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  • 1
    Electronic Resource
    Electronic Resource
    CROSS-LINKING OF Ly 6-LINKED ALLOANTIGENS: ASSOCIATION BETWEEN ThB AND Ly 5 (1989)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 16 (1989), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The bifunctional cross-linking reagent dithiobis (succinimidyl propionate) (DSP) was used to cross-link 125I surface-labelled glycoproteins from viable thymocytes. The cells were solubilized, and the cross-linked material immunoprecipitated and analysed by SDS-PAGE. When DSP cross-linked thymocyte material was immunoprecipitated with either anti-ThB or anti-Ly 5 monoclonal antibodies, and then cleaved, molecules with masses identical to Ly 5 (Mr180 kD) and ThB (Mr 16–18 kD) were obtained. However, if the cross-linker was not cleaved, the intact product had a molecular mass of 〉 200 kD. The identity of these co-precipitated, cross-linked moieties was formally proved by limited proteolysis peptide map analysis. The data indicated that the ThB and Ly 5 antigens were associated on the thymocyte cell surface but no such association could be found on peripheral lymphocytes. The ThB-Ly 5 interaction may indicate an association relevant to the differentiation of thymocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.1989.tb00445.x
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  • 2
    Electronic Resource
    Electronic Resource
    THE CELL SURFACE PHENOTYPE OF MOUSE NEUTROPHILS (1985)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 12 (1985), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Using monoclonal antibodies, mouse peritoneal neutrophils were typed for the presence of 23 cell surface alloantigens, the expression of which was quantitated by flow cytofluorometry and compared with that of lymphocytes. The H-2K and H-2D alloantigens and β2-microglobulin were present on all neutrophils, but Ia and Qa antigens were not detected. It was found that Ly-5.1, Ly-15.2, Ly-21.2, Ly-24.2 (Pgp-1) and Ly-25.1 were present on 〉90% of neutropils; Ly-6.2 and Ly-27.2 were absent, but Ly-28.2 (encoded by an Ly-6 linked gene), was present on 〉90% of neutrophils. As expected, the lymphocyte-specific antigens Ly-1.1, Ly-2.2, Ly-3.1, Ly-7.2, Ly-12.1 and Thy-1.2 were absent from the neutrophils. When compared with lymphocytes, marked differences in alloantigen expression on neutrophils were seen for Ly-5.1, Ly-24.2 and Ly-28.2. These studies should be of value in the study of neutrophil structure and function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.1985.tb00852.x
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  • 3
    Electronic Resource
    Electronic Resource
    HUMAN ‘Ia’ ANTIGEN POPULATIONS DEFINED BY MONOCLONAL ANTIBODIES (1983)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 10 (1983), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The relationships between the antigens recognized by four monoclonal anti-human ‘Ia’-like antibodies were investigated using sequential immunoprecipitation and capping techniques. Two of the antibodies were ‘monomorphic’ and have previously been shown to recognize epitopes in which carbohydrate residues are involved, whereas the two ‘polymorphic’ antibodies recognized protein-defined epitopes—one of these epitopes being present on MB+DR- molecules. In the absence of an indisputable anti-DR monoclonal antibody, it was not possible to conclusively verify which ‘Ia’-encoded antigens were detected by the anti-‘Ia’-like monoclonal antibodies. Nevertheless, several firm conclusions could be drawn: (a) so-called ‘monomorphic’ antibodies do not necessarily react with all ‘Ia’ molecules encoded by a single locus—from the results using the two monomorphic antibodies, B5.1 and 3F1.1, described herein, two populations of antigens being B5.1+3F1.1+ and B5.1+3F1.1- were identified; (b) cross-reactivity of a polymorphic determinant expressed on antigenically-separable ‘Ia’ molecules was noted—using the two polymorphic antibodies, 26.1 and F5C9, molecules which were 26.1+F5C9+ and 26.1-F5C9+ were identified; and (c) the data clearly point to the existence of at least two loci coding for ‘Ia’-like antigens (one of which may or may not be the HLA-DR locus). Given that polymorphisms can now include protein- and carbohydrate-defined epitopes, that cross-reactions occur and that the definition of DR itself by monoclonal antibodies is not clear, the complexity of the human ‘Ia’ antgens is apparent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.1983.tb00793.x
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  • 4
    Electronic Resource
    Electronic Resource
    LY - AND ECOTROPIC MuLV ANTIGENS ARE SEPARTE ENTITIES (1982)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 9 (1982), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Murine alloantisera to lymphocyte (Ly) alloantigens also contain antibodies to murine leukaemia viruses (MuLV) in high titre. A number of such sera were extensively absorbed with ecotropic AKR murine leukaemia virus to remove contaqiminating anti-MuLV antibodies. The virus-absorbed and unabsorbed antisera wre then examined by the radioimmune precipitation (RIP) assay for anti-MuLV antibodies, and for anti-Ly antibodies by cytotoxicity assays against specific target cells. The results presented here demonstrate that whilst virus absorption markdely reduced the anti-viraql titres of the alloantisera they ahd no effect on the anti-Ly titres of any of them. This indicated that none of the Ly antigens which was examined cross reacts with the antigens of ecotropic AKR-MuLV and further suggest that Ly antigens are not encoded by the genes of endogenous ecotropic MuLVs. It was also demonstrated that the majority of the constaninating anti-MuLV antibodies present in anti-Ly-6.2 proteins of the virus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.1982.tb01004.x
