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  • 1
    ISSN: 1520-4804
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Unikaryon matteii n. sp. (Microsporida, Unikaryonidae) parasite le tube digestif, les tubes de Malpighi, les muscles et le tissu adipeux de Nisotra sp. (Coleoptera, Chrysomelidae). Elle se développe en contact direct avec le cytoplasme de la cellule hôte et ne présente jamais de diplocaryon.Les mérontes sont limités par une simple membrane plasmique. Leur cytoplasme présente quelques saccules de reticulum endoplasmique et des vésicules golgiennes. Les mérontes uninucléés sont globuleux (2 μm de diamèGtre). Les mérontes binucléés sont allongés (5 × 1.5 μm) et se divisent par simple étranglement médian pour donner deux nouveaux mérontes uninucléés, qui pourront évoluer en sporontes.La sporogonie est disporoblastique. Les sporontes et les sporoblastes sont limités par une paroi d'environ 15 nm d'épaisseur, et leur cytoplasme présente un reticulum endoplasmique développé. Les sporontes uninucléés (2 μm de diamétre) deviennent binucéés (5 × 2 μm), puis subissent une division binaire pour donner deux sporoblastes uninucléés. Les sporoblastes, d'abord de forme irrégulièGre deviennent ovoïdes (2.5 × 2 μm) puis évoluent en spores.Les spores sont ovales et uninucléées (3.72 ± 0.39 μm × 1.96 ± 0.15 μm). Elles possèGdent un filament polaire qui décrit 5 à 12 tours de spires. Leur polaroplaste est uniquement lamellaire, mais subdivisé en deux parties: I'une, antérieure, constituée de saccules aplatis, I'autre, postérieure, constituée de saccules dilatés.La description d'une deuxièGme espèGce d'Unikaryon chez les coléoptères confirme le développement de ces parasites chez les insectes, et pose le problèGme de la validité de certaines Nosema d'insectes dont les spores sont uninucléées.〈section xml:id="abs1-2"〉〈title type="main"〉ABSTRACT Unikaryon matteii n. sp. (Microsporida, Unikaryonidae) is a parasite of gut, Malpighian tubules, muscles, and fat body of Nisotra sp. (Coleoptera, Chrysomelidae). It develops diffusely in the host cell cytoplasm and is unikaryotic throughout merogony and sporogony.The meronts are surrounded by a simple plasma membrane. Their cytoplasm contains little endoplasmic reticulum and some Golgi vesicles. Uninucleate meronts are globular (2 μm in diameter). Binucleate meronts are elongate (5 × 1.5 μm); by binary fission they give rise to two uninucleate meronts, which may develop into sporonts.Sporogony is disporoblastic. Sporonts and sporoblasts are bounded by an electron-dense wall about 15 nm thick, and their cytoplasm contains some flattened cisternae of endoplasmic reticulum. The uninucleate sporonts (2 μm in diameter) become binucleate (5 × 1.5 μm) and undergo binary fission to produce two uninucleate sporoblasts. The sporoblasts, at first irregular in outline, become ovoid (2.5 × 2 μm) and give rise to spores.The spores are uninucleate and oval (3.72 ± 0.39 μm × 1.96 ± 0.15 μm). They have a polar filament with 5 to 12 coils and a Iamellate polaroplast divided into two parts: an anterior part composed of flattened sacs and a posterior part made up of swollen sacs.The description of a second species of Unikaryon in a coleopteran confirms the development of these parasites in insects. The validity of some Nosema of insects which have uninucleate spores is discussed.
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  • 3
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The mature Paranoplocephala omphalodes spermatozoon is filiform, tapered at both ends and lacks mitochondria. Its anterior extremity exhibits an apical cone of electron-dense material measuring about 900 nm in length and 200 nm in width, and two crested-like bodies. The cortical microtubules follow a 25–35° helicoidal path along their whole length, except at the posterior extremity where they become parallel to the spermatozoon axis. They are arranged in a single or two fields which may cover each other partially. The axoneme, of the 9+“1” pattern of the Trepaxonemata, lacks a peri-axonemal sheath and does not reach the extremity of the spermatozoon. The nucleus is a compact and irregular cord coiled in a spiral around the axoneme. Moreover, we report for the first time a nucleus in the spermatozoon of a Cyclophyllidea species which reaches beyond the axonemal posterior extremity. The cytoplasm, depending on the level where the section is cut, is slightly electron dense or electron lucent and contains numerous small electron-dense granules in regions III–V. In the posterior spermatozoon extremity, granular material is replaced by a terminal and compact electron-dense material.
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  • 4
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Spermiogenesis and mature spermatozoa of Opecoeloides furcatus (Digenea, Opecoelidae) are described by means of transmission electron microscopy. Spermiogenesis in this fluke matches the general pattern of digenetic trematodes. Striated rootlets associated with the two centrioles and an intercentriolar body are present in the differentiation zone. Flagellar rotation of two flagella and their proximodistal fusion with a median cytoplasmic process also characterize the spermiogenesis of O. furcatus. Nevertheless, asynchronicity is reported for the proximodistal fusion of the two flagella. Mature spermatozoa of O. furcatus are filiform, tapering at both ends and they present all the characteristic features found in the Digenea gamete: two flagella, mitochondrion, nucleus and two bundles of parallel cortical microtubules. Nevertheless several peculiarities distinguish the mature spermatozoon of O. furcatus from the gamete of other digenetic trematodes.
