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  • 1
    Electronic Resource
    Electronic Resource
    The Growth in Culture of Detached Ovaries of Wheat (Triticum aestivum L. em. Thell) (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 100 (1988), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Viable embryos have been recovered from fertilized wheat (Triticum aestivum) ovaries detached on the day of, or up to 7 days post-, anthesis and cultured aseptically fur up to 18 days. The most significant factors in determining the yield of embryos was the plating density, age and complexity of the explants. 26% of ovaries excised on the day of anthesis produced viable embryos if cultured as pairs of florets. The potential use of detached ovary cultures in gametophyte microinjection experiments, rescuing embryos from wide crosses and in chemically manipulating the early stages of embryo development are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1988.tb00229.x
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  • 2
    Electronic Resource
    Electronic Resource
    The effect of cefotaxime on the growth and regeneration of callus from four varieties of barley (Hordeum vulgare L.) (1987)
    Springer
    Plant cell reports 6 (1987), S. 454-457 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Recently it has been reported that the cephalosporin antibiotic cefotaxime increases growth, regeneration and embryogenesis in wheat calli. We investigated the effect of cefotaxime on callus initiated from immature embryos of four barley (Hordeum vulgare L.) varieties. In calli cultured in the presence of antibiotic callus growth was up to 45% greater than in controls and the frequency of regenerating calli was increased by up to 80%. There was an apparent interaction of the antibiotic with genotype and the 2,4-D in the medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272781
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  • 3
    Electronic Resource
    Electronic Resource
    Plant regeneration from mesophyll protoplasts of Moricandia arvensis (1992)
    Springer
    Plant cell reports 11 (1992), S. 408-411 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Moricandia arvensis is of interest as it is a dicotyledonous species which has C3 — C4 intermediate photosynthesis, a mechanism which results in enhanced recapture of photorespired CO2. Leaves from cultured shoot tips were used as a source for mesophyll cell protoplasts. Approximately 1% of the protoplasts which survived the first few days of culture produced calli. On a suitable regeneration medium, 30–60% of the calli regenerated one or more shoots. From among the regenerating shoots eight were selected, transferred to soil and grown to flowering in the glasshouse; all were fertile. The development of a protoplast regeneration system provides the opportunity to use transformation and somaclonal variation as tools in the genetic analysis of the C3–C4 character in this species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234371
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  • 4
    Electronic Resource
    Electronic Resource
    Plant regeneration from hypocotyls, cotyledons and stem segments of the C3–C4 intermediate species Moricandia arvensis (1992)
    Springer
    Plant cell reports 11 (1992), S. 412-415 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Techniques have been developed for the regeneration of Moricandia arvensis from complex explants. Hypocotyl segments and cotyledonary explants regenerated shoots, but the most efficient plant regeneration was from stem sections taken from in vitro grown shoots. Regeneration from these three explant types was tested on a range of concentrations of benzylaminopurine and either naphthylene acetic acid or indole acetic acid. Regeneration from all three explants was much higher on indole acetic acid than on naphthylene acetic acid and the ratio of auxin to cytokinin was also significant in determining the response of explants. Optimum regeneration was on 1mg/l IAA with 1mg/l BAP. Plants could be transferred to soil and grown to flowering in the glasshouse.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234372
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  • 5
    Electronic Resource
    Electronic Resource
    The effects of proline, thioproline and methylglyoxal-bis-(guanylhydrazone) on shoot regeneration frequencies from stem explants of B. napus (1996)
    Springer
    Plant cell reports 15 (1996), S. 695-698 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Internode segments from aseptic shoot cultures are the most prolific explants for the regeneration of Brassica shoots in vitro. These explants also have the advantage of not being subject to the genotypic variations in regeneration response observed in hypocotyl and cotyledon explants. Despite reports of 80–100% shoot regeneration from stem explants, observed frequencies are typically 50–60%. Three media additives, proline, thioproline and methylglyoxal-bis-(guanylhydrazone) (MGBG), were tested for their efficacy in promoting shoot regeneration from stem explants of two B. napus varieties, Westar and Cobra. The effects of proline and thioproline on both varieties were neutral or deleterious. In Cobra the MGBG treatments caused a uniform reduction in explant regeneration. However, at low concentrations (0.35μM) MGBG resulted in a 50% increase, to 92%, in regeneration from Westar. The potential of MGBG in promoting explant regeneration in B. napus is discussed in the light of its interaction with the explant genotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231927
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  • 6
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis and plant regeneration from stem explants of Moricandia arvensis (1997)
    Springer
