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  • 1
    Electronic Resource
    Electronic Resource
    Breeding value of 2n pollen (diplandroids) in tetraploid x diploid crosses in potatoes (1975)
    Springer
    Theoretical and applied genetics 46 (1975), S. 307-314 
    Details
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The relative breeding value of first division restitution (FDR) and second division restitution (SDR) 2n male gametes from diploids, and n (2x) gametes from tetraploids were compared. This was done by measuring tuber yield of 105 4x families obtained from 4x x 2x (FDR), 4x x 2x (SDR), and 4x x 4x crosses at two locations. All tetraploid families obtained from 4x x 2x (FDR) matings exhibitedheterosis (the mean of the F1 exceeded that of the higher yielding parent), and outyielded other tetraploid families and 4x cultivars at both locations. The tuber yield of 4x x 2x (SDR; families was higher than that of the families derived from 4x x 4x matings, although the 2x parents had significantly lower yield than tetraploid parents. Specific combining ability (S.C.A.) was found to be significant when families obtained from each category (4x x FDR, 4x x SDR and 4x x 4x) were analyzed individually. Both general combining ability (G.C.A.) (2x) and S.C.A. were significant when families obtained from: 1) 4x x FDR plus 4x x SDR; 2) 4x x FDR plus 4x x 4x; and 3) all categories, were analyzed. Only S.C.A. was found to be significant when 4x x SDR and 4x x 4x families were analyzed together. Consistent results were obtained at each of, and over, the two locations. These results strongly demonstrated that FDR gametes were superior in breeding value to either SDR or n (2x) gametes, and were more homogeneous, Gametes formed by SDR might be superior to n (2x) gametes although they were as heterogeneous. Distinct yield differences between 4x x FDR and 4x x SDR families plus the significant G.C.A. (2x) detected when these two categories were compared, substantiated the cytological interpretation of genetically distinct modes of diplandroid formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00281153
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  • 2
    Electronic Resource
    Electronic Resource
    Fertilization and early embryo development in reciprocal interspecific crosses of Phaseolus (1979)
    Springer
    Theoretical and applied genetics 54 (1979), S. 55-59 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus ; Interspecific hybridization ; Embryo development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fertilization and early embryo and endosperm development were examined in Phaseolus vulgaris x P. acutifolius, P. vulgaris x P. lunatus crosses and their reciprocals. The number and length of pollen tubes were not different between selfings and interspecific crosses. Fertilization was completed in all matings and the time of fertilization was maternally dependent which may reflect the degree of maturation of embryo sacs at pollination. A large difference between reciprocal crosses was found in the time of endosperm and embryo division in relation to the time of fertilization. When P. vulgaris was the female parent and P. acutifolius the male parent, endosperm division occurred at the same time as in P. vulgaris upon selfing, while in P. vulgaris x P. lunatus crosses the time of endosperm division was intermediate as compared with the two parents. The time lapse between fertilization and endosperm and embryo division in P. acutifolius x P. vulgaris crosses was longer than in either parent upon selfing. In P. lunatus x P. vulgaris crosses, endosperm division occurred in only 7–12% of the ovules at 72 hours after pollination. Embryo development in these ovules was limited to the four cell stage although the endosperm was at the free nuclei stage. The severe delay in embryo and endosperm divisions may be the major cause of early pod abscission in P. lunatus x P. vulgaris crosses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265469
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  • 3
    Electronic Resource
    Electronic Resource
    Interspecific hybridization between Vigna radiata (L.) Wilczek and V. glabrescens (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 641-647 
    Details
    ISSN: 1432-2242
    Keywords: Interspecific gene transfer ; Embryo culture ; Vigna radiata ; Vigna glabrescens ; Mungbean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Interspecific hybrids of the mungbean, Vigna radiata (L.) Wilczek (2n=22) and V. glabrescens (2n=44) were generated with the aid of embryo culture. V. glabrescens x V. radiata hybrids were recovered via germination of the immature embryos. Reciprocal hybrids were obtained via shoot formation from embryonic callus. The authenticity of the hybrids was determined by morphological characteristics, chromosome number, and isozyme patterns. The hybrids were highly sterile upon selfing, but backcrossing to the diploid parent yielded viable seeds. Some of the plants resembled the diploid parent morphologically while others resembled neither parent. The backcross plants were sufficiently fertile to give a large number of mature, selfed seeds. Plants obtained differed morphologically and in their isozyme patterns from either parent, indicating introgression. These progeny populations will be used as bridging materials to transfer pest resistance from the wild tetraploid to the cultivated mungbean.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262558