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  • 5
    Electronic Resource
    Electronic Resource
    THE DETECTION OF PUBLIC Ia ANTIGENS ON THE SURFACE OF B6-C.H-2b m 1 2 BLAST CELLS (1982)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 9 (1982), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The B6-C.H-2bm12 (bm12) strain has previously been described as a spontaneously occurring mutant wherein the parental C57BL/6 Iab specificities (Ia.3, 8, 9, 15 and 20) are absent or reduced in amount. To further characterize the bm12 mutant the antigenic phenotype of LPS-activated bm12 blast cells was determined with monoclonal and conventional anti-Ia antibodies. By direct testing and absorption the public Ia.8, 9 and 15 specificities were readily detected on both parental C57BL/6 and mutant bm12 blast cells, and the amounts of these antigens on activated bm12 cells were similar to or less than those found on C57BL/6 blast cells. The findings demonstrate that the Ia-1 mutation has not resulted in an absolute loss of these specificities, but has altered their expression, or their accessibility to antibody on resting cells. By contrast, the private Ia. 20 specificity and another specificity (designated Iab) could not be detected on bm12 blast cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.1982.tb00978.x
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  • 6
    Electronic Resource
    Electronic Resource
    CELL MEMBRANE ALLOANTIGENIC DETERMINANTS OF SEVERAL UNUSUAL MOUSE STRAINS (1980)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 7 (1980), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Several interesting and clinically important strains of Mus musculus were serotyped with alloantisera recognizing the different alleles of the Thy-1, Ly-1, Ly-2, Ly-3, Ly-4, Ly-5, Ly-6 and Ly-7 loci. The strains were the Biozzi high and low responder strains, of importance in immune responsiveness studies, and the strains BXSB, MRL and MRL—lpr/lpr—strains which spontaneously develop immune complex disease. In addition the related species Mus musculus castaneus was typed with the same reagents. The knowledge of the cell surface phenotype should prove useful in various functional studies involving these interesting strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.1980.tb00733.x
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  • 7
    Electronic Resource
    Electronic Resource
    GENETIC AND BIOCHEMICAL CHARACTERIZATION OF ANTIGENS ENCODED BY THE LY-24 (Pgp-1) LOCUS1 (1987)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 14 (1987), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Three allele-specific monoclonal antibodies to Pgp-1 (Ly-24) were used to biochemically characterize the cell surface structures with which they reacted and to map the gene(s) coding for these antigens. The targets of these three monoclonal antibodies (mAb) were shown to be encoded by a gene situated on chromosome 2 close to β2m [gene order (Pgp-1-β2m-a)] and no recombination between the loci detected by the three antibodies was revealed by genetic analysis. The genetic mapping of loci and tissue distribution of these antigens suggested that they might all correspond to a particular allelic form of the mouse phagocyte glycoprotein-1 (Pgp-1) antigen. Biochemical and serological analysis confirmed that this was indeed the case and revealed that all three mAbs were directed to one epitope. It is surprising that the tissue distribution defined by one mAb (Ly-24A) was different from that for the two other (Ly-24B) antibodies, despite the serological and biochemical identity of their respective targets. The possible reason for this unusual finding is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.1987.tb00362.x
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  • 8
    Electronic Resource
    Electronic Resource
    Qa ANTIGENS AND THEIR DIFFERENTIAL DISTRIBUTION ON LYMPHOID, MYELOID AND STEM CELLS (1984)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 11 (1984), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.1984.tb00814.x
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  • 9
    Electronic Resource
    Electronic Resource
    PRESSURE-INDUCED SOLUBILIZATION OF MYOFIBRILLAR PROTEINS (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 41 (1976), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In an earlier study it was found that the application of high pressure to homogenates of meat in saline solution could increase water-holding capacity and promote the solubilization of proteins. These studies have now been extended to myofibrils prepared from sheep semimembrano-sus muscle. The amount of protein solubilized by pressurization was found to be dependent on (1) temperature, generally being greater at 0°C than at 30°C; (2) the nature and the concentration of the salt; and (3) the pH. SDS gel electrophoresis indicated that solubilization of all the major constituent proteins of the myofibril could be promoted. It is suggested that the imidazole group is implicated in the pressure solubilization effect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1976.tb01191.x
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  • 10
    Electronic Resource
    Electronic Resource
    An antigenic difference between cells forming early and late haematopoietic spleen colonies (CFU-S) (1984)
    [s.l.] : Nature Publishing Group
    Nature 307 (1984), S. 638-641 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A cytotoxic IgG2b monoclonal antibody to a Oa-2 like' alloantigen, Qa-m2 (rf. 4), reacts with a minority ClO-15%) of bone marrow cells4 (mostly lymphocytes5) and has been shown to kill, in the presence of complement, megakaryocyte colony-forming cells (MEG-CFC) but not granulocyte-macrophage ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/307638a0
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