Publication Date:
1991-08-16
Description:
Pit-1, a tissue-specific POU domain transcription factor, is required for the activation of the prolactin, growth hormone, and Pit-1 promoters that confer regulation by epidermal growth factor, adenosine 3',5'-monophosphate (cAMP), and phorbol esters. Pit-1 is phosphorylated in pituitary cells at two distinct sites in response to phorbol esters and cAMP. Phosphorylation of Pit-1 modifies its conformation on DNA recognition elements and results in increased binding at certain sites and decreased binding at other sites, dependent on DNA sequences adjacent to the core Pit-1 binding motif. One residue (Thr220), located in the POU homeodomain within a sequence conserved throughout the POU-domain family, confers these responses.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kapiloff, M S -- Farkash, Y -- Wegner, M -- Rosenfeld, M G -- DK 18477/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 1991 Aug 16;253(5021):786-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Eukaryotic Regulatory Biology Program, School of Medicine, University of California, San Diego, La Jolla 92093-0648.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1652153" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Base Sequence
;
Binding Sites
;
Cell Line
;
Cyclic AMP/pharmacology
;
DNA/metabolism
;
DNA-Binding Proteins/chemistry/*physiology
;
In Vitro Techniques
;
Molecular Sequence Data
;
Peptide Mapping
;
Phosphorylation
;
Phosphothreonine/metabolism
;
Pituitary Gland/*physiology
;
Protein Kinases/metabolism
;
Regulatory Sequences, Nucleic Acid
;
Structure-Activity Relationship
;
Tetradecanoylphorbol Acetate/pharmacology
;
Transcription Factor Pit-1
;
Transcription Factors/chemistry/*physiology
;
Trypsin
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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