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  • 4
    Electronic Resource
    Electronic Resource
    Monosomics in common bean, Phaseolus vulgaris (1977)
    Springer
    Theoretical and applied genetics 49 (1977), S. 145-149 
    Details
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two monosomics of Phaseolus vulgaris (2n = 22) were found among selfed progeny of plants treated with colchicine. The monosomic chromosomes involved were identified as chromosomes H and J according to the previously suggested Giemsa karyotype. Both monosomic plants had slower growth rate and smaller size as compared with their respective euploid sibs. However, no apparent morphological characteristics distinguished the two monosomics. The frequencies of transmission through selfing of monosomics H and J were 9% and 10 % respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00281712
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  • 5
    Electronic Resource
    Electronic Resource
    Interspecific hybridization of phaseolus vulgaris with P. lunatus and P. acutifolius (1978)
    Springer
    Theoretical and applied genetics 52 (1978), S. 209-215 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus ; Interspecific hybridization ; Embryo culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The influence of genotypic combinations on the growth of hybrid embryos between Phaseolus vulgaris and P. lunatus, and between P. vulgaris and P. acutifolius was examined. All embryos obtained from P. vulgaris × P. lunatus crosses developed only to a stage which appears to be comparable to the pre-heart-shape stage of selfed embryos. Reciprocal crosses were attempted, but pods abscised at a very early stage. Embryos derived from P. vulgaris × P. acutifolius and reciprocal crosses attained the cotyledon stage although no mature seeds were formed. A distinct characteristic of these embryos was the uneven development of the two cotyledons. The rate of growth and final size of these hybrid embryos seemed to be influenced by the genotypes of both parents. Immature embryos were cultured on defined medium and the effects of glutamine and gibberellin (GA3) were examined. Glutamine was effective in increasing the survival rate; gibberellin had no apparent effect. Plants derived from cultured embryos of P. vulgaris × P. lunatus, P. vulgaris × P. acutifolius and P. acutifolius × P. vulgaris were obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273891
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  • 6
    Electronic Resource
    Electronic Resource
    Meiosis and fertility of interspecific hybrids between Phaseolus vulgaris L. and P. acutifolius A. Gray (1980)
    Springer
    Theoretical and applied genetics 57 (1980), S. 59-64 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus ; Interspecific hybridization ; Meiosis ; Fertility ; Beans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Meiosis and fertility of interspecific hybrids obtained from reciprocal crosses between Phaseolus vulgaris and P. acutifolius were examined. Bivalents as well as univalents were found at Metaphase I. The majority of the microsporocytes had four or more univalents and the average was 6.3 univalents per cell. The average number of lagging chromosomes at Anaphase I was 2.3 per cell and the most frequent chromosome distribution at late Anaphase I was 10–12. The lower than expected number of lagging chromosomes as compared with the number of univalents at Metaphase I suggests the possible occurrence of precocious separation of bivalents. The male fertility as measured by pollen stainability was 17%, however, the frequency of pollen germination in selfing was 3.5%. Upon selfing of the interspecific hybrids, no dividing embryos were found even though 7 and 26% of the ovules were fertilized at 12 hours and four days after pollination. In backcrosses to P. vulgaris (male), 6 and 20% of the ovules were fertilized and 0 and 4% of the ovules contained dividing embryos at the same sampling times. When P. acutifolius was the male parent, respective values were 8 and 31% for fertilization and 0 and 13% for ovules with dividing embryos. The frequencies of backcross embryos recovered at 14–26 days were in agreement with the frequencies of dividing embryos at four days. The ability to obtain backcross plantlets demonstrates the feasibility to further utilize interspecific hybrids for the improvement of P. vulgaris
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276402
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  • 7
    Electronic Resource
    Electronic Resource
    Meiosis and fertility of interspecific hybrids betweenPhaseolus vulgaris L. andP. acutifolius A. Gray (1980)
    Springer
