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  • 1
    Electronic Resource
    Electronic Resource
    A hydrogen-bonding network modulating enzyme function: asparagine-194 and tyrosine-225 of Escherichia coli aspartate aminotransferase (1993)
    s.l. : American Chemical Society
    Biochemistry 32 (1993), S. 1810-1815 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00058a015
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  • 2
    Electronic Resource
    Electronic Resource
    Heterotypic induction of stomach-like glands in the urogenital sinus endoderm under the influence of stomach mesenchyme of foetal rats (1990)
    Springer
    Development genes and evolution 198 (1990), S. 483-487 
    Details
    ISSN: 1432-041X
    Keywords: Mesenchymal-epithelial interaction ; Stomach ; Heterotypic induction ; Pepsinogen ; Morphogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Urogenital sinus endoderm of 16.5-day rat foetuses was combined with stomach mesenchyme and the recombinants were either treated with testosterone and grown in vitro or cultured beneath the kidney capsule of adult male rats of the same strain. It was found that testosterone stimulated mitosis in the urogenital endoderm. In recombinants grown under the kidney capsule a stratified squamous epithelium and stomach-like glands were induced under the influence of the forestomach and glandular stomach mesenchymes. However, the induced glands expressed neither rat pepsinogen nor rat ventral prostatic antigen. They did not produce mRNA for the prostatic steroid-binding protein C1. Thus, stomach mesenchyme of rat foetuses induces organ-specific morphogenesis but not functional differentiation in the heterologous endoderm, indicating that cytodifferentiation does not always accompany morphogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00399060
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  • 3
    Electronic Resource
    Electronic Resource
    Demonstration of sucrase immunoreactivity of the brush border induced by duodenal mesenchyme in chick stomach endoderm (1992)
    Springer
    Development genes and evolution 201 (1992), S. 389-392 
    Details
    ISSN: 1432-041X
    Keywords: Sucrase-Brush border ; Mesenchymal induction ; Stomach endoderm ; Immunoelectron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary When stomach endoderm of chick embryos was recombined and cultured with duodenal mesenchyme, the endoderm developed a brush border structure over a large area and also differentiated into mucous cells in a small area according to its own developmental fate. In the present investigation, we examined whether the induced brush border structure expressed sucrase antigen by immunoelectron microscopy using the antiserum raised against chicken sucrase. Sucrase immunoreactivity could be detected as ferritin particles in the region where the brush border was induced, whereas it was never detected on microvilli of endodermal cells which differentiated into the mucous cells. Thus, almost all of the endodermal cells could be identified as either small intestine-type cells possessing the sucrase antigen or stomach-type cells possessing mucous granules but not the sucrase antigen. The results indicate that stomach endodermal cells of chick embryos can differentiate not only morphologically but also functionally into typical intestinal epithelial cells under the inductive influence of the duodenal mesenchyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365127
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  • 4
    Electronic Resource
    Electronic Resource
    Gizzard epithelium of chick embryos can express embryonic pepsinogen antigen, a marker protein of proventriculus (1986)
    Springer
    Development genes and evolution 195 (1986), S. 475-483 
    Details
    ISSN: 1432-041X
    Keywords: Epithelial-mesenchymal interaction ; Pepsinogen expression ; Stomach ; Proventriculus ; Gizzard
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The avian stomach is composed of two distinct organs, the proventriculus and the gizzard. Pepsinogen expression in the proventricular and gizzard epithelia of chick embryos was investigated immunohistochemically with anti-embryonic chick pepsinogen (anti-ECPg) antiserum. In normal development, the ECPg antigen was expressed only in the glandular epithelial cells of the embryonic proventriculus from the 8th day of incubation onwards. However, both proventricular and gizzard epithelia of 6-day embryos expressed the ECPg antigen when recombined and cultured with the proventricular mesenchyme. Chronological studies revealed that the ECPg antigen was first detected in a few epithelial cells at 3 days of cultivation. The percentage of ECPg-positive cells among the total epithelial cells in each recombinant increased with the length of the culture period and all the glandular epithelial cells were positive at 9 days. During this process, the percentage of ECPg-positive cells in each cultured recombinant was similar in proventricular and gizzard epithelia. Moreover, both epithelia could express the ECPg antigen when recombined and cultured with the oesophageal or small-intestine mesenchyme for 9 days, though the percentage of ECPg-positive cells in each cultured recombinant was much lower than that in the cultured recombinant with the proventricular mesenchyme. These results indicate that the gizzard epithelium of 6-day chick embryos possesses a similar potential for pepsinogen expression as the proventricular epithelium of the same age.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00375887
