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An RNA polymerase II mutation in Drosophila melanogaster that mimics ultrabithorax (1981)

Mortin, M. A. ; Lefevre, G.

Springer

Chromosoma 82 (1981), S. 237-247

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ISSN: 1432-0886

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract An EMS-induced, sex-linked recessive lethal mutation that in heterozygous condition mimics the third-chromosome dominant mutant Ultrabithorax-130 (Ubx 130) has been discovered in Drosophila melanogaster. This Ultrabithorax-like (Ubl) mutant, when heterozygous, adds several hairs to and enlarges the apical segment (capitellum) of the haltere. Ubl fails to complement Ubl n [previously called l(1)L5], a recessive lethal null allele located in section 10C of Bridges' (1938) map of the polytene X chromosome at map position 35.7. Ubl behaves as an antimorph: heterozygous deficiencies for section 10C do not display the Ubl dominant phenotype. Ubl shows a dosage effect: the maximum expression occurs in females with the genotype Ubl/Ubl; Dp Ubl +, in which the capitellum is about three times as large as that of Ubl/ +, with two or more rows of bristles. These flies are poorly viable and sterile when mated to Ubl; Dp Ubl + males, but produce a few offspring when mated to Ubl + males. Ubl displays a complex series of interactions with loci other than Ubx and elicits expression of specific mutant phenotypes when it is heterozygous in trans with certain nonallelic deficiencies and recessive mutations. Greenleaf et al. (1980) have demonstrated that Ubl is allelic with an α-amanitin-resistant mutation that affects RNA polymerase II; therefore, the interactions observed between Ubl and other loci may result from an inability of heterozygous Ubl flies to undergo normal transcription.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00286108

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Selection and analysis of rare second-site suppressors of Drosophila RNA polymerase II mutations (1998)

Krasnoselskaya, I. ; Huang, J. ; Jones, T. ; [et al.]

Springer

Molecular genetics and genomics 258 (1998), S. 457-465

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ISSN: 1617-4623

Keywords: Key words Adaptive mutation ; Drosophila melanogaster ; Mutagenesis ; RNA polymerase II ; Suppressor selection

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract We used a mutagenesis and selection procedure in Drosophila melanogaster to recover rare allele-specific suppressor mutations. More than 11 million flies mutant for one of five recessive-lethal mutations in the two largest subunits of RNA polymerase II were selected for additional mutations that restored viability. Forty-one suppressor mutations were recovered. At least 16 are extragenic, identifying a minimum of three loci, two of which do not map near genes known to encode subunits of RNA polymerase II. At most, 25 are intragenic, 4 reverting the initial altered nucleotide back to wild type. Sequence analysis of interacting mutations in the two largest subunits identified a discrete domain in each subunit. These domains might be contact points for the subunits. Finally, our selections were large enough to allow recovery of multiple independent changes in the same nucleotides yet mutations in other equally likely targets were not recovered. The mutations recovered are not random and might provide insights into possible mechanisms for mutagenesis in eukaryotes.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s004380050756

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Genomic organization of the segment polarity gene pan in Drosophila melanogaster (1998)

Dooijes, D. ; van Beest, M. ; van de Wetering, M. ; [et al.]

Springer

Molecular genetics and genomics 258 (1998), S. 45-52

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ISSN: 1617-4623

Keywords: Key wordsDrosophila melanogaster ; High mobility group proteins (HMG) ; Lymphoid enhancer binding protein (LEF) ; T cell factor (TCF) ; Wnt/wingless signaling

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract We previously described the molecular cloning of a mammalian T cell factor 1 (TCF-1)-like protein from Drosophila melanogaster, encoded by the pangolin (pan) locus, and demonstrated that it consists of a DNA binding domain similar to that of other high mobility group proteins and a protein-protein interaction domain that binds β-catenin (Armadillo in Drosophila) but that it lacks a transcriptional activation domain. Here we show that the pan locus spans approximately 50 kb and the mRNA results from the splicing of 13 exons. We note remarkable conservation of the exon/intron boundaries between the human and D. melanogaster genes, suggesting that they share a common ancestor. Chromosomal in situ hybridization locates pan to the base of chromosome 4, near the cubitus interruptus locus. Restriction map and sequence analyses confirm their close proximity. The small fourth chromosome undergoes little or no recombination and was previously reported to lack DNA polymorphisms; however, we note two DNA polymorphisms occurring in three combinations within the pan locus, demonstrating the presence of synonymous substitutions and the past occurrence of recombination. We present evidence suggesting that the protein encoded by pan is more similar to mammalian TCF-1 and Caenorhabditis elegans POP-1 than to mammalian LEF-1.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s004380050705

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