Publication Date:
1995-07-07
Description:
Many proteins are associated with the outer layer of the cell membrane through a posttranslationally added glycosyl phosphatidylinositol (GPI) anchor. The functional significance of this type of protein linkage is unclear, although it results in increased lateral mobility, sorting to the apical surface of the cell, reinsertion into cell membranes, and possibly cell signaling. Here evidence is presented that GPI-linked proteins can undergo intermembrane transfer in vivo. GPI-linked proteins expressed on the surface of transgenic mouse red blood cells were transferred in a functional form to endothelial cells in vivo. This feature of GPI linkage may be potentially useful for the delivery of therapeutic proteins to vascular endothelium.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kooyman, D L -- Byrne, G W -- McClellan, S -- Nielsen, D -- Tone, M -- Waldmann, H -- Coffman, T M -- McCurry, K R -- Platt, J L -- Logan, J S -- HL 46810/HL/NHLBI NIH HHS/ -- HL 50985/HL/NHLBI NIH HHS/ -- HL 52297/HL/NHLBI NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1995 Jul 7;269(5220):89-92.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Sir William Dunn School of Pathology, Oxford, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7541557" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigens, CD/genetics/*metabolism
;
Antigens, CD55
;
Antigens, CD59
;
Base Sequence
;
Bone Marrow Transplantation
;
Cell Membrane/metabolism
;
Cells, Cultured
;
Complement Inactivator Proteins/genetics/*metabolism
;
Endothelium, Vascular/cytology/*metabolism
;
Erythrocytes/*metabolism
;
Globins/genetics
;
Glycosylphosphatidylinositols/*metabolism
;
Humans
;
Membrane Glycoproteins/genetics/*metabolism
;
Mice
;
Mice, Transgenic
;
Molecular Sequence Data
;
Myocardium/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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