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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 115 (1996), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Racial evolution of sunflower broomrape (Orobanche cernua) has been very rapid in Spain during recent years, in which resistance has been overcome several times and there has been an important increase in areas infested with this parasitic angiosperm. In order to find resistance to a highly virulent population of sunflower broomrape that could be used directly in breeding programmes, three different sets of cultivated plant material composed of 429 entries were tested by artificial inoculation. All evaluated inbred lines from Moden, Canada, were fully susceptible. Out of the 240 P.I. accessions tested, only 10 segregated for resistance to broomrape, the rest being susceptible. From the 160 USDA breeding lines evaluated, 5% were resistant and 19% segregated for resistance to O. cernua. These lines traced back mainly to crosses of RHA 274 and RHA 801 with Russian, Turkish and Romanian hybrids. The origin of P.I. accessions that segregated for resistance were primarily derived from the former USSR and from Romania.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 121 (2002), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Sunflower lines RHA-274, HA-61 and RHA-325 were studied for their resistance to race 330 of downy mildew (Plasmopara halstedii). The same inbred line, with normal (HA-89) or sterile cytoplasm (cmsHA-89) was used in all the crosses as susceptible parent, and, in each cross, only one genotype of the resistant parent was studied. The resistant-to-susceptible ratios obtained in the BC1 and F2 progenies from the crosses of the lines RHA-274 and HA-61 to cmsHA-89 and HA-89, respectively, suggested that, in each resistant line, two dominant genes are responsible for resistance to this downy mildew race. One of the genes (A) is epistatic to the other (B), and the recessive allele b in homozygosity is also epistatic to aa, with plants carrying aabb genotypes being resistant. Resistance to race 330 seemed to be controlled by two complementary genes in the sunflower inbred line RHA-325, the dominant allele of one of them being present in cmsHA-89. In the genotypes HA-89 or cmsHA-89, the existence of genes that modify the expected segregations following the crosses with resistant parents is proposed. It is concluded that, although major genes have been described as responsible for monogenic resistance to downy mildew, other types of regulation of this character, such as complementarity and epistatic relationships, do occur.
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  • 3
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Broomrape (Orobanche cernua Loelf.) is a sunflower parasite which has become a potential threat to this crop in Spam with an increasing incidence in recent last years. In order to investigate the racial situation of the parasite in Spam and to evaluate the resistance of differentials and inbred lines currently used in breeding programmes, two experiments were carried out. In the first, two sets of European sunflower differentials were inoculated with three broomrape populations from different geographical origin. The second experiment studied the reaction to the parasite of 38 inbred lines in comparison with resistant and susceptible checks. Reactions of differential lines resistant to known European broomrape races ranged from complete immunity of lines P-1380-2A and ‘Jdanovski 8281’ to complete susceptibility of ‘Record’ and the universal suscepts. These results confirmed the existence of several physiological races of broomrape in Spain and a different racial composition from that of Eastern Europe. Results from the second experiment showed good resistance of half the restorer lines tested and 36 % of the maintainers and cms lines. This material together with the resistant differential lines constitutes good starting material to be used in developing cultivars resistant to broomrape populations in Spain.
