ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

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Electronic Resource

Analysis of stable events of transformation in wheat via PEG-mediated DNA uptake into protoplasts

Ajmone Marsan, P. ; Lupotto, E. ; Locatelli, F. ; [et al.]

Amsterdam : Elsevier

Plant Science 93 (1993), S. 85-94

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ISSN: 0168-9452

Keywords: Kanamycin ; Molecular analysis ; Transformation ; Triticum aestivum

Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Topics: Biology

Type of Medium: Electronic Resource

URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(93)90037-Z

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2

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Transformation of Solanum integrifolium poir via Agrobacterium tumefaciens: Plant regeneration and progeny analysis (1992)

Rotino, G. L. ; Perrone, D. ; Ajmone-Marsan, P. ; [et al.]

Springer

Plant cell reports 11 (1992), S. 11-15

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ISSN: 1432-203X

Keywords: Agrobacterium tumefaciens ; Solanum integrifolium ; transformation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary The wild species Solanum integrifolium represents a source of pest and disease resistance genes for breeding strategies of the cultivated species Solanum melongena. Somatic hybridization via protoplast fusion between the two species may provide a valuable tool for transferring polygenic traits into the cultivated species. The availability of S.integrifolium cells carrying dominant selectable markers would facilitate the heterokaryon rescue. An appropriate methodology for in vitro culture and plant regeneration from leaf explants of S.integrifolium is reported. Efficient leaf-disk transformation via co-cultivation with Agrobacterium tumefaciens led to the regeneration of transformed plants carrying the reporter genes GUS and NPT-II. Transformed individuals were obtained through selection on kanamycin-containing medium. Stable genetic transformation was assessed by histochemical and enzymatic assays for GUS and NPT-II activity, by the ability of leaf disks to initiate callus on Km-containing medium, Southern blot analyses of the regenerated plants, and genetic analysis of their progenies. Selfed-seed progeny of individual transformed plants segregated seedlings capable to root and grow in selective condition, while untransformed progeny did not. Genetic analyses of progeny behaviour showed that the reporter gene NPT-II segregated as single as well as two independent Mendelian factors. In two cases an excess of kanamycin-sensitive seedlings was obtained, not fitting into any genetic hypothesis.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00231831

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3

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Genetic diversity and its relationship to hybrid performance in maize as revealed by RFLP and AFLP markers (1998)

Ajmone Marsan, P. ; Castiglioni, P. ; Fusari, F. ; [et al.]

Springer

Theoretical and applied genetics 96 (1998), S. 219-227

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ISSN: 1432-2242

Keywords: Key words DNA polymorphisms ; Genetic distances ; Molecular markers ; Yield prediction ; Zea mays

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The challenge to maize breeders is to identify inbred lines that produce highly heterotic hybrids. In the present study we surveyed genetic divergence among 13 inbred lines of maize using DNA markers and assessed the relationship between genetic distance and hybrid performance in a diallel set of crosses between them. The parental lines were assayed for DNA polymorphism using 135 restriction fragment length polymorphisms (RFLPs) and 209 amplified-fragment polymorphisms (AFLPs). Considerable variation among inbreds was detected with RFLP and AFLP markers. Moreover AFLPs detect polymorphisms more efficiently in comparison to RFLPs, due to the larger number of loci assayed in a single PCR reaction. Genetic distances (GDs), calculated from RFLP and AFLP data, were greater among lines belonging to different heterotic groups compared to those calculated from lines of the same heterotic group. Cluster analysis based on GDs revealed associations among lines which agree with expectations based on pedigree information. The GD values of the 78 F1 crosses were partioned into general (GGD) and specific (SGD) components. Correlations of GD with F1 performance for grain yield were positive but too small to be of predictive value. The correlations of SGDs, particularly those based on AFLP data, with specific combining-ability effects for yield may have a practical utility in predicting hybrid performance.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001220050730

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4

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AFLP markers in a molecular linkage map of maize: codominant scoring and linkage group ditsribution (1999)

Castiglioni, P. ; Ajmone-Marsan, P. ; van Wijk, R. ; [et al.]

Springer

Theoretical and applied genetics 99 (1999), S. 425-431

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ISSN: 1432-2242

Keywords: Key words Genetic map ; Linkage analysis ; AFLP ; Methylation sensitivity ; Codominant markers ; Zea mays L.

