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  • 1
    Electronic Resource
    Electronic Resource
    Verification of the introgression of Erianthus arundinaceus germplasm into sugarcane using molecular markers (2005)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 124 (2005), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Erianthus arundinaceus has a number of important agronomic traits including good ratooning ability, tolerance to both drought and water logging, disease resistance and vigour and is of interest as a potential source of parental germplasm to sugarcane breeders. However, to date, attempts to produce fertile hybrids between sugarcane (Saccharum spp.) and E. arundinaceus have been unsuccessful. Microsatellite markers that generated genus-specific markers and were highly polymorphic within sugarcane were identified. The microsatellite markers and 5S rDNA PCR were used to screen intergeneric (F1) clones from Saccharum officinarum×E. arundinaceus crosses, and two Saccharum backcross progeny (BC1) populations derived from crosses between selected F1 clones and sugarcane (Saccharum spp.), to identify genuine Saccharum spp. BC1 progeny. The 5S rDNA PCR marker and highly polymorphic microsatellites with Erianthus-specific bands confirmed the F1 parent of the two putative BC1 populations was a S. officinarum×E. arundinaceus hybrid and allowed the identification of the genuine BC1 progeny from selfs of the F1 parent. This is the first verification of BC1 progeny from an F1 intergeneric hybrid x sugarcane (Saccharum spp.) clone with molecular markers and confirms the introgression of E. arundinaceus germplasm into sugarcane. It should now be possible to exploit genes of value from E. arundinaceus in sugarcane breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2005.01099.x
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  • 2
    Electronic Resource
    Electronic Resource
    Relationships between hard-seededness and seed weight in mungbean (Vigna radiata) assessed by QTL analysis (2005)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 124 (2005), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Weather damage reduces the value of commercial mungbean. but hard-seededness can reduce the level of damage. However. attempts lo breed large- and hard-seeded mungbean varieties have been unsuccessful. To understand the relationship between seed weight and hard-seededness. these trails were investigated using a quantitative trail loci (QTL) mapping approach with a recombinant inbred population derived from a cross between a completely soft-seeded variety and a completely hard-seeded genotype. The two parental genotypes also had a sixfold difference in seed weight. QTL analyses revealed four loci for hard-seededness and 11 loci for seed weight. Two of the hard-seeded ness loci co-localized with seed weight QTL. When seed weight was used as a covariate in the analysis of hard-seededness from the field data, two of the four hard-seeded QTL remained significant with the effect al one of these remaining unchanged. These results explain why retaining hard-seededness in large seeded mungbean lines has been unsuccessful. The existence of a persistent locus, however. indicated that breeding large and persistently hard-seeded varieties of mungbean may be possible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2005.01084.x
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of phylogenetic relationships in the Triticeae tribe using RFLPs (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 649-655 
    Details
    ISSN: 1432-2242
    Keywords: Cladogram ; Phenogram ; Phylogenetic tree ; Restriction fragment length polymorphisms ; Triticeae tribe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The use of restriction fragment length polymorphisms in combination with other approaches is very useful for the reconstruction of evolutionary events revealing phylogenetic relationships. A set of 21 cDNA probes hybridizing to different chromosome arms in hexaploid wheat was used in a series of experiments designed to estimate the phylogenetic relationships among and within 16 species of the Triticeae tribe. A high degree of polymorphism was found both between and within the species examined. The RFLP data were used to generate a cladogram and a phenogram in order to compare the two different methods of constructing phylogenetic trees. The results of both methods were consistent with each other and with the general taxonomic information provided by earlier morphological studies, meiotic pairing analysis, isozyme tests, and sequence alignment in theTer,NOR, and5s DNA loci. In addition, several correlations were found between the geographical origin of accessions from the same species and their phylogenetic relationships as shown by the cladogram and phenogram.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00838722
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  • 4
    Electronic Resource
    Electronic Resource
    Ribosomal DNA variations in Erianthus, a wild sugarcane relative (Andropogoneae-Saccharinae) (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 733-743 
    Details
    ISSN: 1432-2242