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  • 5
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We report for the first time the ultrastructure of the spermatozoon of a Dilepididae cestode. The mature spermatozoa of Dipylidium caninum is filiform and tapered at both ends and lacks mitochondria. The anterior extremity exhibits an apical cone of electron-dense material measuring about 600 nm in length and a single helicoidal crested-like body that is 150 nm thick. This single crested-like body is spiralized at an angle of about 40° to the spermatozoon axis. The axoneme, showing the 9 + “1” pattern of the Platyhelminthes, lacks a periaxonemal sheath. The cortical microtubules constitute a continuous, submembranous, electron-dense material, and they are also twisted at an angle of about 40° to the spermatozoon axis. Moreover, the nucleus is coiled in a helix around the axoneme, which is centrally located throughout its length. We demonstrate for the first time the presence of peripheral microtubules twisted to the spermatozoon axis up to the posterior extremity of the gamete.
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  • 6
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Nosema birgii n.sp. (Microsporida, Nosematidae) a été trouvée dans les larves et adultes deMesoplatys cincta (Coleoptera, Chrysomelidae) du Sénégal. Elle attaque divers tissus et provoque une infection généralisée. Son cycle de développement et l'ultrastructure de ses stades sont typiques du genreNosema. Les spores vivantes sont ovales et allongées et mesurent 6,20±0,21 μm×3,5±0,18 μm. Leur filament polaire décrit 12 à 14 tours de spire et leur polaroplaste possède une partie antérieure lamellaire et une partie postérieure vésiculeuse.
    Notes: Abstract Nosema birgii n.sp. (Microsporida, Nosematidae) was found in larvae and adults ofMesoplatys cincta (Coleoptera, Chrysomelidae) from Senegal. It attacked various tissues and caused a general infection. Its life cycle and the ultrastructure of its stages were typical of the genusNosema. The living spores were oval, elongate and measured 6.20±0.21 μm×3.5±0.18 μm. Their polar filament formed 12 to 14 coils and their polaroplast was lamellar and vesicular.
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  • 7
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: To validate a recently developed liquid chromatography/ultraviolet (LC/UV) method in a pharmacokinetic study of Vectarion® (almitrine) where the unchanged drug, its five metabolites and an internal standard are analysed, on-line fast atom bombardment liquid chromatography/mass spectrometry (FAB LC/MS) with a moving belt interface has been used. Since the liquid chromatographic conditions required the use of perchloric acid FAB was chosen as ionization mode for the involatile perchlorates formed in the liquid chromatographic process. The use of FAB LC/MS allows the detection of the protonated molecular ions of all the components in the mixture. Furthermore, all the compounds show an intense peak at m/z 203, presenting a liquid chromatographic profile which is very similar to the profile obtained with UV detection.
    Additional Material: 3 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 33 (1992), S. 39-45 
    ISSN: 1040-452X
    Keywords: Platyhelminthes ; Hymenolepidiadae ; Crested-like bodies ; Cortical microtubules ; Spermatid ; Orientation of spermatozoon ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Spermiogenesis in Hymenolepsis nana begins with the formation of a differentiation zone. This is limited at the front by arched membranes, is surrounded by cortical microtubules assoicated with 12 crested-like bodies, and contains a single centriole made up of doublets. The distal centriole gives rise to a flagellum that grows at the same pace as the cortical microtubules. Migration of the nucleus takes place after the formation of the flagellum. It is followed by the separation of the old spermatid from the residual cytoplasm. The mature H. nana spermatozoon is filiform and lacks mitochondria. The axoneme, of the 9 + “1” pattern of the Platyhelminthes, does not reach the extremities of the spermatozoon. The nucleus is electron dense and is in close contact with the axoneme around which it coils in a spiral making an angle of 10° to 15° with the spermatozoon axis. The cortical microtubules follow a 10° to 15° helicoidal path along almost their whole length, except at their posterior extremity, where they are parallel to the spermatozoon axis. H. nana is distinguished by the early development of 12 crested-like bodies of different lengths and by the existence of a single centriole in the differentiation zone. Such a high number of crested-like bodies had never previously been reported in a cestode. © 1992 Wiley-Liss, Inc.
    Additional Material: 14 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 91 (1958), S. 401-404 
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Die Isolierung der freien Dihydroxy-oxyd-laevopimarsäure aus dem Gemisch der Oxydationsprodukte der Laevopimarsäure mit Permanganat wird beschrieben, ferner werden die chemischen Eigenschaften dieser Verbindung angegeben.
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  • 10
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 91 (1958), S. 405-407 
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Bei der Oxydation von Laevopimarsäure-methylester mit Kaliumpermanganat in nichtwäßrigen Lösungsmitteln wurde als Reaktionsprodukt Dehydroabietin-säure-methylester erhalten.
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