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  • 1
    Electronic Resource
    Electronic Resource
    Immunocytochemical Study of the Ultrastructure of Peroxisomes and the Effects of Peroxisome Proliferators (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 804 (1996), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1996.tb18624.x
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  • 2
    Electronic Resource
    Electronic Resource
    Studies on mouse liver urate oxidase (1974)
    Springer
    Histochemistry and cell biology 39 (1974), S. 243-250 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine localization of urate oxidase was investigated with immunoferritin technique directly applied to ultrathin sections of fixed and frozen mouse liver tissue. The ferritin particles indicating the urate oxidase antigen were localized in microbodies, cisternae of rough- and smooth-surfaced endoplasmic reticulum (ER), and Golgi vacuoles and vesicles. In ER the particles were abundantly observed in dilated terminal portions. In addition, Golgi lamellae were slightly stained comparing with the vacuoles and vesicles. The staining with ferritin particles was inhibited by the treatment of unconjugated anti-urate oxidase before ferritin conjugate staining. From these results, the formation of microbody was discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493309
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  • 3
    Electronic Resource
    Electronic Resource
    Fine structural localization of arylsulfatase B activity in the rabbit blood platelets (1975)
    Springer
    Histochemistry and cell biology 44 (1975), S. 307-312 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fine structural localization of arylsulfatase in the rabbit blood platelets has been investigated in this study. Among many cell organellae, reaction products were exclusively observed in the alpha granules of the platelets. Whithin the alpha granules, arylsulfatase activity appeared to localize in variable patterns, i.e. reaction products confined mainly at the peripheral region in many granules, while they deposited heavily throughout the granule matrices in some others. In a blood platelets, each alpha granule showed the different staining pattern which indicated more variable functional heterogeneity in the granules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00490367
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  • 4
    Electronic Resource
    Electronic Resource
    Ultrastructural localization of catalase on ultracryotomic sections of mouse liver by ferritin-conjugated antibody technique (1974)
    Springer
    Histochemistry and cell biology 40 (1974), S. 165-174 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ferritin labeled antibody to mouse liver catalase was applied to ultracryotomic sections of mouse liver tissue. The ferritin particles indicating a catalase antigen were localized in microbodies, membran-ebound ribosomes, cisternae of endoplasmic reticulum, Golgi vacuoles and vesicles, and cytoplasmic matrix of liver cell. Infrequently, the ferritin particles were observed in a few lysosomes of the hepatic cell and the stellate cell of Kupffer and in perichromatin of the hepatic cell nucleus. To the contray, mitochondria, Golgi lamellae, plasma membranes, and most of nuclei and lysosomes were not stained by ferritin conjugate. The results were discussed with regard to enzymecytochemical and biochemical results described by several investigators.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495964
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  • 5
    Electronic Resource
    Electronic Resource
    Glomerular extracellular matrices and anionic sites in aging ddY mice: a morphometric study (1993)
    Springer
    Histochemistry and cell biology 99 (1993), S. 241-249 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A morphometric study was undertaken to examine age-related changes in glomerular ultrastructure and anionic sites in ddY male mice at various ages. A progressive increase in glomerular extracellular matrices, including thickening of the glomerular basement membrane (GBM), formation of GBM nodules, and mesangial matrix increase, was found to be the primary age-related ultrastructural change in aging mice; there were also electron-dense deposits in mesangial and subepithelial regions. The extent of GBM thickening in mice was less than was reported in rats. Rather, the GBM nodules, which had the same electron density as the lamina densa (LD) and protruded on the subepithelial side of the GBM, were more striking. Quantitative evaluation showed that GBM thickness, number and size of GBM nodules, and the area of the mesangial matrix were significantly correlated with the age of the mice. The distribution of anionic sites in the glomeruli of aging animals was described for the first time. No statistically significant differences were noted between the number of glomerular anionic sites in the different age groups. These results indicate that the increase in glomerular extracellular matrices reported in aged rats was also present in aged mice, although the extent of various changes was different. The results also indicate that this increase in glomerular extracellular matrices with age was not accompanied by significant alteration in glomerular anionic sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269142
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  • 6
    Electronic Resource
    Electronic Resource
    A new method for radioautographic observation on isolated cells (1961)
    Springer
    Histochemistry and cell biology 2 (1961), S. 255-259 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Eine neue Methode für radioautographische Beobachtung über die isolierten Zellen einiger Organe wurde dargestellt. Durch diese Methode kann man leicht zweikernigen Zellen von einkernigen in solchen Organen wie der Leber oder dem Pankreas, die häufig zweikernige Zellen haben, auf radioautographischen Präparaten unterscheiden. Dadurch kann man ohne Schwierigkeit über die Zweikernigkeit aus dem histochemischen Standpunkt studieren.
