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  • 1
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    A NAC transcription factor and SNI1 cooperatively suppress basal pathogen resistance in Arabidopsis thaliana (2012)
    Oxford University Press
    Details
    Publication Date: 2012-10-10
    Description: Transcriptional repression of pathogen defense-related genes is essential for plant growth and development. Several proteins are known to be involved in the transcriptional regulation of plant defense responses. However, mechanisms by which expression of defense-related genes are regulated by repressor proteins are poorly characterized. Here, we describe the in planta function of CBNAC, a calmodulin-regulated NAC transcriptional repressor in Arabidopsis . A T-DNA insertional mutant ( cbnac1 ) displayed enhanced resistance to a virulent strain of the bacterial pathogen Pseudomonas syringae DC3000 ( Pst DC3000), whereas resistance was reduced in transgenic CBNAC overexpression lines. The observed changes in disease resistance were correlated with alterations in pathogenesis-related protein 1 ( PR1 ) gene expression. CBNAC bound directly to the PR1 promoter. SNI1 ( suppressor of nonexpressor of PR genes1, inducible 1 ) was identified as a CBNAC-binding protein. Basal resistance to Pst DC3000 and derepression of PR1 expression was greater in the cbnac1 sni1 double mutant than in either cbnac1 or sni1 mutants. SNI1 enhanced binding of CBNAC to its cognate PR1 promoter element. CBNAC and SNI1 are hypothesized to work as repressor proteins in the cooperative suppression of plant basal defense.
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
    Published by Oxford University Press
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  • 2
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    Oligonucleotide-modified gold nanoparticles for intracellular gene regulation (2006)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2006-05-20
    Description: We describe the use of gold nanoparticle-oligonucleotide complexes as intracellular gene regulation agents for the control of protein expression in cells. These oligonucleotide-modified nanoparticles have affinity constants for complementary nucleic acids that are higher than their unmodified oligonucleotide counterparts, are less susceptible to degradation by nuclease activity, exhibit greater than 99% cellular uptake, can introduce oligonucleotides at a higher effective concentration than conventional transfection agents, and are nontoxic to the cells under the conditions studied. By chemically tailoring the density of DNA bound to the surface of gold nanoparticles, we demonstrated a tunable gene knockdown.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rosi, Nathaniel L -- Giljohann, David A -- Thaxton, C Shad -- Lytton-Jean, Abigail K R -- Han, Min Su -- Mirkin, Chad A -- New York, N.Y. -- Science. 2006 May 19;312(5776):1027-30.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry and International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113 USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16709779" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cell Line ; Deoxyribonucleases/metabolism ; *Gene Expression Regulation ; Glutathione/metabolism ; *Gold ; Green Fluorescent Proteins/genetics ; HeLa Cells ; Humans ; Mice ; *Nanostructures ; *Oligodeoxyribonucleotides, Antisense/metabolism ; RNA, Messenger
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 3
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    JNK expression by macrophages promotes obesity-induced insulin resistance and inflammation (2012)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2012-12-12
    Description: The cJun NH(2)-terminal kinase (JNK) signaling pathway contributes to inflammation and plays a key role in the metabolic response to obesity, including insulin resistance. Macrophages are implicated in this process. To test the role of JNK, we established mice with selective JNK deficiency in macrophages. We report that feeding a high-fat diet to control and JNK-deficient mice caused similar obesity, but only mice with JNK-deficient macrophages remained insulin-sensitive. The protection of mice with macrophage-specific JNK deficiency against insulin resistance was associated with reduced tissue infiltration by macrophages. Immunophenotyping demonstrated that JNK was required for pro-inflammatory macrophage polarization. These studies demonstrate that JNK in macrophages is required for the establishment of obesity-induced insulin resistance and inflammation.