Publication Date:
2011-02-19
Description:
The mechanistic basis of resistance of vertebrate populations to contaminants, including Atlantic tomcod from the Hudson River (HR) to polychlorinated biphenyls (PCBs), is unknown. HR tomcod exhibited variants in the aryl hydrocarbon receptor 2 (AHR2) that were nearly absent elsewhere. In ligand-binding assays, AHR2-1 protein (common in the HR) was impaired as compared to widespread AHR2-2 in binding TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) and in driving expression in reporter gene assays in AHR-deficient cells treated with TCDD or PCB126. We identified a six-base deletion in AHR2 as the basis of resistance and suggest that the HR population has undergone rapid evolution, probably due to contaminant exposure. This mechanistic basis of resistance in a vertebrate population provides evidence of evolutionary change due to selective pressure at a single locus.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246799/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246799/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wirgin, Isaac -- Roy, Nirmal K -- Loftus, Matthew -- Chambers, R Christopher -- Franks, Diana G -- Hahn, Mark E -- ES00260/ES/NIEHS NIH HHS/ -- P30 ES000260/ES/NIEHS NIH HHS/ -- P30 ES000260-47S2/ES/NIEHS NIH HHS/ -- P42 ES007381/ES/NIEHS NIH HHS/ -- P42 ES007381-15S5/ES/NIEHS NIH HHS/ -- P42 ES010344/ES/NIEHS NIH HHS/ -- P42 ES010344-05S2/ES/NIEHS NIH HHS/ -- P42ES007381/ES/NIEHS NIH HHS/ -- R01 ES015447-03/ES/NIEHS NIH HHS/ -- R01ES015447/ES/NIEHS NIH HHS/ -- New York, N.Y. -- Science. 2011 Mar 11;331(6022):1322-5. doi: 10.1126/science.1197296. Epub 2011 Feb 17.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Environmental Medicine, New York University School of Medicine,Tuxedo, NY 10987, USA. Isaac.wirgin@nyumc.org〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21330491" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Line, Tumor
;
DNA, Mitochondrial/genetics
;
Drug Resistance
;
Evolution, Molecular
;
Gadiformes/*genetics/*metabolism
;
Gene Expression
;
Gene Frequency
;
Genes, Reporter
;
Haplotypes
;
Ligands
;
Mice
;
Molecular Sequence Data
;
New York
;
Polychlorinated Biphenyls/metabolism/*toxicity
;
Polymorphism, Genetic
;
Polymorphism, Single Nucleotide
;
Receptors, Aryl Hydrocarbon/chemistry/*genetics/*metabolism
;
Rivers
;
Selection, Genetic
;
Sequence Deletion
;
Tetrachlorodibenzodioxin/metabolism/*toxicity
;
Water Pollutants, Chemical/metabolism/*toxicity
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink