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  • 1
    Electronic Resource
    Electronic Resource
    Synthesis by DNA polymerase I on bleomycin-treated deoxyribonucleic acid: a requirement for exonuclease III (1981)
    s.l. : American Chemical Society
    Biochemistry 20 (1981), S. 238-244 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00505a002
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  • 2
    Electronic Resource
    Electronic Resource
    Construction and characterization of centric circular and acentric linear chromosomes in fission yeast (1990)
    Springer
    Current genetics 18 (1990), S. 323-330 
    Details
    ISSN: 1432-0983
    Keywords: Artificial chromosome ; Centromere ; Genome manipulation ; Schizosaccharomyces pombe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Gene disruption and gap repair of chromosomal DNA have been frequently employed techniques in yeast genetics. To extend the possibility of using these gene manipulations for larger genomic regions, we have examined the maximal sizes of chromosomal DNA disrupted or repaired in vivo. Here we report a simple, potentially general, method for selectively deleting a 150 kb region, or gap-filling a 100 kb region, in the fission yeast genome. This enables the generation of acentric linear chromosomes by deletion, or the cloning of large functional centromeric DNAs into circular minichromosomes by gap-filling. The fidelity of the resulting gap-filling is high, judging from partial-digestion mapping of gap-repaired DNAs. By analysing a series of such circular minichromosomes, we conclude that only a part of the repetitive centromeric region, including the central domain, is essential for mitotic and meiotic chromosome segregation. Acentric linear chromosomes, although unstable, could be maintained, indicating that it may be possible to construct an acentric vector for large DNA fragments in this organism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318213
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  • 3
    Electronic Resource
    Electronic Resource
    Universal and essential role of MPF cdc2 + (1988)
    [s.l.] : Nature Publishing Group
    Nature 336 (1988), S. 430-430 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIRá€"As reported recently in News and Views1, the product of the mitosis-inducing cdc2+ gene of the fission yeast, Schizosaccharomyces pombe is a com-ponent of the maturation promoting factor (MPF)2^4, first recognized in Xenopus oocytes. This exciting finding demon-strates the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/336430a0
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  • 4
    Electronic Resource
    Electronic Resource
    Structure of the fission yeast centromere cen3: Direct analysis of the reiterated inverted region (1991)
    Springer
    Chromosoma 101 (1991), S. 214-221 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We determined the structure of the Schizosaccharomyces pombe centromere cen3 using direct genomic mapping and cosmid walking. The repetitive region of cen3 is approximately 110 kb, much longer than that of the previously determined cen1 and cen2 regions. The ≈30 kb long left and ≈60 kb right repetitive sequences are arranged with an inverted symmetry and flank the 15≈20 kb central domain. The repeat motifs in cen3, although they consist of the common centromeric repeat elements, are slightly different from those in cen1 and cen2. The cen3 repeat motifs appear to be reiterated four times in the left and nine times in the right side repetitive regions. We found that the central domain consists of the common ≈5 kb core sequence associated with the pair of innermost inverted sequences, most of which are reiterated only twice in the genome. Although their sizes differ significantly, the general features of cen1, cen2 and cen3 are similar, and a prototype, consensus structure for the fission yeast centromere may be deduced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365153
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  • 5
    Electronic Resource
    Electronic Resource
    Mapping of rRNA genes by integration of hybrid plasmids in Schizosaccharomyces pombe (1984)
    Springer
    Current genetics 8 (1984), S. 93-97 
    Details
    ISSN: 1432-0983
