Publication Date:
2004-05-25
Description:
The herbicide glyphosate is effectively detoxified by N-acetylation. We screened a collection of microbial isolates and discovered enzymes exhibiting glyphosate N-acetyltransferase (GAT) activity. Kinetic properties of the discovered enzymes were insufficient to confer glyphosate tolerance to transgenic organisms. Eleven iterations of DNA shuffling improved enzyme efficiency by nearly four orders of magnitude from 0.87 mM-1 min-1 to 8320 mM-1 min-1. From the fifth iteration and beyond, GAT enzymes conferred increasing glyphosate tolerance to Escherichia coli, Arabidopsis, tobacco, and maize. Glyphosate acetylation provides an alternative strategy for supporting glyphosate use on crops.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Castle, Linda A -- Siehl, Daniel L -- Gorton, Rebecca -- Patten, Phillip A -- Chen, Yong Hong -- Bertain, Sean -- Cho, Hyeon-Je -- Duck, Nicholas -- Wong, James -- Liu, Donglong -- Lassner, Michael W -- New York, N.Y. -- Science. 2004 May 21;304(5674):1151-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Verdia, Inc. Redwood City, CA 94063, USA. linda.castle@verdiainc.com〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15155947" target="_blank"〉PubMed〈/a〉
Keywords:
Acetylation
;
Acetyltransferases/chemistry/*genetics/metabolism
;
Amino Acid Sequence
;
Bacillus/enzymology
;
Catalysis
;
*DNA Shuffling
;
*Directed Molecular Evolution
;
Drug Resistance
;
Escherichia coli/genetics
;
Gene Library
;
Genetic Variation
;
Glycine/*analogs & derivatives/metabolism/*toxicity
;
Herbicides/metabolism/*toxicity
;
Kinetics
;
Molecular Sequence Data
;
Mutagenesis
;
*Plants, Genetically Modified/drug effects/genetics
;
Recombinant Proteins/metabolism
;
Recombination, Genetic
;
Tobacco/drug effects/genetics/growth & development
;
Transformation, Genetic
;
Zea mays/drug effects/genetics/growth & development
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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