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract We exploited the AFLP technique to saturate a RFLP linkage map derived from a maize mapping population. By using two restriction enzyme, EcoRI and PstI, differing in methylation sensitivity, both in combination with MseI, we detected 1568 bands of which 340 where polymorphic. These were added to the exitsing RFLP marker data to study the effects of incorporation of AFLPs produced by different restriction-enzyme combinations upon genetic maps. Addition of the AFLP data resulted in greater genome coverage, both through linking previously separate groups and the extension of other groups. The increase of the total map length was mainly caused by the addition of markers to telomeric regions, where RFLP markers were poorly represented. The percentage of informative loci was significantly different between the EcoRI and PstI assays. There was also evidence that PstI AFLP markers were more randomly distributed across chromosomes and chromosome regions, while EcoRI AFLP markers clustered mainly at centomeric regions. The more-random ditsribution of PstI AFLP markers on the genetic map reported here may reflect a preferential localisation of the markers in the hypomethylated telomeric regions of the chromosomes.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001220051253

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5

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Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs, and AFLPs (1998)

Pejic, I. ; Ajmone-Marsan, P. ; Morgante, M. ; [et al.]

Springer

Theoretical and applied genetics 97 (1998), S. 1248-1255

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ISSN: 1432-2242

Keywords: Key words Zea mays L. ; Genetic relationship ; Molecular markers ; DNA-fingerprinting ; Genetic diversity

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract DNA-based fingerprinting technologies have proven useful in genetic similarity studies. RFLP is still most commonly used in the estimation of genetic diversity in plant species, but the recently developed PCR-based marker techniques, RAPDs, SSRs and AFLPs, are playing an increasingly important role in these investigations. Using a set of 33 maize inbred lines we report on a comparison of techniques to evaluate their informativeness and applicability for the study of genetic diversity. The four assays differed in the amount of polymorphism detected. The information content, measured by the expected heterozygosity and the average number of alleles, was higher for SSRs, while the lowest level of polymorphism was obtained with AFLPs. However, AFLPs were the most efficient marker system because of their capacity to reveal several bands in a single amplification. In fact, the assay efficiency index was more than ten-fold higher for AFLPs compared to the other methods. Except for RAPDs, the genetic similarity trees were highly correlated. SSR and AFLP technologies can replace RFLP marker in genetic similarity studies because of their comparable accuracy in genotyping inbred lines selected by pedigree. Bootstrap analysis revealed that, in the set of lines analysed, the number of markers used was sufficient for a reliable estimation of genetic similarity and for a meaningful comparison of marker technologies.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001220051017

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6

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In an elite cross of maize a major quantitative trait locus controls one-fourth of the genetic variation for grain yield (1995)

Ajnone-Marsan, P. ; Monfredini, G. ; Ludwig, W. F. ; [et al.]

Springer

Theoretical and applied genetics 90 (1995), S. 415-424

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ISSN: 1432-2242

Keywords: RFLP markers ; Heterosis ; QTL mapping ; Testcross performance ; Zea mays L.

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Quantitative trait loci (QTLs) for grain yield, dry matter content and test weight were identified in an F2 segregating population derived from a single cross between two elite maize lines (B73 and A7) and testcrossed to two genetically divergent in breds. Most of the QTLs inferred were consistent across locations, indicating that the expression of the genes influencing the traits under investigation was largely independent of the environment. By using two different tester lines we found that QTLs exhibited by one tester may not necessarily be detected with the second one. Only loci with larger effects were consistent across testers, suggesting that interaction with tester alleles may contribute to the identification of QTLs in a specific fashion. Analysis across both testers revealed four significant QTLs for grain yield that explained more than 35% of the phenotypic variation and showed an overall phenotypic effect of more than 2t/ha. The major QTL for grain yield, located in the proximity of the Nucleolus Organiser Region, accounted for 24.5% of the phenotypic variation for grain yield and showed an average effect of allele substitution of approximately 1 t/ha. Marker-assisted introgression of the superior A7 allele at this locus in the B73 genetic background will not differ from qualitative trait introgression and will eventually lead to new lines having superior testcross performance.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00221984

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7

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Genetic diversity of maize inbred lines within and among heterotic groups revealed by RFLPs (1992)

Livini, C. ; Ajmone-Marsan, P. ; Melchinger, A. E. ; [et al.]