    Notes: Summary The procedure of a new method of radioautographic observation on isolated cells from the liver and the pancreas was described. Through this method binucleato cells are distinctly distinguished from mononucleate cells in radioautographs. Thus, the study on binuclearity in such organs from histochemical viewpoint has come to be carried out without difficulty.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00736502
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  • 7
    Electronic Resource
    Electronic Resource
    Demonstration of intracytoplasmic glycogen of megakaryocytes and blood platelets by means of the periodic acid-thiocarbohydrazide-silver proteinate method (1977)
    Springer
    Histochemistry and cell biology 52 (1977), S. 307-316 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The glycogen of megakaryocytes and blood platelets has been investigated in glutaraldehyde and osmium tetroxide fixed tissues by the periodic acid-thiocarbohydrazide-silver proteinate method (PA-TCH-SP). The PA-TCH-SP method involves the staining of intracytoplasmic glycogens more densely than the routine lead citrate method. Glycogen having a mean particle diameter of 21.1 nm has been shown localizing in the matrix of mature megakaryocytes, while that of glycogen in the platelets was 26.2 nm. The staining pattern of the glycogen in blood platelets was classified into three groups according to staining intensity. It is found that the PA-TCH-SP method is a very suitable one for the demonstration of intracytoplasmic glycogen from the viewpoints of reaction specificity, reproducibility, fineness of reaction products, sufficiency of electron density, and experimental cost. This method is also a very useful one for differentiating intracytoplasmic glycogens and ribosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00508403
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  • 8
    Electronic Resource
    Electronic Resource
    Histochemical studies of mucosubstances in metaplastic epithelium of the stomach, with special reference to the development of intestinal metaplasia (1978)
    Springer
    Histochemistry and cell biology 56 (1978), S. 229-237 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Processes in the development of intestinal metaplasia of the stomach were investigated from the morphological and histochemical approaches using light and electron microscopic techniques. The specimens taken from 38 gastric carcinomas and 15 gastric and/or duodenal ulcers were subjected to this study. Morphological appearances of the intestinal metaplasia observed in routine examination with hematoxylin and eosin staining was able to be divided into complete and incomplete metaplasia by the light and electron microscopic histochemical stainings of the mucosubstances. The columnar cells at the area of the incomplete metaplasia had both the properties of the intestinal epithelia and the gastric foveolar epithelia. The incomplete as well as the complete metaplasia arose from the generative cells at the isthmus of the gland. The generative cells, however, sometimes gradually transformed to produce the complete metaplastic cells. The two processes of the development of the intestinal metaplasia were proposed and discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495984
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  • 9
    Electronic Resource
    Electronic Resource
    Immunogold studies on peroxisomes: Review of the localization of specific proteins in vertebrate peroxisomes (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 31 (1995), S. 79-92 
    Details
    ISSN: 1059-910X
    Keywords: Immunogold technique ; Protein A-gold ; Peroxisome ; Subcompartment ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Peroxisomes, since their discovery as microbodies, have been studied mostly independently by electron microscopists and biochemists. The fine structure has been studied by electron microscopy, and the compositional enzymes and proteins by protein biochemistry. Electron microscopic histochemistry has been used to try to clarify the relationship between the fine structure and its constituents. The immunogold technique, a combination of electron microscopy and protein biochemistry, for the first time resolved this problem due to the high sensitivity and resolution power of the staining and the high reliability of the technique. The present paper reviews the way in which the immunogold techniques, especially the protein A-gold technique, revealed the localization of various enzymes or proteins in peroxisomes or peroxisomal subcompartments, and discusses why this technique should be employed in peroxisome research. © 1995 Wiley-Liss, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070310107
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  • 10
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    Matrix-assisted laser desorption/ionization imaging mass spectrometry revealed traces of dental problem associated with dental structure (2013)
    Springer
    Details
    Publication Date: 2013-06-02
    Print ISSN: 1618-2642
    Electronic ISSN: 1618-2650
    Topics: Chemistry and Pharmacology
    Published by Springer
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