    Plant cell reports 17 (1997), S. 27-31 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsMoricandia arvensis ; Brassicaceae ; C3-C4 intermediate ; Stem explants ; Somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Efficient and reproducible plant regeneration has been established from stem internode explants of Moricandia arvensis, a crucifer of special interest due to its C3-C4 intermediate photosynthetic activity. Somatic embryogenesis was induced in one-third of explants cultured on Murashige and Skoog based medium containing 9 mm 2,4-dichlorophenoxyacetic acid. High frequencies of plant regeneration (〉90%) resulted when somatic embryos were germinated on medium lacking growth regulators. Regenerated plants were diploid, fertile and morphologically similar to seed-derived plants of M. arvensis. This is the first report of somatic embryogenesis in M. arvensis. This plant regeneration system should facilitate gene identification and localisation studies of C3-C4 physiology by insertional mutagenesis, a prerequisite for the isolation and transfer of genes involved in C3-C4 metabolism from Moricandia to cultivated brassicas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050346
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  • 7
    Electronic Resource
    Electronic Resource
    The effect of specific chromosome and cytoplasm substitutions on the tissue culture response of wheat (Triticum aestivum) callus (1986)
    Springer
    Theoretical and applied genetics 71 (1986), S. 797-800 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Callus ; Regeneration ; hromosomes ; Cytoplasm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Calli were initiated from immature embryos of four lines of hexaploid wheat (Triticum aestivum L. em. Thell), the euplasmic nuclear donor ‘Chinese Spring’, ‘Chinese Spring’ in which both 4B chromosomes were substituted by those of the variety ‘Cappelle-Desprez’ and two alloplasmic lines in which these nuclei were substituted into the cytoplasm of Aegilops ovata. The calli were found to differ in their initia growth rates and their ability to organise shoot primordia and regenerate shoots. The ‘Cappelle’ 4B chromosomes had a very significant effect on all these characters. The potential for modelling genotypes for improved tissue culture characteristics is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276420
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  • 8
    Electronic Resource
    Electronic Resource
    Cytoplasmic effects on the tissue culture response of wheat (Triticum aestivum) callus (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 70-75 
    Details
    ISSN: 1432-2242
    Keywords: Cytoplasm ; Wheat ; Tissue culture ; Callus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Calli were initiated from immature embryos of eight lines of hexaploid wheat (Triticum aestivum L. em. Thell) with different cytoplasms, the euplasmic nuclear donor ‘Chinese Spring’ and seven alloplasmic lines derived from wild relative species of the genera Triticum and Aegilops. The calli were found to differ in their initial growth rates, their sensitivity to 2,4-D and their ability to organise shoot primordia, demonstrating that the cytoplasm can significantly affect the behaviour of tissues in culture. The potential for improving the responses of tissues in culture by cytoplasmic changes is noted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261457
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  • 9
    Electronic Resource
    Electronic Resource
    In vitro expression of genes affecting whole plant phenotype — the effect of Rht/Gai alleles on the callus culture response of wheat (Triticum aestivum L. em. Thell) (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 474-479 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Callus ; Regeneration ; Dwarfinggenes ; Rht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Calli were initiated from immature embryos of 12 lines of hexaploid wheat (Triticum aestivum L. em. Thell). The lines were from 3 varieties — April Bearded, Bersee and Maris Huntsman — isogenic for the reduced height/gibberellic acid insensitivity (Rht) genes — Rht1, Rht2 and Rht3 — and the ‘tall’ (rht) allele. The dwarfing genes had significant effects on the growth and morphogenesis of calli. The genes interacted with the 2,4-D in the medium and the varietal background. Calli of each line were cultured in the presence and absence of 1 mg/l of gibberellic acid (GA), but there was no interaction of the Rht genes with GA in vitro. The effect of the Rht genes is discussed in relation to their effects on cellular hormone metabolism and their involvement in previously described chromosome 4B effects in culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276752
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  • 10
    Electronic Resource
    Electronic Resource
    Expression of the intermediate character in somatic hybrids between Brassica napus and the species Moricandia arvensis (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 1234-1241 
    Details
    ISSN: 1432-2242
    Keywords: Key wordsBrassica napus  ;  Moricandia arvensis  ;  Somatic hybridisation  ;   intermediate  ;   Photorespiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The wild crucifer Moricandia arvensis is a potential source of alien genes for the genetic improvement of related Brassica crops. In particular M. arvensis has a intermediate photosynthetic mechanism which results in enhanced recapture of photorespired CO2 and may increase plant water-use efficiency. In order to transfer this trait into Brassica napus, somatic hybridisations were made between leaf mesophyll protoplasts from cultured M. arvensis shoot tips and hypocotyl protoplasts from three Brassica napus cultivars, ‘Ariana’, ‘Cobra’ and ‘Westar’. A total of 23 plants were recovered from fusion experiments and established in the greenhouse. A wide range of chromosome numbers were observed among the regenerated plants, including some apparent mixoploids. Thirteen of the regenerated plants were identified as nuclear hybrids between B. napus and M. arvensis on the basis of isozyme analysis. The phenotypes of these hybrids were typically rather B. napus-like, but much variability was observed, including variation in flower colour, leaf shape and colour, leaf waxiness, fertility and plant vigour. CO2 compensation point measurements on the regenerated plants demonstrated that 3 of the hybrids express the M. arvensis intermediate character at the physiological level. Semi-thin sections through leaf tissues of these 3 plants revealed the presence of a Kranz-like leaf anatomy characteristic of M. arvensis but not found in B. napus. This is the first report of the expression of this potentially important agronomic trait, transferred from Moricandia, in M. arvensis×B. napus hybrids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050361
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