Springer

Theoretical and applied genetics 84 (1992), S. 17-25

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ISSN: 1432-2242

Keywords: Maize ; RFLPs ; Genetic diversity ; Heterotic groups

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary The objectives of this study were (1) to investigate genetic diversity for RFLPs in a set of important maize inbreds commonly used in Italian breeding programs, (2) to compare genetic similarities between unrelated lines from the same and different heterotic groups, and (3) to examine the potential of RFLPs for assigning maize inbreds to heterotic groups. Forty inbreds were analyzed for RFLPs with two restriction enzymes (EcoRI and HindIII) and 82 DNA clones uniformly distributed over the maize genome. Seventy clone-enzyme combinations gave single-banded RFLP patterns, and 79 gave multiple-banded RFLP patterns. The average number of RFLP patterns detected per clone-enzyme combination across all inbreds was 5.8. RFLP data revealed a wide range of genetic diversity within the two heterotic groups assayed, Iowa Stiff Stalk Synthetic (BSSS) and Lancaster Sure Crop (LSC). Genetic similarity (GS) between lines was estimated from binary RFLP data according to the method of Nei and Li (1979). The mean GS for line combinations of type BSSS × LSC (0.498) was substantially smaller than for unrelated line combinations or type BSSS × BSSS (0.584) but almost as great as for un-related line combinations of type LSC × LSC (0.506). Principal coordinate and cluster analyses based on GS values resulted in the separate groupings of lines, which is consistent with known pedigree information. A comparison between both methods for multivariate analyses of RFLP data is presented.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00223976

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8

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A detailed analysis of two seismic sequences in Abruzzo, Central Apennines, Italy (2000)

De Luca, G. ; Scarpa, R. ; Filippi, L. ; [et al.]

Springer

Journal of seismology 4 (2000), S. 1-21

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ISSN: 1573-157X

Keywords: Apennines ; focal mechanisms ; seismicity ; source parameters

Source: Springer Online Journal Archives 1860-2000

Topics: Geosciences , Physics

Notes: Abstract A three-component digital seismic network has been installed along central Apennines since the end of 1991. Two seismic sequences having main shocks of magnitudes 3.9 and 3.7 were recorded in August 1992 and June 1994, respectively. A detailed analysis of these sequences, including multiplet relocation, fault-plane solutions and source parameter estimation, is performed in the present paper. A correlation analysis allowed us to recognize a number of correlated events in the two sequences which were used for relative locations using a master event technique. This analysis allowed to obtain a better alignment of epicentral data along two almost orthogonal directions, following an Apenninic and an anti-Apenninic trend. For the two sequences, fault-plane solutions were evaluated by using a first arrival technique, resulting in mechanisms with predominant normal faulting for the 1992 and 1994 swarms. S-wave polarization analysis allowed to check the stability of the previous solutions and to reduce their range of uncertainty. The same technique was also applied to derive the composite fault-plane solutions from the aftershocks, resulting in solutions which are in good agreement with those derived from the main shocks of both sequences. Source parameters were then derived from the three-component records of 28 well-recorded events with seismic moment in the range 8.5 × 1010–1.0 × 1014 Nm. Stress drops ranged in the interval 0.3–52.3 bar and source radii were of the order of 100 m. Their scaling relations are in good agreement with other results derived from the analysis of other Italian earthquakes that occurred in regions of predominantly normal faulting tectonics (Apennines and Calabrian arc).

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1009870327083

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Cluster analysis of RFLP data from related maize inbred lines of the BSSS and LSC heterotic groups and comparison with pedigree data (1992)

Ajmone-Marsan, P. ; Livini, C. ; Messmer, M. M. ; [et al.]

Springer

Euphytica 60 (1992), S. 139-148

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ISSN: 1573-5060

Keywords: coancestry coefficient ; genetic similarity ; maize ; Zea mays

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Summary In this study, 31 elite inbred lines of maize (Zea mays L.) were analyzed with 149 clone-enzyme combinations for their respective RFLP profiles. Objectives were (1) to determine the utility of RFLPs for estimation of genetic similarties among 16 inbred lines from the Iowa Stiff Stalk Synthetic (BSSS) and among 15 inbred lines from the Lancaster Sure Crop (LSC) heterotic groups and (2) to compare genetic similarities based on molecular markers with those based on pedigree information. Coefficients of genetic similarity (GS) and coancestry (f) between pairs of lines from the same heterotic group were calculated from RFLP and pedigree data, respectively. For lines from the BSSS heterotic group, cluster analyses based on RFLP and pedigree data revealed similar associations. GS and f values were closely correlated (r=0.70) for related BSSS lines. For lines from the LSC heterotic group, considerable discrepancies existed between the GS and f values, especially for those pairs involving inbreds Va22 and Lo924. Effect of selection and/or erroneous pedigree records are discussed as possible explanations for the low correlation of GS and f values (r=0.07) for related LSC lines. RFLPs seem useful for investigation of relationships among maize inbreds, verification of pedigree records, and quantification of the degree of relatedness.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00029669

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