    Keywords: Integration vector ; Nucleolar DNA ; rRNA gene mapping ; Schizosaccharomyces pombe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The major rRNA genes of the fission yeast Schizosaccharomyces pombe were mapped on chromosome III by plasmid integration. The integration vector YIp33 containing S. cerevisiae LEU2 gene was combined with the S. pombe rDNA. Since LEU2 complements S. pombe leu1 deficiency, it could be used as the genetic marker for integration. The 10.4 kb rDNA repeat contained ARS sequence, and therefore 2.4 kb and 0.7 kb subfragments not containing ARS were subcloned into YIp33 and transformed leu1 S. pombe cells to Leu+. Genetic analyses of the transformants indicated that the integrated rDNA resides in the long arm of the shortest chromosome III, tightly linked to ade5 (1.4 cM). This result is consistent with our previous finding that the DAPI-stained smallest chromosomes were associated with the nucleolus (Umesono et al. 1983).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00420224
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  • 6
    Electronic Resource
    Electronic Resource
    Triploid meiosis and aneuploidy in Schizosaccharomyces pombe: an unstable aneuploid disomic for chromosome III (1985)
    Springer
    Current genetics 9 (1985), S. 463-470 
    Details
    ISSN: 1432-0983
    Keywords: Triploid meiosis ; Schizosaccharomyces pombe ; Unstable aneuploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Triploid meiosis in crosses between haploid and diploid strains of the fission yeast Schizosaccharomyces-pombe was investigated by tetrad analyses. Viability of the segregants was low. Only about 10% of the total tetrads contained four colony-forniing spores and most of these segregated into two haploids and two diploids. The remaining tetrads were rather normal in germination but were defective in colony formation. More than 50% of the total tetrads had no colony-forming spores while about 30% contained 1–3 colony-forming spores. Among the colony-forming segregants from the defective tetrads, a class of aneuploids disomic for the shortest chromosome III was obtained. In such aneuploid cells combined with a cold-sensitive ß-tubulin mutation, an additional short chromosome corresponding to chromosome III was observed by DAPI staining when the cells were incubated at a restrictive temperature. The other classes of aneuploids appeared able to be to germinate but not to enter into vegetative growth. Detailed genetical analyses indicated that recombination frequencies near the centromere were strikingly different between triploid and normal diploid meiosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00434051
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  • 7
    Electronic Resource
    Electronic Resource
    Fission yeast sta mutations that stabilize an unstable minichromosome are novel cdc2-interacting suppressors and are involved in regulation of spindle dynamics (1995)
    Springer
    Molecular genetics and genomics 249 (1995), S. 391-399 
    Details
    ISSN: 1617-4623
    Keywords: Schizosaccharomyces pombe ; Mitotic regulation ; sta mutations ; Minichromosome ; Cell cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cytological observations have shown that the presence of unstable minichromosomes can delay progression through the early stages of mitosis in fission yeast (Schizosaccharomyces pombe), suggesting that such minichromosomes may provide a useful tool for examining the system that regulates the coordinated segregation of chromosomes. One such unstable minichromosome is a large circular minichromosome. We previously showed that the mitotic instability of this minichromosome is probably due to the frequent occurrence of catenated forms of DNA after replication. To identify genes involved in the regulation of chromosome behavior in mitosis, we isolated mutants which stabilized this minichromosome. Three loci (stal, sta2, and sta3) were identified. Two of them were found to be suppressors of temperature-sensitive mutations in cdc2, which encodes the catalytic subunit of muturation promoting factor (MPF). They show no linkage to, and are thus different from, sucl, and cdc13, previously identified as genes that interact with cdc2. The other mutation mapped to a gene previously identified as being required for the correct formation of the mitotic spindle. Data provided in this study suggest that the sta genes are involved in the regulation of spindle dynamics to ensure proper chromosome segregation during mitosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00287100
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  • 8
    Electronic Resource
    Electronic Resource
    Sequence homology in DNA molecules of temperate phages φ81, φ80 and λ (1978)
    Springer
