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  • 1
    Electronic Resource
    Electronic Resource
    Analogs of insulin. I. Synthesis of destripeptide B28-30 bovine insulin and destripeptide B28-30 procine (human) insulin (1971)
    s.l. : American Chemical Society
    Biochemistry 10 (1971), S. 3884-3889 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00797a014
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  • 2
    Electronic Resource
    Electronic Resource
    Flextensional Barium Strontium Titanate Actuators (2003)
    Westerville, Ohio : American Ceramics Society
    Journal of the American Ceramic Society 86 (2003), S. 0 
    Details
    ISSN: 1551-2916
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: This paper describes the performance of the cymbal flextensional transducer using Dy-doped barium strontium titanate (BST) as the driver material. BST was first characterized for its dielectric and loss behavior as a function of temperature and electrical bias field. With no electrical bias, the transition temperature was measured to be near 20°C and have a dielectric constant 〉20 000. The strain of a BST disk was then measured and compared with other ceramics. At room temperature the strain and average effective piezoelectric d33 of this non-lead composition was slightly larger than Navy type I lead zirconate titanate (PZT-4) ceramic. The strain/field behavior was also measured as a function of temperature. Cymbal capped BST ceramic was found to have an amplified displacement of 28×, also very similar to type I ceramic. The stiffness of BST was found to be tunable by dc voltage and 2 to 3 times larger than that of PZT. This material has promise for applications in actuators and transducers with large generative force.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1151-2916.2003.tb03400.x
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  • 3
    Electronic Resource
    Electronic Resource
    Stereochemical consequences of halogen atom substitution. 1. Rotational conformer effects in gaseous diastereomeric 2,3-dihalobutanes (1990)
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 94 (1990), S. 2316-2320 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/j100369a023
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  • 4
    Electronic Resource
    Electronic Resource
    The origin of greater-than-unit-length plasmids generated during bacterial conjugation (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 7 (1993), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: In Gram-negative bacteria, the general mechanism of conjugal plasmid transfer, which is probably similar for many different groups of plasmids, involves the transfer of a single plasmid DNA strand with 5′ to 3′ polarity. Transfer is initiated by nicking of the duplex DNA at a particular site, i.e. the origin of transfer (oriT). We constructed plasmids containing two directly repeated copies of oriT, derived from the broad-host-range plasmid R1162 and flanking the lac operator. The number of lacO copies in the plasmid after transfer could be determined from the colour of transconjugant colonies on medium containing X-Gal. When the oriTs were mutated to prevent initiation and termination of transfer at the same oriTs, almost all of the transconjugant cells contained greater-than-unit-length plasmids with two copies of lacO and three copies of oriT. We show that these molecules were generated by an intramolecular, conjugation dependent mechanism unlikely to depend solely on a pre-existing population of circular or linear multimers in donor cells. We propose that the greater-than-unit-length molecules were instead generated by a rolling-circle mechanism of DNA replication.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1993.tb01120.x
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  • 5
    Electronic Resource
    Electronic Resource
    Localized denaturation of oriT DNA within relaxosomes of the broad-host-range plasmid R1162 (1995)
    Osney Mead, Oxford OX2 0EL, UK : Blackwell Scientific Publication
    Molecular microbiology 17 (1995), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The broad-host-range, multicopy plasmid R1162 is efficiently mobilized during conjugation by the self-transmissible plasmid R751. The relaxosome, a complex of plasmid DNA and R1162-encoded proteins, forms at the origin of transfer (oriT) and is required for mobilization. Transfer is initiated by strand- and site-specific nicking of the DNA within this structure. We show by probing with potassium permanganate that oriT DNA is locally melted within the relaxosome, in the region from the inverted repeat to the site that is nicked. Mutations in this region of oriT, and in genes encoding the protein components of the relaxosome, affect both nicking and melting of the DNA. The nicking protein in the relaxosome is MobA, which also ligates the transferred linear, single strand at the termination of a round of transfer. We propose that there is an underlying similarity in the substrates for these two MobA-dependent, DNA-processing reactions. We also show that MobA has an additional role in transfer, beyond the nicking and resealing of oriT DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1995.mmi_17040727.x
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  • 6
    Electronic Resource
    Electronic Resource
