Publication Date:
2016-09-09
Description:
The objective of this study was to evaluate complex biological properties of human stem cells isolated from adipose tissue (ASCs) harvested utilizing different methods: surgical resection (R), power assisted liposuction (PAL) and laser assisted liposuction (LAL). ASCs were isolated from healthy donors, due to surgical resection, power and laser assisted liposuction. Isolated cells were characterized by their clonogenicity, proliferation rate, doubling time, multilineage differentiation and senescence potential. The average number of ASCs from 1g/1ml of solid adipose tissue/lipoaspirate was 2.9 × 10 5 ± 2.4 × 10 5 , 1.1 × 10 5 ± 0.8 × 10 5 and 1.2 × 10 5 ± 0.7 × 10 5 respectively for ASCsR, ASCsPAL and ASCsLAL. However, number of colonies formed by ASCsR and ASCsPAL was significantly higher compared to the average number of colonies formed by ASCsLAL Also, in comparison to other analyzed cell groups, ASCsPAL obtained the highest proliferative activity. All analyzed cells were characterized by stable expression of CD90 and CD44 markers during prolonged culture. Expression of CD34 and CD45 markers was decreasing in subsequent passages. Presented study shows that different ASCs collection method affects some basic characteristics of these cells, such as number of isolated cells, clonogeneity or doubling time. This article is protected by copyright. All rights reserved
Electronic ISSN:
0091-7419
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
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