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3835653/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3835653/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Han, Myoung Sook -- Jung, Dae Young -- Morel, Caroline -- Lakhani, Saquib A -- Kim, Jason K -- Flavell, Richard A -- Davis, Roger J -- CA065861/CA/NCI NIH HHS/ -- DK032520/DK/NIDDK NIH HHS/ -- DK080756/DK/NIDDK NIH HHS/ -- DK090963/DK/NIDDK NIH HHS/ -- DK093000/DK/NIDDK NIH HHS/ -- R01 CA065861/CA/NCI NIH HHS/ -- R01 DK080756/DK/NIDDK NIH HHS/ -- R24 DK090963/DK/NIDDK NIH HHS/ -- U24 DK093000/DK/NIDDK NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2013 Jan 11;339(6116):218-22. doi: 10.1126/science.1227568. Epub 2012 Dec 6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Worcester, MA 01605, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23223452" target="_blank"〉PubMed〈/a〉
    Keywords: Adipose Tissue/immunology/pathology ; Animals ; Diet, High-Fat ; Glucose Clamp Technique ; Immunophenotyping ; Inflammation/immunology/*physiopathology ; *Insulin Resistance ; Islets of Langerhans/pathology ; MAP Kinase Signaling System ; Macrophage Activation ; Macrophages/*enzymology/*immunology/physiology ; Mice ; Mitogen-Activated Protein Kinase 8/deficiency/genetics/*metabolism ; Mitogen-Activated Protein Kinase 9/deficiency/genetics/*metabolism ; Obesity/immunology/*physiopathology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 4
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    Secondary metabolite symbiosis [Microbiology] (2012)
    National Academy of Sciences
    Details
    Publication Date: 2012-12-12
    Description: Secondary metabolites are ubiquitous in bacteria, but by definition, they are thought to be nonessential. Highly toxic secondary metabolites such as patellazoles have been isolated from marine tunicates, where their exceptional potency and abundance implies a role in chemical defense, but their biological source is unknown. Here, we describe the...
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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  • 5
    Electronic Resource
    Electronic Resource
    Dependence of the optical properties of p-CdTe and p-Cd0.96Zn0.04Te on the bromine concentration in a bromine-methanol etching solution (1993)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 73 (1993), S. 4074-4076 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Photoluminescence measurements were carried out to investigate the dependence of the optical properties of p-CdTe and p-Cd0.96Zn0.04Te on various relative concentrations of bromine in a mixture of methanol and bromine. As the bromine concentration increased, the intensity of the luminescence increased; additionally, an exciton bound to a neutral acceptor (A°X) peak at 1.588 eV in p-CdTe was resolved into an exciton bound to neutral donor (D°X) peak at 1.592 eV and an A°X peak. The mixed bands of the 1.574 and 1.546 eV peaks were well resolved by using an etching bromine concentration of 2%; in particular, the intensities of the D°X and 1.574 eV peaks increased as much as five and seven times, respectively. The intensity of the peak at 1.48 eV related to defects also increased. The intensity of A°X at 1.609 eV in p-Cd0.96Zn0.04Te changed slightly with the bromine concentration. The intensities of the luminescence peaks due to the recombination of the electrons in conduction band with acceptors and to the defect relation did not vary. These results indicated that the number of Cd vacancies could be reduced due to the addition of Zn.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.352832
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  • 6
    Electronic Resource
    Electronic Resource
    Strain effects in CdTe/Si heterostructures (1997)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 82 (1997), S. 6012-6015 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Photoluminescence (PL) and spectroscopic ellipsometry measurements on CdTe/Si strained heterostructures grown by molecular beam epitaxy were carried out to investigate the effect of the strain and the dependence of the strain on the Si tilted substrates. The results of the PL spectra showed that the relative intensity ratio between the peak at 1.452 eV and the bound-exciton peak for the CdTe epilayer grown on the Si (100) 1° tilted substrate had a minimum value and that the strain for the CdTe epilayer grown on the Si (100) 8° tilted substrate had a minimum value. When rapid thermal annealing (RTA) was performed at 55 °C, the PL spectra showed that the relative intensity ratio between the peak at 1.452 eV and that at 