Publication Date:
2007-04-28
Description:
Dynamin 1 is a neuron-specific guanosine triphosphatase thought to be critically required for the fission reaction of synaptic vesicle endocytosis. Unexpectedly, mice lacking dynamin 1 were able to form functional synapses, even though their postnatal viability was limited. However, during spontaneous network activity, branched, tubular plasma membrane invaginations accumulated, capped by clathrin-coated pits, in synapses of dynamin 1-knockout mice. Synaptic vesicle endocytosis was severely impaired during strong exogenous stimulation but resumed efficiently when the stimulus was terminated. Thus, dynamin 1-independent mechanisms can support limited synaptic vesicle endocytosis, but dynamin 1 is needed during high levels of neuronal activity.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ferguson, Shawn M -- Brasnjo, Gabor -- Hayashi, Mitsuko -- Wolfel, Markus -- Collesi, Chiara -- Giovedi, Silvia -- Raimondi, Andrea -- Gong, Liang-Wei -- Ariel, Pablo -- Paradise, Summer -- O'toole, Eileen -- Flavell, Richard -- Cremona, Ottavio -- Miesenbock, Gero -- Ryan, Timothy A -- De Camilli, Pietro -- CA46128/CA/NCI NIH HHS/ -- D.061/Telethon/Italy -- DA018343/DA/NIDA NIH HHS/ -- DA17297/DA/NIDA NIH HHS/ -- DK45735/DK/NIDDK NIH HHS/ -- NS036942/NS/NINDS NIH HHS/ -- NS36251/NS/NINDS NIH HHS/ -- P30 DA018343/DA/NIDA NIH HHS/ -- R01 NS036942/NS/NINDS NIH HHS/ -- RR-000592/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 2007 Apr 27;316(5824):570-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Kavli Institute for Neuroscience, Yale University School of Medicine, New Haven, CT 06510, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17463283" target="_blank"〉PubMed〈/a〉
Keywords:
Action Potentials
;
Animals
;
Cell Membrane/ultrastructure
;
Clathrin-Coated Vesicles/metabolism/ultrastructure
;
Dynamin I/genetics/*physiology
;
Dynamin II
;
Dynamin III/physiology
;
Electric Stimulation
;
*Endocytosis
;
Excitatory Postsynaptic Potentials
;
Exocytosis
;
Inhibitory Postsynaptic Potentials
;
Mice
;
Mice, Knockout
;
Microscopy, Electron
;
Neurons/*physiology/ultrastructure
;
Patch-Clamp Techniques
;
Presynaptic Terminals/physiology/ultrastructure
;
Synapses/*physiology/ultrastructure
;
Synaptic Transmission
;
Synaptic Vesicles/*physiology/ultrastructure
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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