    Keywords: Key words rDNA ; 5S DNA ; RFLP ; Erianthus sect. Ripidium ; Saccharum complex ; Germplasm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Variation at and 5S ribosomal DNA loci was assessed on 62 Erianthus Michx. clones, representing 11 species, and 15 clones from two Saccharum L. species used as a reference. Genus-specific markers for Erianthus Michx. sect. Ripidium Henrard (Old World species) were identified. Ribosomal DNA units in Erianthus sect. Ripidium exhibited an additional BamHI site compared to Saccharum, and 5S units showed length and restriction-site differences between Erianthus and Saccharum. These markers will be useful to follow introgression in SaccharumErianthus hybrids. Six ribosomal units (genes) were revealed in Erianthus sect. Ripidium, differing by restriction-site positions and/or length. These results provided new information on species relationships and evolution within the genus Erianthus. The Indonesian and Indian forms of E. arundinaceus (Retz.) Jeswiet gave different restriction patterns, which were similar to those of E. bengalense (Retz.) R. C. Bharadwaja and E. procerus (Roxb.) Raizade, respectively. The two species, E. ele-phantinus Hook.f. and E. ravennae (L.) P. Beauv., could also be differentiated at this locus. Two of the New World Erianthus species studied, E. rufipilus (Steud.) Griseb. and E. longisetosus Andersson, appeared more like Erianthus sect. Ripidium, whereas E. trinii Hack. and E. brevibardis Michx. showed patterns consistent with Miscanthus sinensis Andersson and S. spontaneum L., respectively. Finally, the comparison of rDNA restriction maps among Erianthus sect. Ripidium, Saccharum, sorghum and maize, led to unexpected conclusions concerning the relationships between the different genera and the position of Erianthus in the “Saccharum complex”.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050186
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  • 5
    Electronic Resource
    Electronic Resource
    Identification of genomic regions associated with stay green in sorghum by testing RILs in multiple environments (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 1225-1232 
    Details
    ISSN: 1432-2242
    Keywords: Key words Sorghum ; Genome mapping ; Stay green ; Marker-assisted selection ; Multi-environment testing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Stay green is an important drought resistance trait for sorghum production. QTLs for this trait with consistent effects across a set of environments would increase the efficiency of selection because of its relatively low heritability. One hundred and sixty recombinant inbreds, derived from a cross between QL39 and QL41, were used as a segregating population for genome mapping and stay green evaluation. Phenotypic data were collected in replicated field trials from five sites and in three growing seasons, and analysed by fitting appropriate models to account for spatial variability and to describe the genotype by environment interaction. Interval mapping and non-parametric mapping identified three regions, each in a separate linkage group, associated with stay green in more than one trial, and two regions in single trial. The regions on linkage groups B and I were both consistently identified from three trials. The multiple environment testing was very helpful for correctly identifying QTLs associated with the trait. The utilisation of molecular markers for stay green in sorghum breeding is also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051428
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  • 6
    Electronic Resource
    Electronic Resource
    Ribosomal DNA variations in Erianthus, a wild sugarcane relative (Andropogoneae-Saccharinae) (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 733-743 
    Details
    ISSN: 1432-2242
    Keywords: rDNA ; 5S DNA ; RFLP ; Erianthus sect. Ripidium ; Saccharum complex ; Germplasm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Variation at the 18S+26S and 5S ribosomal DNA loci was assessed on 62 Erianthus Michx. clones, representing 11 species, and 15 clones from two Saccharum L. species used as a reference. Genus-specific markers for Erianthus Michx. sect. Ripidium Henrard (Old World species) were identified. Ribosomal DNA units in Erianthus sect. Ripidium exhibited an additional BamHI site compared to Saccharum, and 5S units showed length and restriction-site differences between Erianthus and Saccharum. These markers will be useful to follow introgression in Saccharum x Erianthus hybrids. Six ribosomal units (for 18+26S genes) were revealed in Erianthus sect. Ripidium, differing by restriction-site positions and/or length. These results provided new information on species relationships and evolution within the genus Erianthus. The Indonesian and Indian forms of E. arundinaceus (Retz.) Jeswiet gave different restriction patterns, which were similar to those of E. bengalense (Retz.) R. C. Bharadwaja and E. procerus (Roxb.) Raizade, respectively. The two 2n=20 species, E. ele-phantinus Hook.f. and E. ravennae (L.) P. Beauv., could also be differentiated at this locus. Two of the New World Erianthus species studied, E. rufipilus (Steud.) Griseb. and E. longisetosus Andersson, appeared more like Erianthus sect. Ripidium, whereas E. trinii Hack, and E. brevibardis Michx. showed patterns consistent with Miscanthus sinensis Andersson and S. spontaneum L., respectively. Finally, the comparison of rDNA restriction maps among Erianthus sect. Ripidium, Saccharum, sorghum and maize, led to unexpected conclusions concerning the relationships between the different genera and the position of Erianthus in the “Saccharum complex”.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226096
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  • 7
    Electronic Resource
    Electronic Resource
    DNA sequence analyses of the ribosomal spacer regions in theTriticeae (1988)
    Springer
    Plant systematics and evolution 160 (1988), S. 91-104 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Triticeae ; Ribosomal DNA spacer regions ; phenetic and molecular relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two regions of the ribosomal DNA (rDNA) were sequenced from a range of species from the tribeTriticeae. One region, the central spacer, was found to be more divergent in sequence than the other, the 18 S-spacer junction. Both regions contained sequences 20–30 bp long which were more highly conserved than the remainder of the region and their possible significance in rDNA expression is discussed. Phenetic relationships based on the sequence data were generally consistent with the relationships based on other criteria. Species possessing the S, E, J1J2, D, and B genomes clustered together, with the H genome species being the most distinct of those examined. The R, P, and V genome species occupy an intermediate position in the overall pattern of relationships. Some relationships differed in detail from those established by other parameters, for example the position of the N genome species, and explanations for discrepancies of this type are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00936712