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  • 4
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A new race F of broomrape overcomes all known resistance genes in cultivated sunflower, but recently, sources of resistance against race F have been developed. The objective of the present research was to study the inheritance of resistance to race F in crosses between 12 resistant sunflower breeding lines, derived from three different sources of resistance, and the susceptible male-sterile line P-21. Parental lines and F1, F2, F3 and BC1 generations were evaluated for broomrape resistance. Segregations in the F2 and BC1 to resistant parent approached resistant to susceptible ratios of 1: 15 and 1: 3, respectively, in most of the crosses, suggesting a double dominant epistasis. However, segregations of 3: 13 and 1: 1 for F2 and BC1, respectively, indicating a dominant-recessive epistasis, were also found. The F3 data confirmed these results. Owing to the recessive nature of this resistance, it must be incorporated into both parental lines for developing resistant hybrid cultivars.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 117 (1998), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Interspecific hybrids and backcross generations between the wild perennial species Helianthus resinosus, Helianthus paucifiorus, Helianthus laevigatus, Helianthus nuttallii ssp. nuttallii T. & G. and Helianthus giganteus, resistant to broomrape (Orobanche cernua) and susceptible inbred lines were obtained to study crossability to cultivated sunflower and the transmission and expression of resistance to this parasitic weed. Conventional crosses with all the species tested were successful except for the crosses with diploid H. giganteus, for which embryo rescue techniques were needed to overcome hybrid incompatibility. Pollen viability and seed set were highest for F1 hybrids with hexaploid species and lowest for those with the diploid H. giganteus. We evaluated F1, BC1F1, some BC2F1 plants and the wild and cultivated parents. The wild species and interspecific hybrids were resistant to broomrape infection except for H. nuttallii, which showed segregation, indicating that the resistance is dominant. The crossability and resistance of F1, and back-cross generations of species with different ploidy levels indicate that the transfer of broomrape resistance to cultivated sunflower is feasible.
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  • 6
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effects of pre-planting solarization or fumigation with metham-sodium of sand-mulched soil on fusarium wilt of watermelon in plastic house culture were investigated at Almeria, south-eastern Spain. In two trials, 2 months' solarization increased the average maximum soil temperature by c. 5°C to 44-48° C at 10 cm depth and by 4-5° C to 40-42° C at 20-30 cm. The amount of Fusarium oxysporum in the upper 15 cm of a naturally infested soil was reduced by solarization and by fumigation. During the 9 months following treatment, the F. oxysporum population stabilized at a low level in soil solarized for 2 months, but fluctuated in soil solarized for 1 month and increased in fumigated soil. The amount of wilt in watermelon sown into this soil after treatment was generally low; plants growing in solarized or fumigated soil suffered less wilt than plants in untreated soil but the differences were not significant. In a soil artificially infested with the highly pathogenic race 2 of F. oxysporum f. sp. niveum, F. oxysporum populations were greatly reduced following solarization or fumigation, and fluctuated erratically thereafter. Solarization for 2 months completely controlled wilt in watermelon and gave a fruit yield almost five times that of plants in untreated soil. Solarization for 1 month only slowed disease development slightly but gave a yield more than twice that in untreated soil. Fumigation with metham-sodium retarded disease development considerably and tripled fruit yield. Plant performance was significantly better in soil solarized for 2 months than in uninfested control soil, suggesting beneficial effects of this treatment additional to wilt control.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Publishing Ltd
    Plant pathology 48 (1999), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Surveys between 1989 and 1993 in the major garlic production areas of Spain identified a new leaf spot disease, characterized by white and purple lesions followed by extensive necrosis. Isolation and pathogenicity tests with fungal isolates taken from these spots indicated that Stemphylium vesicarium was the causal agent. Pseudothecia of the teleomorph stage, Pleospora sp., were found on leaf debris from affected plants. Inoculation of garlic and onion plants with residues carrying mature pseudothecia, or with ascospore suspensions obtained from the pseudothecia, resulted in the development of white and purple leaf spots. Wetness periods longer than 24 h were required for symptom development under controlled conditions. Isolates of S. vesicarium from garlic, onion and asparagus caused disease in all three hosts. In garlic, cv. Blanco de Vallelado was most susceptible, while lines B4P17 and B6P1, and cvs Iberose and Golourose were less susceptible to the disease.
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
    Plant pathology 46 (1997), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Experiments were conducted in the Guadalquivir Valley of Andalucía, southern Spain, in 1986 and 1987, using field plots naturally infested with different inoculum densities of the defoliating and nondefoliating pathotypes of Verticillium dahliae to determine the influence of verticillium wilt epidemics on yield of cotton cultivar Coker 310. The total number of bolls, the number of open bolls, and seed cotton yield were related to the growth stage of plants at first appearance of foliar symptoms, and to inoculum density and virulence of the V. dahliae pathotype prevailing in the soil. For the three yield components, the greatest reduction was observed in plants showing symptoms before opening of first flowers (about 650 degree-days after sowing). Yield increased with delay in the development of foliar symptoms during the crop season, and the effect of the wilt epidemics on yield was small or nil for plants that developed symptoms after opening of the first bolls (1400–1500 degree-days after sowing). A multiple regression equation was derived that related yield reduction to the physiological time accumulated from the time of sowing until the appearance of foliar symptoms and to the rate of disease intensity increase over physiological time. This multiple point model explained about 70% of the variation in cotton yield loss due to verticillium wilt.
