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  • 1
    Electronic Resource
    Electronic Resource
    A STUDY OF THE SUPERCOOLED FUSIONS AND SOLUTIONS OF SODIUM THIOSULPHATE. (1904)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 26 (1904), S. 1389-1413 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja02001a001
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  • 2
    Electronic Resource
    Electronic Resource
    Chain-transfer agents for living ROMP [Ring-Opening Metathesis Polymerization] reactions of norbornene (1990)
    s.l. : American Chemical Society
    Macromolecules 23 (1990), S. 3534-3536 
    Details
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ma00216a025
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  • 3
    Electronic Resource
    Electronic Resource
    The Determination of the Dust Fall in the Neighborhood of Cement Plants. (1914)
    s.l. : American Chemical Society
    Industrial & engineering chemistry 6 (1914), S. 454-459 
    Details
    ISSN: 1520-5045
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ie50066a003
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  • 4
    Electronic Resource
    Electronic Resource
    The Initiation and Maintenance of Vicia faba Tissue Cultures (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 34 (1975), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Explants obtained by removing the radicle tip and the plumule from embryos of Vicia faba have been induced to form callus in culture. Of a range of agar-solidified culture media tested, only that of Schenk and Hildebrandt (1972) was consistently successful. Improved growth, measured as increasing fresh weight was obtained by increasing the nitrogen content of the medium, either as potassium nitrate or as ammonium nitrate. A kinetin concentration of 0.01 mg/1 (5 × 10−8M) and a 2,4-dichlorophenoxyacetic acid (2,4-D) concentration of 0.5 mg/1 (2.3 × 10−6M) allowed optimum initial callus growth. A 2,4-D concentration of 2.3 × 10−8M, while insufficient to induce callus formation was able to inhibit lateral root development which occurred from embryo explants cultured without added 2,4-D. Subcultured tissue grew well on media supplemented with casein hydrolysate or a mixture of the eight most common amino acids in casein hydrolysate. Growth in subcultures was inhibited by two other amino acid mixtures used by other workers for different species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1975.tb03831.x
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  • 5
    Electronic Resource
    Electronic Resource
    Initiation, Growth and Nuclear Characteristics of Tissue Cultures of Paeonia suffruticosa (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 39 (1977), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Tissue cultures of the garden paeony, Paeonia suffruticosa have been established using explants of etiolated stems. Callus formation was induced on agar-solidified media containing ammonium ions or amino acids together with the hormones 2,4-dichlorophenoxyacetic acid and kinetin, but not on media lacking the reduced nitrogen component. Attempts to induce callus from explants from green plants were completely unsuccessful and were characterized by the production of intense brown colorations, both of the explant and the medium. Subcultured tissue without the added hormones produced roots, both on solid and liquid media.Growth was tested on a range of liquid media, SH/2, SH, SH × 2 and SH—M, containing 1250, 2500, 5000 and 2500 mg/l potassium nitrate. The SH—M medium also contained 1650 mg/l ammonium nitrate. Growth measured as increased fresh weight was best in the SH/2, SH and SH—M media and was curtailed in the SH × 2 medium. Soluble protein content was highest at the lowest nitrogen concentration.A histochemical comparison of tissue grown on the SH/2, SH—M and SH—M lacking hormones showed that the cells in all the cultures remained diploid while differences were established in total nuclear protein, measured using the ninhydrin-Schiff procedure. Nuclei from SH—M grown cells have a higher protein content than those from the SH/2 medium while cells from the SH—M medium lacking hormones show a further increase in nuclear protein. This raises the question whether this change in nuclear protein is related to the morphogenesis of roots which occurs in this medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1977.tb01887.x
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  • 6
    Electronic Resource
    Electronic Resource
    Developmental changes in tomato fruit composition in response to water deficit and salinity (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 83 (1991), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Processing tomato (Lycopersicon esculentum Mill. cv. UC82B) plants were subjected to moderate levels of water deficit and salinity (Na2SO4/CaCl2) in sand culture. Fruit water content and the relative contributions of organic and inorganic constituents to fruit solute potential (Ψ) and soluble solids content were determined throughout development. Fruit Ψ averaged –0.63, –0.86 and –0.77 MPa in the control, salinity and water deficit plants, respectively. Reduced net water import and maintenance of solute accumulation, irrespective of water import, accounted for the reductions in Ψ of stressed fruits. Mineral ions (Na+, K+, Ca2+, Mg2+, Cl− and SO2-4) contributed –0.31 MPa to Ψ in salinized fruit, compared with –0.19 MPa in control and water deficit treatments. Changes in net carbon accumulation were not observed among treatments, despite considerable differences in fruit K+ status. Starch accumulation in immature fruit was increased and hexose accumulation was decreased by both salinity and water deficit. Maximum starch levels were negatively correlated with total fruit Ψ, but were independent of fruit K+. Organic acid levels were generally higher throughout development in salinized plants, relative to control plants, and correlated with increased inorganic cation rather than anion accumulation in these fruits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1991.tb01299.x
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  • 7
    Electronic Resource
    Electronic Resource
    Combined staining procedures for cytophotometry of protein and DNA Feulgen-Naphthol Yellow S and dinitrofluorobenzene-Feulgen (1981)
    Springer
    Histochemistry and cell biology 73 (1981), S. 211-223 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A comparison has been made between dinitrofluorobenzene (DNFB) and Naphthol Yellow S (NYS) as protein stains in combination with the pararosaniline-SO2 Feulgen procedure. Chicken erythrocytes were used as test cells. Cytophotometric measurements were made using a Zeiss scanning stage cytophotometer coupled to a PDP 11/10 minicomputer using the BICOSCAN program to obtain values for protein per cell, protein per ‘nuclear area’ and DNA per nucleus. With 5N HCl as the Feulgen hydrolysis agent, DNFB staining, applied before the Feulgen procedure, was found to be unaffected by hydrolysis conditions required to give optimum Feulgen staining and showed only small losses after longer hydrolysis times. On the other hand measurements of NYS staining, of necessity applied after the Feulgen procedure, seem to be susceptible to the duration of Feulgen hydrolysis. This susceptibility is probably due to the interaction of the DNA phosphates with the basic amino acid residues, potential binding sites for NYS. Since the degree of this interaction may be variable, it is argued that NYS binding will measure the available basicity of proteins at the time of staining but no specific protein fraction. DNFB binding is unaffected by DNA-protein interactions and therefore can give a more reliable measure of ‘nuclear’ protein, particularly in conjunction with Feulgen-DNA measurements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493021
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  • 8
    Electronic Resource
    Electronic Resource
    Nuclear changes accompanying cell differentiation in stems of Pisum sativum L. (1982)
    Springer
    Histochemistry and cell biology 75 (1982), S. 327-340 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Nuclear DNA, nuclear protein and nuclear size have been measured in cells of the cortex, pith and vascular tissue from three successive internodes in the stem of Pisum sativum. New techniques of computer-linked cytophotometry were used to measure these parameters simultaneously in both section and squash preparations. In cortical cells no endoreduplicated nuclei were seen in the internodes measured. In cortical cells from the oldest internode measured, a population of large nuclei with the 2C DNA amount was observed which was not present in the younger internodes. In the oldest pith nuclei measured a few 8C nuclei were present, but maturing pith was most characterized by increasing nuclear size and the population of nuclei accumulating with the 4C DNA amount. Polyploid nuclei were present in all of the vascular tissue measured, including the youngest internode. Maturing vascular tissue was also characterized by increasing nuclear size. Nuclear protein measurements demonstrated a close link between nuclear protein and nuclear size and suggest that increased nuclear size, with constant DNA content, may be due to increased nuclear protein. This raises the question of the nature and function of this nuclear protein, perhaps more characteristic of differentiating cells than dividing cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00496736
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  • 9
    Electronic Resource
    Electronic Resource
    Nuclear and nucleolar protein during the cell cycle in differentiating Pisum sativum vascular tissue (1983)
    Springer
    Histochemistry and cell biology 79 (1983), S. 353-364 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Squash preparations of Pisum sativum fourth internode tissue were stained with a combined Feulgen and dinitrofluorobenzene (DNFB) procedure. Nuclei from differentiating xylem vessel elements, phloem sieve tube elements and phloem fibres were measured for their DNA and protein contents with a Zeiss scanning cytophotometer linked to an interactive computer system. Nuclei wer examined from both slowly growing and more rapidly growing internodes. A computer program was constructed to calculate nuclear protein alone as well as the ratio of DNFB (protein) to Feulgen (DNA) staining in each 0.5×0.5 μm measuring point. Nuclei were assigned to each of ten interphase fractions based on DNA content, nuclear area and percent condensed chromatin. There was a slight increase of nuclear protein during G1, a gradual increase in S, and a continued, often sharper, rise as G2 proceeded. In all three cell types, there was, on the average, a higher protein content throughout interphase in nuclei from the more rapidly growing internodes than from the slower growing ones. A population of fibre nuclei designated G0, however, differed from phloem and xylem G0 nuclei in the pattern of protein change. The nucleolar protein/DNA ratios of xylem nuclei increased in G1, showed no significant change in S, but increased thereafter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00491771
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  • 10
    Electronic Resource
    Electronic Resource
    Quantitative changes in the degree of chromatin condensation during the cell cycle in differentiating Pisum sativum vascular tissue (1983)
    Springer
    Histochemistry and cell biology 78 (1983), S. 101-109 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fixed tissue from the base of the fourth internode above the cotyledons of Pisum sativum plants were Feulgen-stained for DNA. In squash preparations three cell types: xylem vessel elements, phloem fibres and phloem sieve tube elements were identified. Measurement of Feulgen absorbance values in 0.5×0.5 μm measuring points across each nucleus were obtained with a scanning cytophotometer. Condensed chromatin was defined as any measuring point having an absorbance value greater than 0.32. Calculating ‘percent, condensed chromatin’ as the percentage of the total nuclear DNA contained in those densely stained points, the pattern of change in this parameter during interphase in each cell type was observed. A generally similar pattern occurs in all three cell types. Percent condensed chromatin decreased from about 45% to 12% during G1, increased rapidly to the end of S, decreased during G2 and the following G1 to increase again during the subsequent S phase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00491116
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