Publication Date:
2004-07-17
Description:
Interaction of regulatory DNA binding proteins with their target sites is usually preceded by binding to nonspecific DNA. This speeds up the search for the target site by several orders of magnitude. We report the solution structure and dynamics of the complex of a dimeric lac repressor DNA binding domain with nonspecific DNA. The same set of residues can switch roles from a purely electrostatic interaction with the DNA backbone in the nonspecific complex to a highly specific binding mode with the base pairs of the cognate operator sequence. The protein-DNA interface of the nonspecific complex is flexible on biologically relevant time scales that may assist in the rapid and efficient finding of the target site.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kalodimos, Charalampos G -- Biris, Nikolaos -- Bonvin, Alexandre M J J -- Levandoski, Marc M -- Guennuegues, Marc -- Boelens, Rolf -- Kaptein, Robert -- GM 23467/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2004 Jul 16;305(5682):386-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, Netherlands.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15256668" target="_blank"〉PubMed〈/a〉
Keywords:
Bacterial Proteins/*chemistry/*metabolism
;
Base Pairing
;
Binding Sites
;
DNA, Bacterial/*chemistry/*metabolism
;
Diffusion
;
Dimerization
;
Escherichia coli/chemistry/genetics/metabolism
;
Escherichia coli Proteins/chemistry/metabolism
;
Hydrogen Bonding
;
Lac Repressors
;
Models, Molecular
;
Nuclear Magnetic Resonance, Biomolecular
;
Nucleic Acid Conformation
;
Operator Regions, Genetic
;
Protein Binding
;
Protein Conformation
;
Protein Structure, Tertiary
;
Repressor Proteins/*chemistry/*metabolism
;
Static Electricity
;
Thermodynamics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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