1432-072X
    Keywords: Key words Archaea ; Hyperthermophile ; Thermoproteus ; Intron ; 16S rRNA gene ; LAGLI-DADG-like motif
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Multiple introns were detected in the 16S rRNA gene of newly isolated Thermoproteus species strains IC-033 and IC-061 and Thermoproteus neutrophilus JCM 9278. In the 16S rRNA gene of strain IC-033, five introns of 627, 762, 636, 33, and 682 bp existed after positions 548, 781, 1092, 1205, and 1213 (according to the Escherichia coli numbering system), respectively. Likewise, strain IC-061 possessed 764-, 32-, and 688-bp introns after positions 781, 1205, and 1213, respectively; and T. neutrophilus JCM 9278 had 34- and 663-bp introns after positions 1205 and 1213, respectively. All the introns carried the putative intron core structures consisting of a bulge-helix-bulge motif and a long stable stem. The large introns carried open reading frames containing the LAGLI-DADG-like motifs in their terminal inserts; however, three out of four large introns of strain IC-033 seemed to incur frameshift mutations. Occurrence of introns at the same insertion sites in the three strains would allow tracing of the evolutionary movements of these introns.
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  • 5
    ISSN: 1618-2545
    Keywords: Aphyllophorales ; ribosomalDNA ; synnematous hyphomycete ; taxonomy ; Tretopileus sphaerophorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tretopileus sphaerophorus, a synnematous hyphomycete with basidiomycetous affinities was newly isolated from the decaying petiole and peduncle ofCocos nucifera collected in Depok, Indonesia. The species produced first a bulbil as a propagule on the top of a synnema. After the bulbil had fallen, the synnema proliferated about seven times to produce new bulbils, each time making conspicuous nodes at the upper part. By careful morphological observation, clamp connections were confirmed on the hyphae in the specimens and culture. In culture, each hyphal cell with or without a clamp was found to be dikaryotic by DAPI nuclear staining. Germination of the bulbils occurred first from projecting hyphal tips on their upper surface, which have been treated as germ pores. The inner structure of the bulbils, the hyaline mucus of the bulbils, and conidium-like hyphal fragments were also examined. Phylogenetically,T. sphaerophorus was inferred to be related to the Aphyllophorales based on the nuclear encoded small subunit (18S) rDNA using the homology search system (FASTA) and the neighbour-joining method.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Mycoscience 38 (1997), S. 25-29 
    ISSN: 1618-2545
    Keywords: Bullera alba ; killer toxin ; microcin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A strain ofBullera alba that secretes a killer toxin inhibitory (at pH values ranging from 3 to 7) to many ascomycetous and basidiomycetous yeast-like fungi was discovered. Its killer phenotype was incurable. The toxin was relatively thermostable and resistant to many proteases, and it was identified as a microcin. It inhibited the growth of some pathogenic yeasts and was the most active againstCryptococcus neoformans.
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  • 7
    ISSN: 1618-2545
    Keywords: phylogeny ; SSU rDNA ; Trichosporon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Small subunit ribosomal DNA sequences of all species of the basidiomycetous anamorphic yeast genusTrichosporon were determined, and phylogenetic trees were constructed by the neighbor-joining and maximum likelihood methods. The sequence data showed that, with the exception ofT. pullulans, the genus is monophyletic, although its members have two different major ubiquinones, Q9 and Q10. The genus can be divided phylogenetically into three major clusters. Species with Q10 as the major ubiquinone constitute a single cluster, while those with Q9 form two clusters.Trichosporon pullulans was phylogenetically distinct from other taxa of the genus. It is located in a cluster containingCystofilobasidium capitatum, Mrakía frigida, Xanthophyllomyces dendrorhous and three species ofUdeniomyces. This result sugests thatT. pullulans does not belong to the genusTrichosporon.
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  • 8
    ISSN: 1618-2545
    Keywords: Candida fukazawae ; Candida fungicola ; Candida sagamina ; new yeast species ; SSU rDNA sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four strains of anamorphic yeasts isolated from fruit bodies of mushrooms collected in Japan were found to represent three new species of the genusCandida. These species resembleCandida mesenterica in characteristics commonly employed in the classification of yeasts. On the basis of DNA-DNA reassociation, however, they were clearly distinguished fromC. mesenterica and from one another. Three new species,Candida fungicola, Candida sagamina, andCandida fukazawae, are proposed for these yeasts. The analysis of SSU rDNA sequences suggested that these three species were closely related to each other and toC. mesenterica andC. suecica.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Mycoscience 41 (2000), S. 357-369 
    ISSN: 1618-2545
    Keywords: Sporobolomyces blumeae sp. nov. ; Sporobolomyces nylandii sp. nov. ; Sporobolomyces poonsookiae sp. nov. ; Sporobolomyces vermiculatus sp. nov. ; systematics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thirteen undescribed strains of ballistoconidium-forming yeasts, isolated from leaves collected in the suburbs and along the southeast seacoast of Bangkok, Thailand, were divided into four different groups in the genusSporobolomyces on the basis of morphological, physiological, biochemical, and chemotaxonomical characteristics, and analyses of the sequences of 18S rDNA and internal transcribed spacer regions. DNA-DNA reassociation experiments with related species revealed that these four groups were four new distinct species.Sporobolomyces nylandii sp. nov.,S. poonsookiae sp. nov.,S. blumeae sp. nov. andS. vermiculatus sp. nov. are proposed for these strains.
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  • 10
    ISSN: 1618-2545
    Keywords: ballistoconidium-forming yeast ; Bullera boninensis ; Bullera schimicola ; Bullera waltii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thirteen strains of ballistoconidium-forming yeasts were isolated from leaves collected in the Ogasawara Islands, Japan. They represent three different species in the genusBullera on the basis of morphological, physiological, and biochemical characteristics, analyses of the sequences of internal transcribed spacer regions and small subunit ribosomal DNA, and a nuclear DNA-DNA hybridization study. Three new species,Bullera boninensis (five strains),B. waltii (seven strains), andB. schimicola (one strain), are proposed for these 13 strains.
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