ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Despite many outstanding research works on cartilage tissue engineering, actualclinical application is not quite successful because of the absorption and progressive distortion oftissue engineered cartilage. We have developed a new method of cartilage tissue engineeringcomprising chondrocyte mixed Pluronic F-127 and cultured chondrocyte cell sheet which entirelycover the cell-Pluronic complex. We believe the addition of cultured chondrocyte cell sheetenhances the efficacy of chondrogenesis in vivo.Human ear cartilage piece was enzymatically dissociated and chondrocyte suspension wasacquired. Chondrocytes were cultured and expanded as the routine manner. Cultured chondrocyteswere plated in high-density monolayer and cultured with Chondrogenic media in 5% CO2 incubator.After 3 weeks of culture, chondrocyte cell sheet was formed and complete single sheet ofchondrocyte could be harvested by gentle manipulation of culture plate with a cell scraper.Chondrocyte-Pluronic complex was established by mixing 1x 106 cells with 0.5[removed info] of Pluronic F-127. Chondrocyte-Pluronic complex was completely covered with a sheet of cultured chondrocyte.The completed tissue engineered constructs were implanted into the subcutaneous tissue pocket ofnude mice on the back. Tissue engineered constructs without cultured cell sheet were used ascontrol. Samples were harvested at 8 weeks postoperatively and they were subjected to histologicalanalysis and assayed for glycosaminoglycan (GAG), and type II collagen. Grossly, the size ofcartilage specimen of cultured chondrocyte cell sheet covered group was larger than that of thecontrol. On histologic examination, the specimen of cultured chondrocyte cell sheet covered groupshowed lacunae-containing cells embedded in a basophilic matrix. The chondrocyte cell sheetcovered group specimen resembled mature or immature cartilage. The result of measurement ofGAG and type II collagen of cartilage specimen of cultured chondrocyte sheet covered group washigher than that of the control.In conclusion, the new method of cartilage tissue engineering using chondrocyte cell sheet seemsto be an effective method providing higher cartilage tissue gain and reliable success rate forcartilage tissue engineering
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.89.pdf
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