ISSN:
1432-0983
Keywords:
DNA preparation by osmotic shock
;
Plant mitochondrial genomes
;
Multiple size classes of mitochondrial genomes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary DNA was isolated from DNase-treated, osmotically shocked mitochondria from leaves and calli of Nicotiana plumbaginifolia. Electron microscopic analysis only revealed linear molecules, although the same technique yielded a sizable fraction of circular mitochondrial DNA molecules in the fission yeast Schizosaccharomyces pombe. Most of the plant mitochondrial DNA molecules could be assigned to six size classes of 3.11 ± 0.19, 7.08 ± 0.53, 11.2 ± 0.29, 14.10 ± 0.23, 18.13 ± 0.39, and 23.17 ± 0.51 μm length (mean ± 95% confidence intervals). These results are compared with those obtained for mitochondrial genomes of other higher plants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00421613
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