ISSN:
1573-5028
Keywords:
hygromycin
;
polyadenylation site
;
tetracycline-inducible gene expression
;
tomato
;
transcriptional interference
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Tomato plants stably expressing the tetR gene were transformed with the β-glucuronidase (GUS) gene and an le20 antisense construct both under the control of the Triple-Op promoter. Induction of GUS mRNA and le20 antisense RNA in leaves was demonstrated in response to tetracycline. le20 antisense induction was also demonstrated in fruit. Activation of the Triple-Op promoter reduced expression of the downstream hygromycin phosphotransferase II (hptII) gene. Transcriptional interference was therefore observed between tandem genes within T-DNA. Such interference may occur in other T-DNAs, but would not be readily observed without an upstream promoter with controllable activity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005888616748
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