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  • 1
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: After 10 years of evaluation in different locations with high levels of incidence of disease, a group of sugarcane somaclones derived from callus tissues was selected for eyespot resistance. Resistance evaluations of four somaclones were performed under field and laboratory conditions. The results confirmed the superiority of two somaclones, one resistant and one tolerant to eyespot disease. Restriction analysis of mitochondrial DN A revealed that the two somaclones had a different DNA organization which distinguished them both from each other and from the donor plant; the restriction profile was similar however to that of the resistant control done. Restriction patterns of a third somaclone, also resistant, were similar to those of the donor plant. Differences among the somaclones were also evident when using a maize ribosomal DNA probe.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 107 (1991), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Sugarcane leaves and calli from highly susceptible and resistant varieties to eyespot disease were used to evaluate the Drechslera sacchari toxin effect at different concentrations and incubation times by measuring electrolyte leakage. This expression of disease resistance was observed not only in the leaf but also in the callus. Furthermore, the growth of resistant calli MS medium supplemented with DS toxin, was higher when compared to the susceptible ones. The possibility of obtaining resistant somaclones is confirmed.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 102 (1989), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Two methods are proposed to evaluate rust and eye spot disease resistance in sugarcane using biochemical criteria.Eye spot disease resistance is easily evaluated by a fast conductimetric bioassay of high sensitivity. If desired, the conductimetric values may be converted into grades of field resistance by means of a regression graph based on standard varieties. The main advantages of this method art it's complete objectivity, reproducibility and independence of climatic conditions.It was also found that susceptibility of sugarcane to rust can be recognized very early in the infection process by a striking increment of peroxidase activity. The simplicity of this enzyme reaction, which can easily be automated and the speed of detection during infection are discussed as an option for rust resistance evaluation.
    Type of Medium: Electronic Resource
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