    Selection of plasmid molecules for conjugative transfer and replacement strand synthesis in the donor (2002)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 46 (2002), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Plasmid selection and strand replacement synthesis in donor cells during conjugative transfer was examined by a procedure involving electroporation of test plasmid DNA, containing a base pair mismatch, into donor cells prior to mating. Multiple copies of the plasmid were transferred from a donor cell that allowed vegetative replication of the plasmid. Under conditions non-permissive for vegetative replication, there were further rounds of transfer after a lag period. Strand replacement in the donor did not depend solely on the initiation mechanism for vegetative replication, indicating a conjugation-specific mechanism was also available. The lag period between first and second rounds of transfer argues against the transfer of multiple copies into recipients by the spooling of copies generated on a master molecule by rolling-circle replication.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.2002.03195.x
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  • 7
    Electronic Resource
    Electronic Resource
    The 20 bp, directly repeated DNA sequence of broad host range plasmid R1162 exerts incompatibility in vivo and inhibits R1162 DNA replication in vitro (1987)
    Springer
    Molecular genetics and genomics 208 (1987), S. 390-397 
    Details
    ISSN: 1617-4623
    Keywords: Incompatibility ; Direct repeats ; DNA replication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The broad host range plasmid R1162 contains a directly repeated, 20 bp DNA sequence in the region of the plasmid required in cis for replication and maintenance. This sequence has been chemically synthesized and cloned, and shown to be sufficient for expression of plasmid incompatibility. The sequence also inhibits replication of R1162 DNA in a cell-free system. The strengths of both these effects are determined by the number of direct repeats (DRs) present, and are also affected to similar degrees by different mutations within the repeated sequence. Several of the mutations were tested for their effect in cis on plasmid maintenance in the cell, and one was found to cause an increase in plasmid copy number. The results suggest that the direct repeats exert incompatibility by inhibiting DNA replication, presumably because they are the binding sites for a limiting essential protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00328129
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  • 8
    Electronic Resource
    Electronic Resource
    Nucleotide sequence and functional properties of DNA encoding incompatibility in the broad host-range plasmid R1162 (1984)
    Springer
    Molecular genetics and genomics 194 (1984), S. 423-431 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 370 base pair (bp) fragment of R1162 DNA encoding the incompatibility determinant has been cloned and sequenced. The DNA is located between 6.1 and 6.5 on the R1162 map, near the origin of replication. The sequence contains three perfectly conserved 20 bp direct repeats, with 11 bp of this sequence repeated a fourth time. The direct repeat unit shows some homology with that of another, unrelated broad host-range plasmid, RK2. The cloned DNA has two other properties: it lowers the copy number of R1162 when cloned into this plasmid, and it is required in cis for replication of R1162 satellite plasmids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425554
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  • 9
    Electronic Resource
    Electronic Resource
    Integrative suppression of dnaA46 by broad host-range plasmid R1162 (1988)
    Springer
    Molecular genetics and genomics 215 (1988), S. 139-145 
    Details
    ISSN: 1617-4623
    Keywords: R1162 ; dnaA ; Integrative suppression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Replication of plasmid R1162 DNA does not require the product of the dnaA gene. An integrated copy of the plasmid can suppress the temperature-sensitive dnaA46 allele when (1) additional plasmid copies are present in the cytoplasm and (2) an inactive replication origin, generated by deletion, is also present in the chromosome. We propose that the inactive origin sets the rate of initiation of chromosome replication at a level compatible with cell viability, possibly by providing additional binding sites for an R1162-encoded protein that is rate-limiting for plasmid replication.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331315
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  • 10
    Electronic Resource
    Electronic Resource
    Expression of incompatibility by derivatives of the broad host-range inc P-1 plasmid RK2 (1979)
    Springer
    Molecular genetics and genomics 177 (1979), S. 155-161 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A segment of DNA encoding incompatibility on the inc P-1 plasmid pRK248 was identified by the analysis of deletions generated in vitro, and then cloned into several unrelated and mutually compatible plasmids. These derivatives were tested for expression of P-1 incompatibility. It was demonstrated by transformation experiments that P-1 plasmids were efficiently eliminated from an E. coli host following introduction of any one of the derivatives. However, all the derivatives were compatible with each other. The cloned segment of pRK248 DNA is itself capable of autonomous replication, without being cloned into any plasmid, if plasmid-specified gene products are provided in trans. This satellite plasmid is eliminated from the cell by the inc P-1 plasmid pRK286. The results argue against a partitioning mechanism as the basis for P-1 incompatibility but are consistent with incompatibility being the consequence of negative regulation of copy number. For the inc P-1 system, susceptibility of the plasmid to elimination, but not its ability to eliminate, requires that the P-1 replication system is active.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267265
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