ISSN:
1432-203X
Keywords:
Triticum aestivum
;
microspore culture
;
ovary co-culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Wheat microspores were isolated, without prior anther culture, from a range of genotypes and cultured to regenerate self-fertile plants. Microspores were isolated using a microblender and competent microspores were enriched by gradient centrifugation. The use of maltose as the sole carbohydrate in the culture medium and co-culture of microspores with wheat or barley ovaries were critical for development of microspore-derived embryos. Results were also improved when spikes were pretreated by emersion of the basal ends of detached heads in water at 25°C for 2d. This procedure leads to highly reproducible production of plants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00239096
Permalink