Publication Date:
1990-06-29
Description:
The TATA binding protein, TFIID, plays a central role in the initiation of eukaryotic mRNA synthesis. Here, we present a human cDNA clone for this factor. Comparison of its predicted protein sequence with those from Drosophila and yeast reveals a highly conserved carboxyl-terminal 180 amino acids. By contrast, the amino-terminal region of TFIID has diverged in both sequence and length. A striking feature of the human protein is a stretch of 38 glutamine residues in the NH2-terminal region. Expression of human TFIID in both Escherichia coli and HeLa cells produces a protein that binds specifically to a TATA box and promotes basal transcription; the conserved COOH-terminal portion of the protein is sufficient for both of these activities. Recombinant TFIID forms a stable complex on a TATA box either alone or in combination with either of the general transcription factors, TFIIA or TFIIB. Full-length recombinant TFIID is able to support Sp1 activated transcription in a TFIID-depleted nuclear extract, while a deletion of the NH2-terminal half of the protein is not. These results indicate the importance of the NH2-terminal region for upstream activation functions and suggest that additional factors (co-activators) are required for mediating interactions with specific regulators.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Peterson, M G -- Tanese, N -- Pugh, B F -- Tjian, R -- New York, N.Y. -- Science. 1990 Jun 29;248(4963):1625-30.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2363050" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Base Sequence
;
Cell Nucleus/metabolism
;
Cloning, Molecular/methods
;
DNA/genetics
;
DNA, Neoplasm/genetics
;
*Gene Expression Regulation
;
Glutamine
;
HeLa Cells/metabolism
;
Humans
;
Molecular Sequence Data
;
Oligonucleotide Probes
;
*Promoter Regions, Genetic
;
RNA, Messenger/genetics
;
Recombinant Proteins/isolation & purification/metabolism
;
Transcription Factor TFIID
;
Transcription Factors/*genetics/isolation & purification/metabolism
;
*Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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