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  • 1
    Electronic Resource
    Electronic Resource
    Localization of Phosphatases in Trypanosoma rhodesiense (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Phosphatase activity in Trypanosoma rhodesiense has been examined histochemically by light and electron microscopy and by enzymatic assay in homogenate fractions. Using a method with lead as capture ion, acid phosphatase was found in lysosome-like vesicles and in the flagellar pocket. No alkaline adenosine triphosphatase (ATPase) was detectable by this method. Direct assay of p-nitrophenylphosphatase activity in homogenate fractions showed that acid phosphatase activity was strongly membrane-bound, but that activity at pH 9 was minimal in both soluble and particulate fractions. “Endogenous” ATPase activity was localized specifically and reproducibly in the mitochondrial membranes and under the plasma membrane of the flagellum. This nonenzymic reaction product could not be eradicated by glycerol extraction or glucose depletion. Unlike the membrane staining, which was manifest only after lead treatment, heat-resistant electron-dense material was found in the matrix of lysosomal vesicles in trypanosomes fixed in glutaraldehyde only and not subjected to further treatment with heavy metal reagents. X-ray emission analysis showed the presence of calcium and phosphorus, indicating that the matrix might have a phosphate storage function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1981.tb05320.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Blood flukes have a double outer membrane (1977)
    [s.l.] : Nature Publishing Group
    Nature 269 (1977), S. 147-149 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 Electron micrographs showing the double outer membrane of representative specimens from each of the three families of blood flukes, a, Schistosoma japonicum (Schistosomatidae); 6, Schistosoma margrebowiei (Schistosomatidae); c, Apowcotyle simplex (Aporocotylidae); d, Spirorchis sp. ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/269147a0
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  • 3
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    Blood flukes have a double outer membrane (1977)
    Springer Nature
    Details
    Publication Date: 1977-09-01
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Published by Springer Nature
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