    Molecular genetics and genomics 159 (1978), S. 259-268 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Homology maps between bacteriophages ϕ81, ϕ80 and λ were constructed on the basis of electron microscope observation of DNA heteroduplexes. In ϕ81/ϕ80 heteroduplex, the left half and the right terminal region of 13% the total molecular length were highly homologous, while the remaining region covering the early gene cluster was entirely nonhomologous. In ϕ81/λ heteroduplex, high-degree homologies were detected at the left 14% terminal region covering the head gene cluster, the central 3.8% region covering the att-int-xis region and the 1.3% Q homology region. Low-degree homologies of shorter length were scattered at the tail gene cluster, b2 region, cIII region, PQ region and SR region. Comparing our results with the homology maps of other lambdoid phages reported by Simon et al. (1971) and Fiandt et al. (1971), a phylogenic relation of ϕ81 to other lambdoid phages and the role of recombination in the course of divergence of lambdoid phages are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268262
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  • 9
    Electronic Resource
    Electronic Resource
    Construction of a mini-chromosome by deletion and its mitotic and meiotic behaviour in fission yeast (1986)
    Springer
    Molecular genetics and genomics 203 (1986), S. 397-405 
    Details
    ISSN: 1617-4623
    Keywords: Centromere ; Mini-chromosome ; Partial aneuploidy ; Schizosaccharomyces pombe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A highly stable, partial aneuploid, HM248, of the fission yeast Schizosaccharomyces pombe was obtained from the unstable aneuploid disomic for chromosome III by γ-irradiation. It contained a 500 kb mini-chromosome (designated Ch16) that was separated as a single band by pulsed field gradient electrophoresis. Genetic analysis showed that Ch16 was deleted for most of chromosome III except for the pericentric region; three centromere-linked markers encompassing the centromere region remained. This was further substantiated by integrating the cloned fragments of Ch16 DNA extracted from the agarose gel; integrations took place in the pericentric region. A 400 kb derivative (Ch16D1) was constructed which appeared to lack a part of Ch16. A single haploid cell of S. pombe could stably maintain Ch16 and Ch16D1 in addition to the three regular chromosomes. Ch16 was visualized as a minute chromosomal body in the nucleus of a β-tubulin mutant under restrictive conditions. A single copy of Ch16 was highly stable and behaved like a natural chromosome in mitosis and meiosis. The frequency of chromosomal loss was 10-4. In meiosis, it segregated independently of the regular chromosome III. Segregation of two Ch16s per cell could be monitored by specifically marked Ch16s containing the Saccharomyces cerevisiae LEU2 gene (designated Ch16LE) of the fluorouracil resistance marker (Ch16FR). Two copies of Ch16 were mitotically unstable (chromosomal loss, 10-3) and frequently failed in meiotic segregation. The frequency of meiotic recombination between the two Ch16s was greatly reduced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422063
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  • 10
    Electronic Resource
    Electronic Resource
    A large circular minichromosome of Schizosaccharomyces pombe requires a high dose of type II DNA topoisomerase for its stabilization (1995)
    Springer
    Molecular genetics and genomics 246 (1995), S. 671-679 
    Details
    ISSN: 1617-4623
    Keywords: Circular chromosome ; Type II DNA topoisomerase ; Centromere repeat ; Schizosaccharomyces pombe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have constructed circular minichromosomes, ranging in size from 36 to 110 kb, containing the centromeric repeats of Schizosaccharomyces pombe cen3. Comparison of their mitotic stability showed that the circular minichromosomes became more unstable with increasing in size, however, a linear cen3 minichromosome, which is almost the same size as the largest circular one tested, does not show such instability. High levels of expression of the top2 + (type II DNA topoisomerase; topo II) but not top1 + gene (type I DNA topoisomerase) suppressed the instability of the largest circular minichromosome, whereas partial inactivation of topo II dramatically destabilized the minichromosome. A mutant topo II, defective in nuclear localization but still retaining its in vitro relaxation activity, did not stabilize the circular minichromosome. These results indicate that endogenous type II DNA topoisomerase is insufficient for accurate segregation of the circular minichromosome. In addition, the replication of the minichromosomal DNA appears to proceed normally, because the presence of the unstable minichromosome did not cause G2 delay. A likely cause of the instability is intertwining of the minichromosome DNA possibly occuring after DNA replication. An interaction between topo II and the centromeric repeats is implied by the finding that multiple copies of the centromeric repeat, dg-dh, affect stability of the minichromosome similarly to top2 + gene dosage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290712
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