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  • 1
    Electronic Resource
    Electronic Resource
    Subcellular localization of glutelin-2 in maize (Zea mays L.) endosperm (1984)
    Springer
    Plant molecular biology 3 (1984), S. 227-234 
    Details
    ISSN: 1573-5028
    Keywords: endosperm ; immunoelectron microscopy ; glutelins ; maize ; protein blotting ; protein bodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Accumulation of the 28 KD protein of the glutelin-(G2) fraction was followed in developing maize endosperm, using sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) and peak integration of scanned gels. 28 KD glutelin-2 could already be observed from 15 days after pollination and its accumulates reached a plateau during the second half of the development period. The process of biosynthesis of 28 KD glutelin-2 and zeins occurs in a parallel way. Subcellular fractions obtained from linear sucrose gradient centrifugation of developing maize endosperms were analyzed by SDS-PAGE and immunoblotting using a serum reacting against glutelin-2 and 14 KD Z2. Glutelin-2 was found to be present in the protein bodies when subcellular fractionation was carried out without dithiothreitol (DTT). The presence of a reducing agent causes the elution of glutelin-2 from protein bodies. Immunocytochemical labelling using the protein A-colloidal gold technique in protein bodies incubated with anti-G2 IgG revealed that G2 is located mainly in the periphery of protein bodies. These results are interpreted as indicating a structural role for glutelins in protein bodies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029658
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  • 2
    Electronic Resource
    Electronic Resource
    Chromosome localization and characterization of a family of long interspersed repetitive DNA elements from the genus Zea (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 1094-1100 
    Details
    ISSN: 1432-2242
    Keywords: Genus Zea ; Repeated DNA sequences ; In situ hybridization ; Zea diploperennis ; Maize chromosome localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper describes the characterization and chromosomal distribution of new long repetitive sequences present in all species of the genus Zea. These sequences constitute a family of moderately repetitive elements ranging approximately from 1350 to 1700 copies per haploid genome in modern maize (Zea mays ssp. mays) and teosinte (Zea diploperennis), respectively. The elements are long, probably larger than 9 kb, and they show a highly conserved internal organization among Zea subspecies and species. The elements are present in all maize chromosomes in an interspersed pattern of distribution, are absent from centromeric and pericentric heterochromatin, and with some clustering in the distal regions of chromosome arms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222927
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