1.574 eV for the CdTe epilayer grown on the Si (100) 8° tilted substrate had a minimum value and that the strain for the CdTe epilayer grown on the Si (100) 1° tilted substrate had a minimum value. Spectroscopic ellipsometry measurements showed that the spectrum of the dielectric constant of the CdTe epilayer grown on the Si (100) 8° tilted substrate is similar to that of the CdTe bulk. These results indicate that the strains in the CdTe layers grown on Si substrates are strongly dependent on the Si substrate orientation and that the crystallinity of the CdTe epitaxial layer grown on the Si substrate is remarkably improved by RTA. © 1997 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.366467
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  • 7
    Electronic Resource
    Electronic Resource
    Codoping characteristics of Zn with Mg in GaN (2000)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 77 (2000), S. 1123-1125 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The doping characteristics of Mg–Zn codoped GaN films grown by metalorganic chemical vapor deposition are investigated. By means of the concept of Mg–Zn codoping technique, we have grown p-GaN showing a low electrical resistivity (0.72 Ω cm) and a high hole concentration (8.5×1017 cm−3) without structural degradation of the film. It is thought that the codoping of Zn atoms with Mg raises the Mg activation ratio by reducing the hydrogen solubility in p-GaN. In addition, the measured specific contact resistance of Mg–Zn codoped GaN film is 5.0×10−4 Ω cm2, which is one order of magnitude lower than that of Mg doped only GaN film (1.9×10−3 Ω cm2). © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1289494
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  • 8
    Electronic Resource
    Electronic Resource
    THE USE OF COMPLIANCE TO INVESTIGATE FATIGUE CRACK RETARDATION BY COVER PLATES (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Fatigue & fracture of engineering materials & structures 13 (1990), S. 0 
    Details
    ISSN: 1460-2695
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract— This paper develops the compliance approach to the problem of load sharing between a cracked plate and a cover plate used to bridge the crack. The theory is validated by using calculated stress intensity factors for the covered and uncovered cases to reduce experimentally observed growth rates to a common base. Calculations are then made on the effect of cover plate width on fatigue crack retardation in order to demonstrate the predictive capability of the technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1460-2695.1990.tb00628.x
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  • 9
    Electronic Resource
    Electronic Resource
    Transovarial transmission of two microsporidia in the silkworm, Bombyx mori, and disease occurrence in the progeny population
    Amsterdam : Elsevier
    Journal of Invertebrate Pathology 51 (1988), S. 41-45 
    Details
    ISSN: 0022-2011
    Keywords: Bombyx mori ; Microsporidia ; Nosema bombycis ; Nosema sp. M11 ; immunofluorescent identification ; transovarial transmission
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0022-2011(88)90086-9
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  • 10
    Electronic Resource
    Electronic Resource
    Determination of trace elements in high purity copper by INAA, GFAAS and ICP/AES (1998)
    Springer
    Journal of radioanalytical and nuclear chemistry 234 (1998), S. 77-83 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Trace impurity elements in high purity copper metal (4 mine class) put on the market were analyzed by Instrumental Neutron Activation Analysis (INAA) and the results compared with those from Graphite Furnace Atomic Absorption Spectrophotometry (GFAAS) and Inductively Coupled Plasma Atomic Emission Spectrophotometry (ICP-AES). The sample irradiation was done at the irradiation facilities (thermal neutron flux, 5·1012 n·cm−2·s−1) of the TRIGA Mark-III research reactor in the Korea Atomic Energy Research Institute. Four unalloyed copper standards (NIST SRM # 393, 394, 395 and 398) were used to identify the accuracy and precision of the analytical procedure. The homogeneity of samples was assessed by means of the elements such as Ag, As, Co, Sb, Se and Zn. The analytical results of INAA, GFAAS and ICP-AES were in good agreement within expected uncertainties each other and showed the possibility of using them for the analytical quality control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02389751
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