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  • 5
    Electronic Resource
    Electronic Resource
    Developmental changes in the ability to express embryonic pepsinogen in the stomach epithelia of chick embryos (1988)
    Springer
    Development genes and evolution 197 (1988), S. 56-62 
    Details
    ISSN: 1432-041X
    Keywords: Epithelial-mesenchymal interaction ; Pepsinogen expression ; Differentiation potency ; Stomach ; Gizzard
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The avian stomach is subdivided into two parts, the proventriculus and the gizzard. It has been shown that the gizzard epithelium can express embryonic chick pepsinogen (ECPg) antigen, a marker protein of the proventricular epithelium, as well as normal proventricular epithelium, under the appropriate experimental conditions. To study the possible mechanisms involved in the suppression of ECPg synthesis in the gizzard epithelium during normal development, we carried out heterotypic and heterochronic recombination experiments of the epithelium and mesenchyme of these two organ rudiments. When recombined and cultured with 6-day proventricular mesenchyme, gizzard epithelium of 3.5- to 12-day embryos expressed pepsinogen at all stages tested. However, the ratio of ECPg-positive cells to total epithelial cells in the gizzard epithelium decreased rapidly when epithelium older than 7 days was cultured with proventricular mesenchyme. In contrast to proventricular mesenchyme, 6-day gizzard mesenchyme did not allow ECPg expression in associated proventricular epithelium of 3.5- to 7-day embryos. These results indicate that gizzard epithelium does not express pepsinogen in normal development because of both a decrease in ability to express the enzyme in itself in the course of development and a repressive influence of gizzard mesenchyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00376042
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  • 6
    Electronic Resource
    Electronic Resource
    Heterotypic differentiation of chick allantoic endoderm under the influence of various mesenchymes of the digestive tract (1974)
    Springer
    Development genes and evolution 174 (1974), S. 107-116 
    Details
    ISSN: 1432-041X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Dissociation and reassociation experimentsin vitro were carried out to investigate the differentiation potency of chick allantoic endoderm under the influence of digestive tract mesenchymes. 1. The allantoic endoderm, when cultured alonein vitro, shows no differentiation whatsoever. 2. The allantoic endoderm, when cultivated combined with the mesenchyme of oesophagus, can differentiate into a stratified cuboidal epithelium, similar to that of the embryonic oesophagus. 3. Cultivation of the allantoic endoderm combined with the proventricular mesenchyme causes differentiation of cylindrical epithelium and glands, which are characteristic of the embryonic proventriculus. 4. The combination of the allantoic endoderm and the gizzard mesenchyme results in the differentiation of pseudostratified columnar epithelium, the cells of which possess glycogen granules like those in the normal embryonic gizzard. 5. If the allantoic endoderm is cultured on the mesenchyme of the small intestine, the endodermal cells are converted into simple columnar epithelial cells similar to those of normal embryonic small intestine. 6. The competence for the heterotypic development of the allantoic endoderm appears to be a function of a developmental time sequence: it is highest in the youngest (3-day) allantoic endoderm, and gradually lost in older embryos. 7. In all combinations tested, there appear goblet cells in the epithelium when the explants are cultured more than 10 days. These cells are never observed in intact oesophagus, proventriculus and gizzard, whether in normal development or in culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00573624
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  • 7
    Electronic Resource
    Electronic Resource
    Chronological analysis of the intestinalization of chick stomach endoderm induced in vitro by duodenal mesenchyme (1985)
    Springer
    Development genes and evolution 194 (1985), S. 301-305 
    Details
    ISSN: 1432-041X