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  • 8
    Electronic Resource
    Electronic Resource
    Variation at isozyme loci inTriticeae (1988)
    Springer
    Plant systematics and evolution 160 (1988), S. 123-142 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Triticeae ; Evolution ; isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An electrophoretic comparison of variation at 16 presumptive isozyme gene loci was performed for 17 species from the tribeTriticeae. Included in the analysis were annuals and perennials, and self- and cross-pollinating species, representing the H, I, P, N, R, V, S, E, J, J1J2, A, B, and D genomes. Perennial species were found to contain a significantly (marginally, at the 5% level) higher proportion of polymorphic loci and level of heterozygosity, than annual species. There were no significant differences between self- and crosspollinating species. Across all species, mean heterozygosity levels ranged from 0–0.225 and the % polymorphic loci from 6.3–56.3%. Genetic distance estimates varied from 0.08–0.39 for congeneric species. Relationships were deduced between the 17 species using phenetic and cladistic analyses and compared with relationships inferred from other parameters such as morphology and nucleotide sequence data. In general, the trees derived from the various relationships were concordant; the evolutionary basis for minor discrepancies between trees is also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00936714
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  • 9
    Electronic Resource
    Electronic Resource
    Amplification and dispersion of repeated DNA sequences in theTriticeae (1988)
    Springer
    Plant systematics and evolution 160 (1988), S. 39-59 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Triticeae ; Evolution ; dispersed repeated sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four representatives of a family of dispersed repetitive sequences which were prominent and dispersed in the E genome ofThinopyrum elongatum but poorly represented in wheat, were studied in detail. The 1.4kb sequences were present both as part of tandem and more complex arrays and appeared to have resulted from repeated amplification of the sequence and their dispersion throughout the genome. Subcloning of sections of the 1.4 kb sequences resulted in probes which improved the resolution of the E genome from the genomes in wheat and enabled identification of single E genome chromosomes introduced into wheat. The generality of these types of sequences in the tribeTriticeae was confirmed by isolating analogous sequences from the R (rye,Secale cereale), V (Dasypyrum villosum), and N (Psathyrostachys juncea) genomes. — The cloned repetitive sequences from the R, V, and N genomes each showed characteristic fluctuations in amount within the grasses examined in addition to being virtually absent from wheat. It is thus possible that these sequences may provide useful taxonomic indicators for establishing relationships within theTriticeae, as well as valuable probes for tracing alien chromatin introduced into wheat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00936708
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  • 10
    Electronic Resource
    Electronic Resource
    Evolution and systematic relationships in theTriticeae (Poaceae) (1988)
    Springer
    Plant systematics and evolution 160 (1988), S. 1-28 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Triticeae ; Systematics ; phylogeny ; cladistic ; phytogeography ; isozyme analyses ; chromosome pairing ; DNA analyses ; relationships ; molecular evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The evolution and taxonomic relationships in theTriticeae are discussed with the view to highlight aspects of this agronomically important group of plants, which may be of interest to molecular biology. Some of these aspects are addressed in more detail in adjoining papers in which specific genomic loci have been examined at the DNA sequence or isozyme level. Aspects discussed include the systematics and geographic distribution of theTriticeae species, isozyme and chromosome pairing studies on some of the species as well as more recent developments in DNA analyses. A survey of the systematics of theTriticeae indicated that the genomic system ofLöve is probably the most useful starting point for interpreting molecular data even though the system has many problems from a taxonomic point of view. The geographical distribution ofTriticeae species, using both published and unpublished data, suggested that information of this type taken together with the theory of continental drift provides a broad time-span for considering data from DNA sequence studies. The significance, and modes of analyses, of isozyme studies were assessed because they often provide valuable characters in determining relationships between species. The main character underlyingLöve's andDewey's analyses of theTriticeae, namely chromosome pairing, is discussed with particular reference to isozyme studies to show that in some cases, such as species ofHordeum sensu lato, consistent relationships are obtained. Finally, new developments in understanding chromosome structure are considered in relation to the above variables in the taxonomy and evolution of theTriticeae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00936706
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