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  • 1
    Electronic Resource
    Electronic Resource
    Endogenous abscisic acid and wheat germ agglutinin content in wheat grains during development (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 77 (1989), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abscisic acid (ABA) and wheat germ agglutinin content of immature wheat grains and embryos was determined by immunoassay throughout the development of a field-grown wheat crop (Triticum aestivum cv. Timmo). Wheat germ agglutinin accumulation in the embryo was not preceded by an increase in endogenous abscisic acid amount or concentration in either embryos or grains. At a later stage in development the endogenous concentration of abscisic acid in both embryos and grains was found to be two orders of magnitude lower than the endogenous levels required to inhibit precocious germination and promote wheat germ agglutinin accumulation in excised embryos cultured in vitro. These findings are discussed in the context of the control of embryo development in vivo by both ABA and the water status of the grain and embryo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1989.tb05384.x
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  • 2
    Electronic Resource
    Electronic Resource
    Current advances in abscisic acid action and signalling (1994)
    Springer
    Plant molecular biology 26 (1994), S. 1557-1577 
    Details
    ISSN: 1573-5028
    Keywords: abscisic acid ; gene regulation ; mutants ; seeds ; stomata ; stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Abscisic acid (ABA) participates in the control of diverse physiological processes. The characterization of deficient mutants has clarified the ABA biosynthetic pathway in higher plants. Deficient mutants also lead to a revaluation of the extent of ABA action during seed development and in the response of vegetative tissues to environmental stress. Although ABA receptor(s) have not yet been identified, considerable progress has been recently made in the characterization of more downstream elements of the ABA regulatory network. ABA controls stomatal aperture by rapidly regulating identified ion transporters in guard cells, and the details of the underlying signalling pathways start to emerge. ABA actions in other cell types involve modifications of gene expression. The promoter analysis of ABA-responsive genes has revealed a diversity of cis-acting elements and a few associated trans-acting factors have been isolated. Finally, characterization of mutants defective in ABA responsiveness, and molecular cloning of the corresponding loci, has proven to be a powerful approach to dissect the molecular nature of ABA signalling cascades.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016490
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  • 3
    Electronic Resource
    Electronic Resource
    Gene targeting in Arabidopsis thaliana (1992)
    Springer
    Molecular genetics and genomics 231 (1992), S. 186-193 
    Details
    ISSN: 1617-4623
    Keywords: Arabidopsis thaliana ; Homologous recombination ; Protoplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Gene targeting of a chromosomally integrated transgene in Arabidopsis thaliana is reported. A chimeric gene consisting of the promoter of the 35S RNA of CaMV, the polyadenylation signal of the octopine synthase gene and the coding region of the bacterial hygromycin phosphotransferase gene (hpt), which was rendered non-functional by deletion of 19 bp, was introduced into the genome of A. thaliana using Agrobacterium-mediated gene transfer. A total of 3.46 x 108 protoplasts isolated from 17 independent transgenic Arabidopsis lines harbouring the defective chimeric hpt gene were transformed via direct gene transfer using various DNA forms containing only the intact coding region of the hpt gene. Out of 150 hygromycin-resistant colonies appearing in the course of these experiments, four were the result of targeted recombination of the incoming DNA with the defective chromosomal locus as revealed by PCR and Southern blot analysis. Comparison with the number of transformants obtained when an hpt gene controlled by a promoter and terminator from the nopaline synthase gene was employed results in a maximal ratio of homologous to non-homologous transformation in A. thaliana of 1 x 10−4.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279790
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  • 4
    Electronic Resource
    Electronic Resource
    Transient expression in leaf mesophyll protoplasts of Arabidopsis thaliana (1994)
    Springer
    Plant cell, tissue and organ culture 36 (1994), S. 53-58 
    Details
    ISSN: 1573-5044
    Keywords: Arabidopsis thaliana ; GUS ; protoplast ; transient expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Conditions for maximising transient expression of GUS in leaf mesophyll protoplasts of Arabidopsis thaliana ecotype C24 were investigated. It was found that the factors most influencing expression levels, with optimum levels in parenthesis, were plasmid DNA quantity (100 μg per 5 × 105 protoplasts), inclusion of carrier DNA (50 μg), PEG pH and amount (pH above 6, and total PEG concentration at least 9% w/w) and the topological form of the DNA. Linearised plasmid DNA with long flanking sequences 3′ and 5′ to the marker gene yielded the highest levels of GUS expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048315
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  • 5
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    Arabidopsis ABA Response Gene ABI1 : Features of a Calcium-Modulated Protein Phosphatase (1994)
    American Association for the Advancement of Science
    Details
    Publication Date: 1994-06-03
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science
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