    Theoretical and applied genetics 57 (1980), S. 59-64 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus ; Interspecific hybridization ; Meiosis ; Fertility ; Beans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Meiosis and fertility of interspecific hybrids obtained from reciprocal crosses betweenPhaseolus vulgaris andP. acutifolius were examined. Bivalents as well as univalents were found at Metaphase I. The majority of the microsporocytes had four or more univalents and the average was 6.3 univalents per cell. The average number of lagging chromosomes at Anaphase I was 2.3 per cell and the most frequent chromosome distribution at late Anaphase I was 10–12. The lower than expected number of lagging chromosomes as compared with the number of univalents at Metaphase I suggests the possible occurrence of precocious separation of bivalents. The male fertility as measured by pollen stainability was 17%, however, the frequency of pollen germination in selfing was 3.5%. Upon selfing of the interspecific hybrids, no dividing embryos were found even though 7 and 26% of the ovules were fertilized at 12 hours and four days after pollination. In backcrosses toP. vulgaris (male), 6 and 20% of the ovules were fertilized and 0 and 4% of the ovules contained dividing embryos at the same sampling times. WhenP. acutifolius was the male parent, respective values were 8 and 31% for fertilization and 0 and 13% for ovules with dividing embryos. The frequencies of backcross embryos recovered at 14–26 days were in agreement with the frequencies of dividing embryos at four days. The ability to obtain backcross plantlets demonstrates the feasibility to further utilize interspecific hybrids for the improvement ofP. vulgaris
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00745030
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  • 8
    Electronic Resource
    Electronic Resource
    Embryo development in reciprocal crosses of Phaseolus vulgaris L. and P. coccineus Lam. (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 59-64 
    Details
    ISSN: 1432-2242
    Keywords: Interspecific hybridization ; Embryogeny ; Male fertility ; Phaseolus ; Endospermembryointeraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Embryo development was examined in reciprocal crosses of Phaseolus vulgaris cv. ‘Great Northern’ and P. coccineus cv. ‘Scarlet Runner’. The formation of abnormal (shrunken and underdeveloped) embryos constituted the primary crossing barrier between the two species when P. coccineus was the female parent. Plants of P. coccineus X P. vulgaris were obtained by embryo culture. Although the P. vulgaris X P. coccineus cross resulted in normal seed development, the fertility of the resulting hybrids was much lower (27%) than that of the reciprocal hybrids (81%). Three classes of F2 embryos, normal, shrunken, and underdeveloped were formed on reciprocal F1s and the frequencies did not differ between reciprocal populations. Thus, the interactions between embryo and endosperm and/or maternal parent rather than cytoplasmic-nuclear effects seem to be important in the determination of the extent of embryo growth. The examination of pollen fertility of F2 plants and the development of F2 and F3 embryos suggests that the formation of abnormal embryos and reduced male fertility are independent events. The P. vulgaris — P. coccineus crosses may be useful in studying the possible involvement of interspecific differences in hormonal metabolism in the development of hybrid embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276284
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  • 9
    Electronic Resource
    Electronic Resource
    Analyses of Phaseolus vulgaris L. and P. coccineus Lam. hybrids by RFLP: preferential transmission of P. vulgaris alleles (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 703-709 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus interspecific hybrid ; RFLP ; Beans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment length polymorphism (RFLP) was determined among P. vulgaris genotypes and Phaseolus species using 19 probes. The incidence of polymorphism was high (70–86%) between species, but relatively low (22–26%) between genotypes of P. vulgaris. Suitable probes were identified for the analysis of P. vulgaris and P. coccineus hybrids. The segregation pattern in F2 populations was Mendelian for two probes (LHB and VEE20) and non-Mendelian for GS-g, CHS, and CHI. Statistical analyses indicated gametic selection with preferential transmission of the P. vulgaris alleles, which may account for the selective recovery of P. vulgaris progeny types observed earlier. The available hybrids of P. vulgaris and P. coccineus and the high degree of interspecific RFLP will facilitate the construction of a linkage map for Phaseolus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226740
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  • 10
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis and shoot organogenesis from interspecific hybrid embryos of Vigna glabrescens and V. radiata (1990)
    Springer
    Plant cell reports 9 (1990), S. 77-79 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Somatic embryogenesis occurred on cotyledons of morphologically abnormal embryos derived from Vigna glabrescens x V. radiata crosses and cultured on Murashige and Skoog (MS) medium without growth regulators. The frequency of 15–17 day old embryos that gave rise to somatic embryos increased from 8% to 29% by application a mixture of 100 mg/l gibberelllc acid, 25 mg/l α-naphthaleneacetic acid (NAA) and 5 mg/l kinetin daily to the pedicels of the developing pods. However, only callus formed on immature hybrid embryos of the reciprocal cross. These callus tissues occasionally gave rise to shoots via organogenesis when transferred to MS medium with 2 mg/l N6-benzyladenine and 0.05 mg/l NAA. Treatment of pods with growth regulators did not influence the frequency of organogenic callus. Selfed embryos of the parents did not form somatic embryos in culture, nor did callus derived from the selfed embryos produce shoots. Thus, the ability to redifferentiate appears to be associated with interspecific hybridity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231553
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