    Keywords: Intestinal induction ; Organ culture ; Stomach endoderm ; ultrastructure ; Cell contacts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Inductive action of duodenal mesenchyme on stomach endoderm in the chick embryo was chronologically analysed in vitro by the use of electron microscopy and immunofluorescence techniques. The behaviour of the endoderm-mesenchyme interfaces was particularly studied during the induction. In recombinates of 4-day stomach endoderm and 6-day duodenal mesenchyme, all the endodermal cells were undifferentiated at the start of cultivation. Small-intestinal sucrase antigen could first be detected on the 5th day of cultivation in one-third of the stomach endoderm, and a striated border on the 7th day. With a longer cultivation period, intestine-type cells increased in number in the stomach endoderm and the density of microvilli on the apical surface became higher. At the endoderm-mesenchyme interfaces a number of direct contacts between endodermal and mesenchymal cells were observed from the beginning to the end of cultivation. These were especially abundant in the early period before the appearance of signs of intestinal cytodifferentiation. These results suggest that the mesenchymal cells adjacent to the endodermal tissue play an important role in the intestinal induction which occurs during the early period of cultivation, probably via direct cell-to-cell contracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01152176
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  • 8
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    Seven-Signal Proteomic Signature for Detection of Operable Pancreatic Ductal Adenocarcinoma and Their Discrimination from Autoimmune Pancreatitis (2012)
    Hindawi
    Details
    Publication Date: 2012-06-19
    Description: There is urgent need for biomarkers that provide early detection of pancreatic ductal adenocarcinoma (PDAC) as well as discrimination of autoimmune pancreatitis, as current clinical approaches are not suitably accurate for precise diagnosis. We used mass spectrometry to analyze protein profiles of more than 300 plasma specimens obtained from PDAC, noncancerous pancreatic diseases including autoimmune pancreatitis patients and healthy subjects. We obtained 1063 proteomic signals from 160 plasma samples in the training cohort. A proteomic signature consisting of 7 mass spectrometry signals was used for construction of a proteomic model for detection of PDAC patients. Using the test cohort, we confirmed that this proteomic model had discrimination power equal to that observed with the training cohort. The overall sensitivity and specificity for detection of cancer patients were 82.6% and 90.9%, respectively. Notably, 62.5% of the stage I and II cases were detected by our proteomic model. We also found that 100% of autoimmune pancreatitis patients were correctly assigned as noncancerous individuals. In the present paper, we developed a proteomic model that was shown able to detect early-stage PDAC patients. In addition, our model appeared capable of discriminating patients with autoimmune pancreatitis from those with PDAC.
    Print ISSN: 2090-2166
    Electronic ISSN: 2090-2174
    Topics: Biology , Chemistry and Pharmacology
    Published by Hindawi
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  • 9
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    Seven-Signal Proteomic Signature for Detection of Operable Pancreatic Ductal Adenocarcinoma and Their Discrimination from Autoimmune Pancreatitis (2013)
    Hindawi
    Details
    Publication Date: 2013-01-22
    Description: There is urgent need for biomarkers that provide early detection of pancreatic ductal adenocarcinoma (PDAC) as well as discrimination of autoimmune pancreatitis, as current clinical approaches are not suitably accurate for precise diagnosis. We used mass spectrometry to analyze protein profiles of more than 300 plasma specimens obtained from PDAC, noncancerous pancreatic diseases including autoimmune pancreatitis patients and healthy subjects. We obtained 1063 proteomic signals from 160 plasma samples in the training cohort. A proteomic signature consisting of 7 mass spectrometry signals was used for construction of a proteomic model for detection of PDAC patients. Using the test cohort, we confirmed that this proteomic model had discrimination power equal to that observed with the training cohort. The overall sensitivity and specificity for detection of cancer patients were 82.6% and 90.9%, respectively. Notably, 62.5% of the stage I and II cases were detected by our proteomic model. We also found that 100% of autoimmune pancreatitis patients were correctly assigned as noncancerous individuals. In the present paper, we developed a proteomic model that was shown able to detect early-stage PDAC patients. In addition, our model appeared capable of discriminating patients with autoimmune pancreatitis from those with PDAC.
    Print ISSN: 2090-2166
    Electronic ISSN: 2090-2174
    Topics: Biology , Chemistry and Pharmacology
    Published by Hindawi
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  • 10
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    A Genetic Approach to Visualization of Multisynaptic Neural Pathways Using Plant Lectin Transgene (1999)
    Cell Press
    Details
    Publication Date: 1999-01-01
    Print ISSN: 0896-6273
    Electronic ISSN: 1097-4199
    Topics: Biology , Medicine
    Published by Cell Press
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