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  • 9
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Phytophthora root rot is of paramount importance in avocado orchards of southern Spain. Soil solarization has been demonstrated to control the pathogen in infested areas from which infected trees had been removed. We aimed to determine whether soil solarization in established avocado orchards controls the disease. Soil solarization increased average maximum hourly soil temperatures by 6.5–6.9°C in unshaded areas of avocado orchards in coastal areas of southern Spain, depending on depth and year. The corresponding temperatures in shaded areas were c. 2–3°C lower. P. cinnamomi in soil, on infected avocado rootlets, and in a nutrient substrate buried at 30–60 cm depth was reduced to negligible amounts after 6–8 weeks of solarization in both unshaded and shaded locations of avocado orchards. P. cinnamomi could not be detected in avocado rootlets up to 14 months later, suggesting a long-term effect. Soil solarization did not affect growth of the trees, and fruit yields were increased as compared with control plots. Following soil solarization for 3 weeks from mid-July 1994, when maximum hourly temperatures reached 33–36°C, P. cinnamomi could not be recovered from a depth of up to 45 cm in unshaded areas or from a depth of up to 30 cm in shaded areas after the initial 10-day period. The viability of inoculum of the pathogen buried at depths between 15 and 60 cm in bare soil was determined by sequential sampling in two solarization experiments starting 12 June and 4 July 1995, respectively. In the first experiment, P. cinnamomi could not be detected at any depth after 4–8 weeks of solarization in unshaded areas but could be recovered at all depths except 15 cm in shaded areas. In the second experiment, where temperatures were higher and the soil surface not shaded, P. cinnamomi could not be recovered after 2 weeks at 15 and 30 cm.
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  • 10
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Eight field experiments (I-VIII) were conducted in clay soils naturally infested with a cotton-defoliating pathotype of Verticillium dahliae in the lower Guadalquivir Valley of Andalucia, southern Spain, during the period 1986-90. Experiments I-VI aimed to determine the efficacy of soil solarization in reducing populations of the pathogen in soil and eventually contributing to the control of Verticillium wilt of cotton. The population of V. dahliae in the 0-to 40-cm soil layer was reduced to undetectable or very low levels after solarization for 6 to 10 weeks. The final incidence of Verticillium wilt in the cotton crop following solarization was reduced to 13% or less in solarized plots, compared to 55–90·5% in unsolarized controls. The onset of disease incidence in the solarized plots was delayed by 2–7 weeks, increased at a lower rate, and had a smaller area under the disease progress curve, compared to that in unsolarized plots. Seed cotton yields in solarized plots increased by 11·3-130·9% depending upon experiments, cultivars and years. Experiments VII and VIII aimed to determine the use of the highly wilt-tolerant cotton cv. Acala GC 510 for control of the disease that had been cropped to susceptible cotton cultivars the year before in solarized soils. Solarized or unsolarized plots were first sown to susceptible cotton cvs Acala SJ2 and Coker 310, and the following year were sown to cv. Acala GC 510. The inoculum density of V. dahliae at the time of sowing cv. Acala GC 510 in previously solarized plots had increased to moderate levels, but remained considerably lower than that in unsolarized plots. The final disease incidence in cv. Acala GC 510 grown in unsolarized plots was lower than that in susceptible cultivars grown in the same plots the year before. Furthermore, the disease incidence in cv. Acala GC 510 grown in solarized plots was as high as that in susceptible cultivars grown the year before with much less initial inoculum.
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