Publication Date:
2009-03-28
Description:
Protein folding in the endoplasmic reticulum is a complex process whose malfunction is implicated in disease and aging. By using the cell's endogenous sensor (the unfolded protein response), we identified several hundred yeast genes with roles in endoplasmic reticulum folding and systematically characterized their functional interdependencies by measuring unfolded protein response levels in double mutants. This strategy revealed multiple conserved factors critical for endoplasmic reticulum folding, including an intimate dependence on the later secretory pathway, a previously uncharacterized six-protein transmembrane complex, and a co-chaperone complex that delivers tail-anchored proteins to their membrane insertion machinery. The use of a quantitative reporter in a comprehensive screen followed by systematic analysis of genetic dependencies should be broadly applicable to functional dissection of complex cellular processes from yeast to human.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2877488/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2877488/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jonikas, Martin C -- Collins, Sean R -- Denic, Vladimir -- Oh, Eugene -- Quan, Erin M -- Schmid, Volker -- Weibezahn, Jimena -- Schwappach, Blanche -- Walter, Peter -- Weissman, Jonathan S -- Schuldiner, Maya -- 081671/Wellcome Trust/United Kingdom -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2009 Mar 27;323(5922):1693-7. doi: 10.1126/science.1167983.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94143, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19325107" target="_blank"〉PubMed〈/a〉
Keywords:
Endoplasmic Reticulum/*metabolism
;
Epistasis, Genetic
;
Gene Deletion
;
Gene Expression Regulation, Fungal
;
*Genes, Fungal
;
Genes, Reporter
;
Membrane Proteins/chemistry/genetics/metabolism
;
Mutation
;
Phenotype
;
*Protein Folding
;
*Protein Processing, Post-Translational
;
Protein Structure, Tertiary
;
Saccharomyces cerevisiae/*genetics/metabolism
;
Saccharomyces cerevisiae Proteins/*chemistry/genetics/metabolism
;
Secretory Pathway
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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