Publication Date:
2003-04-19
Description:
The elongation phase of transcription by RNA polymerase II (RNAPII) is highly regulated and tightly linked to pre-messenger RNA (pre-mRNA) processing. Recent studies have implicated an early elongation checkpoint that facilitates the link to pre-mRNA processing. Here we show that the yeast forkhead transcription factors, Fkh1p and Fkh2p, associate with the coding regions of active genes and influence, in opposing ways, transcriptional elongation and termination. These events are coordinated with serine-5 and -2 phosphorylation of the heptad repeat of the carboxy-terminal domain (CTD) of RNAPII. Our results suggest that, in addition to their documented promoter function, Fkh1p and Fkh2p coordinate early transcription elongation and pre-mRNA processing. This may reflect a general feature of gene regulation in eukaryotes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Morillon, Antonin -- O'Sullivan, Justin -- Azad, Abul -- Proudfoot, Nicholas -- Mellor, Jane -- New York, N.Y. -- Science. 2003 Apr 18;300(5618):492-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12702877" target="_blank"〉PubMed〈/a〉
Keywords:
Cell Cycle Proteins/*metabolism
;
Cyclin B/genetics/metabolism
;
Forkhead Transcription Factors
;
Gene Expression Regulation, Fungal
;
*Genes, Fungal
;
Phosphoprotein Phosphatases/metabolism
;
Phosphorylation
;
Phosphoserine/metabolism
;
Promoter Regions, Genetic
;
RNA Polymerase II/*metabolism
;
RNA Precursors/metabolism
;
RNA, Fungal/metabolism
;
RNA, Messenger/metabolism
;
Reverse Transcriptase Polymerase Chain Reaction
;
Saccharomyces cerevisiae/enzymology/genetics/growth & development/*metabolism
;
Saccharomyces cerevisiae Proteins/genetics/*metabolism
;
Transcription Factors/*metabolism
;
*Transcription, Genetic/drug effects
;
Uracil/*analogs & derivatives/pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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