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  • 1
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Isolates of Fusarium oxysporum f.sp. gladioli were collected from widely different geographic areas. These isolates were characterized by pathogenicity to two differential gladiolus cultivars, vegetative compatibility, and total genomic DNA restriction fragment length polymorphisms (RFLPs). RFLPs were used to estimate the genetic divergence and relationship among isolates of F. oxysporum. RFLPs were detected by Southern blot hybridization of total genomic DNA with a 3-4 kb DNA probe generated from total DNA off. oxysporum f.sp. dianthi. Cluster analysis allowed the division of pathogenic strains into three main RFLP groups, each group containing strains with similarity coefficients ranging from 78 to 100%. RFLP groups correlated with vegetative compatibility groups, not with races. Two single pathogenic isolates which could not be assigned to any of the three main vegetative compatibility groups also had distinctive RFLP patterns. Little genetic polymorphism was observed within vegetative compatibility groups, whereas the majority of RFLPs occurred between vegetative compatibility groups, suggesting a common ancestry for strains within a specific vegetative compatibility group and a polyphyletic origin for the present special form gladioli.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 263 (2000), S. 271-280 
    ISSN: 1617-4623
    Keywords: Key wordsFusarium spp. ; Short interspersed nuclear elements (SINEs) ; AFLP fingerprint ; Repetitive DNA ; Gamma irradiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel family of short interspersed nuclear elements (SINEs) has been identified in Fusarium oxysporum. This family has been called Foxy. The feature that makes Foxy unique among SINEs is the presence of 5′ terminal tetranucleotide repeats. Both the number and the sequence of these repeats vary between individual members of the family. The genome of F. oxysporum f. sp. lycopersici contains at least 160 copies of Foxy. In a mutant obtained upon gamma irradiation of a wild-type isolate, 13 new Foxy insertions were identified. These observations, together with the occurrence of many Foxy-specific polymorphisms between isolates within one vegetative incompatibility group and the presence of Foxy-specific transcripts in the fungus, indicate that Foxy is currently active and may contribute to the genetic variability of F. oxysporum. Since we have not been able to detect Foxy sequences by PCR analyses in other fungi, this novel family of SINEs seems to be confined to Fusarium species.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    European journal of plant pathology 98 (1992), S. 57-64 
    ISSN: 1573-8469
    Keywords: Gladiolus ; Iridaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The pathogenicity and vegetative compatibility of mainly Dutch isolates ofFusarium oxysporum collected from diseased gladioli and other Iridaceae were investigated. Based on their pathogenicity to two differential gladiolus cultivars, the isolates could tentatively be divided into two races. All self-compatible isolates ofFusarium oxysporum f.sp.gladioli belonged to one of three distinct vegetative compatibility groups, VCG 0340, 0341 or 0342, and were incompatible with isolates that were not pathogenic to gladiolus. Isolates of one of the two races were restricted to one VCG while isolates of the other race were present in all three VCGs.
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