ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

Hits per page

hits 1 - 4 | 4 hits

Sorting

Unknown

Cytokine-specific transcriptional regulation through an IL-5Ralpha interacting protein (2001)

N. Geijsen ; I. J. Uings ; C. Pals ; [et al.]

American Association for the Advancement of Science (AAAS)

In: Science. 293(5532): 1136-8. doi: 10.1126/science.1059157.

add to mindlist on the mindlist

Details

Publication Date: 2001-08-11

Description: Cytokine receptors consist of multiple subunits, which are often shared between different receptors, resulting in the functional redundancy sometimes observed between cytokines. The interleukin 5 (IL-5) receptor consists of an IL-5-specific alpha-subunit (IL-5Ralpha) and a signal-transducing beta-subunit (betac) shared with the IL-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF) receptors. In this study, we sought to find a role for the cytoplasmic domain of IL-5Ralpha. We show that syntenin, a protein containing PSD-95/Discs large/zO-1 (PDZ) domains, associates with the cytoplasmic tail of the IL-5Ralpha. Syntenin was found to directly associate with the transcription factor Sox4. Association of syntenin with IL-5Ralpha was required for IL-5-mediated activation of Sox4. These studies identify a mechanism of transcriptional activation by cytokine-specific receptor subunits.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Geijsen, N -- Uings, I J -- Pals, C -- Armstrong, J -- McKinnon, M -- Raaijmakers, J A -- Lammers, J W -- Koenderman, L -- Coffer, P J -- New York, N.Y. -- Science. 2001 Aug 10;293(5532):1136-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pulmonary Diseases, Heart Lung Center Utrecht, University Medical Center, G03.550, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11498591" target="_blank"〉PubMed〈/a〉

Keywords: Animals ; B-Lymphocytes/immunology/*metabolism ; COS Cells ; Carrier Proteins/chemistry/*metabolism ; Cell Line ; Genes, Reporter ; High Mobility Group Proteins/*metabolism ; Humans ; Interleukin-5/*pharmacology ; *Intracellular Signaling Peptides and Proteins ; *Membrane Proteins ; Mice ; Mitogen-Activated Protein Kinases/metabolism ; Phosphorylation ; Point Mutation ; Protein Structure, Tertiary ; Receptors, Interleukin/chemistry/genetics/*metabolism ; Receptors, Interleukin-5 ; Recombinant Fusion Proteins/metabolism ; SOXC Transcription Factors ; Sequence Deletion ; Signal Transduction ; Syntenins ; Trans-Activators/*metabolism ; *Transcriptional Activation ; Transfection ; Two-Hybrid System Techniques

Print ISSN: 0036-8075

Electronic ISSN: 1095-9203

Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics

Published by American Association for the Advancement of Science (AAAS)

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

PAPER CURRENT

S·F·X

Fulltext

Unknown

The pluripotent genome in three dimensions is shaped around pluripotency factors (2013)

E. de Wit ; B. A. Bouwman ; Y. Zhu ; [et al.]

Nature Publishing Group (NPG)

In: Nature. 501(7466): 227-31. doi: 10.1038/nature12420.

add to mindlist on the mindlist

Details

Publication Date: 2013-07-26

Description: It is becoming increasingly clear that the shape of the genome importantly influences transcription regulation. Pluripotent stem cells such as embryonic stem cells were recently shown to organize their chromosomes into topological domains that are largely invariant between cell types. Here we combine chromatin conformation capture technologies with chromatin factor binding data to demonstrate that inactive chromatin is unusually disorganized in pluripotent stem-cell nuclei. We show that gene promoters engage in contacts between topological domains in a largely tissue-independent manner, whereas enhancers have a more tissue-restricted interaction profile. Notably, genomic clusters of pluripotency factor binding sites find each other very efficiently, in a manner that is strictly pluripotent-stem-cell-specific, dependent on the presence of Oct4 and Nanog protein and inducible after artificial recruitment of Nanog to a selected chromosomal site. We conclude that pluripotent stem cells have a unique higher-order genome structure shaped by pluripotency factors. We speculate that this interactome enhances the robustness of the pluripotent state.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉de Wit, Elzo -- Bouwman, Britta A M -- Zhu, Yun -- Klous, Petra -- Splinter, Erik -- Verstegen, Marjon J A M -- Krijger, Peter H L -- Festuccia, Nicola -- Nora, Elphege P -- Welling, Maaike -- Heard, Edith -- Geijsen, Niels -- Poot, Raymond A -- Chambers, Ian -- de Laat, Wouter -- G0901533/Medical Research Council/United Kingdom -- England -- Nature. 2013 Sep 12;501(7466):227-31. doi: 10.1038/nature12420. Epub 2013 Jul 24.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Hubrecht Institute-KNAW & University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23883933" target="_blank"〉PubMed〈/a〉

Keywords: Animals ; Binding Sites ; Cell Line ; Chromatin/*chemistry/genetics/*metabolism ; Chromatin Immunoprecipitation ; *Chromosome Positioning ; Embryonic Stem Cells/cytology/metabolism ; Enhancer Elements, Genetic ; Genome/*genetics ; Homeodomain Proteins/metabolism ; *Imaging, Three-Dimensional ; Induced Pluripotent Stem Cells/cytology/metabolism ; Mice ; Molecular Imaging ; Octamer Transcription Factor-3/metabolism ; Organ Specificity ; Pluripotent Stem Cells/*cytology/*metabolism ; Promoter Regions, Genetic ; SOXB1 Transcription Factors/metabolism

Print ISSN: 0028-0836

Electronic ISSN: 1476-4687

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics