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  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 233 (1971), S. 491-492 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] There is little evidence that (d) and (e) are significant except possibly in isolated cases, whereas (c) is probably important only at the start of the corrosion process due to the eventual formation of protective sulphide films in the presence of free sulphide ion6. Much support for (a), the ...
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 30 (1958), S. 1-16 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 71 (1970), S. 214-219 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. Nine strains of Desulfovibrio, representing 4 species, grew by dismutation of malate in “sulphate-free” medium; succinate, fumarate and acetate were end-products of growth. 2. Two strains of D. vulgaris could only grow in malate medium in presence of sulphate as terminal electron acceptor. 3. Two dismuting strains of D. desulfuricans and two non-dismuting strains of D. vulgaris, all grown in a lactate medium, showed fumarase activity of the same order of magnitude. 4. The two dismuting strains showed high succinate dehydrogenase activity when grown in lactate medium; one of the non-dismuting strains (Woolwich) exhibited very low activity, while in the other non-dismuter (Hildenborough) the presence of succinate dehydrogenase was not conclusively proved.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 71 (1970), S. 196-198 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The abnormally high resistance of certain marine strains of the sulphate-reducing bacteria to the bacteriostatic effects of cetyltrimethylammonium bromide (CTAB) has been studied. A strain of the NaCl-requiring species Desulfovibrio salexigens and a halotolerant strain of D. desulfuricans both showed a marked increase in resistance to CTAB (about one-hundredfold for the former organism) with increasing salinity of the growth medium: this was attributed to enhanced micelle formation by CTAB in presence of NaCl.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 80 (1971), S. 324-337 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. Calculation showed that rates of sulphate reduction by the Hildenborough strain of Desulfovibrio vulgaris in non-nutrient buffer with gaseous hydrogen as electron donor were as high as, or higher than, the maximum possible rate of sulphate reduction during growth. 2. Respirometry showed that H2 oxidation was completely suppressed during growth unless the whole electron transport system was bypassed with a redox dye or the sulphate reductase system (terminal electron acceptor) was bypassed with FMN. 3. Growth experiments with intermittent sulphate feeds showed that cells under H2 “wasted” most of the sulphate by a reaction unconnected with growth, and thus made much less growth than did similar cultures under N2. 4. H2 exerted no discernible “lactate-sparing” action (that is, it did not serve as an alternative energy source) in low-lactate medium. The results are discussed in relation to metallic corrosion by the sulphate-reducing bacteria.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 28 (1957), S. 1-17 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 36 (1960), S. 219-228 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. Studies have been made on the nutritional characteristics of five members of the class Xanthophyceae: the coccoid forms Botrydiopsis intercedens, Bumilleriopsis brevis, Chlorellidium tetrabotrys and Polyedriella helvetica, and the filamentous form Tribonema minus. In addition, further studies on Monodus subterraneus and Tribonema aequale are reported. A wide range of nutritional types has been found in this class, from obligate phototrophs (Monodus and Polyedriella) to one that assimilated in the dark every organic substrate tested (Bumilleriopsis). The two species of Tribonema proved to be dissimilar with respect to their organic nutrition. Lag phases were exhibited by Bumilleriopsis before the commencement of chemo-organotrophic growth. 2. Good growth of all the above species, except Chlorellidium, which was not examined, was obtained in Miller and Fogg's (1957) standard mineral medium for Monodus. Bumilleriopsis brevis had the highest relative growth constant (0.88 log10/day units at 25°C) and reached the highest cell concentration (978,000 cells/mm3). 3. The obligate phototrophs showed a capacity to remain viable in the dark in mineral medium for long periods (60 days or more) whereas, with the exception of Bumilleriopsis, the facultative chemo-organotrophs remained viable for less than 18 days. 4. Monodus subterraneus, after dark incubation, exhibited a lag period when subcultured before growth began. This phenomenon has been shown to be due to the progressive death of cells in the dark rather than to an adaptation phase.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 43 (1975), S. 171-187 
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The growth of the Hildenborough strain ofDesulfovibrio vulgaris has been studied in stirred cultures flushed with Ar/CO2 (95 + 5) and H2/CO2 (95 + 5). Sodium lactate was used as the carbon-energy source with sodium sulphate as the terminal electron acceptor. The initial lactate concentration was varied in defined media (pH 7) augmented with 0.5 mM citrate as chelating agent, and growth was followed to completion. Yields were calculated for the region of strict lactate limitation,i.e. where the total growth was directly proportional to the initial lactate concentration. The maximum specific growth rate was identical for both gases (0.15 h−1); however, the yield under hydrogen was 25.8±2.1 g/mole of lactate compared with 9.20±0.78 g/mole under argon (95% confidence limits). No growth occurred in the absence of sodium lactate. The total protein contents of the cells were similar in both cases (67–75 per cent). In addition the organism was grown under a hydrogen atmosphere in medium augmented with 0.01 per cent yeast extract. This concentration of yeast extract was estimated to provide a sufficient exogenous supply of amino acids for cell biosynthesis at the expected levels of cell density. Although a significant increase in the growth rate was observed (0.20 h−1) the yield was only marginally improved (28.2±2.7 g/mole) In the absence of lactate the residual growth (7 mg/l) was explained by the utilisation of substances in the yeast extract as energy source. Isobutanol was tested as an energy source under both nitrogen and hydrogen in media augmented with 0.02 per cent yeast extract. The marginal growth that occurred was again explained by the utilisation of some component of the yeast extract as energy source.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 43 (1975), S. 171-187 
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The growth of the Hildenborough strain ofDesulfovibrio vulgaris has been studied in stirred cultures flushed with Ar/CO2 (95 + 5) and H2/CO2 (95 + 5). Sodium lactate was used as the carbon-energy source with sodium sulphate as the terminal electron acceptor. The initial lactate concentration was varied in defined media (pH 7) augmented with 0.5 mM citrate as chelating agent, and growth was followed to completion. Yields were calculated for the region of strict lactate limitation,i.e. where the total growth was directly proportional to the initial lactate concentration. The maximum specific growth rate was identical for both gases (0.15 h−1); however, the yield under hydrogen was 25.8±2.1 g/mole of lactate compared with 9.20±0.78 g/mole under argon (95% confidence limits). No growth occurred in the absence of sodium lactate. The total protein contents of the cells were similar in both cases (67–75 per cent). In addition the organism was grown under a hydrogen atmosphere in medium augmented with 0.01 per cent yeast extract. This concentration of yeast extract was estimated to provide a sufficient exogenous supply of amino acids for cell biosynthesis at the expected levels of cell density. Although a significant increase in the growth rate was observed (0.20 h−1) the yield was only marginally improved (28.2±2.7 g/mole) In the absence of lactate the residual growth (7 mg/l) was explained by the utilisation of substances in the yeast extract as energy source. Isobutanol was tested as an energy source under both nitrogen and hydrogen in media augmented with 0.02 per cent yeast extract. The marginal growth that occurred was again explained by the utilisation of some component of the yeast extract as energy source.
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 59-76 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An apparatus is described in which large numbers of algal cultures can be maintained under the following conditions: (1) Constant temperature within the range 5 to 70°C, four different temperatures being maintained simultaneously; (2) uniform illumination at intensities up to 20,000 metre-candles; (3) continuous shaking at variable speed; and (4) aeration under aseptic conditions with mixtures of up to four different gases in any desired proportions.Among other features, thermoregulator heads, flow-meters and heaters for sterile filters, of improved design are described.To illustrate the use of the apparatus, data showing the effects of light intensity and temperature on the growth of Monodus subterraneus are presented.
    Additional Material: 8 Ill.
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