ISSN:
1600-5767
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Geosciences
,
Physics
Notes:
Protein crystals have been cross-linked by a gentle technique whereby glutaraldehyde is introduced by vapor diffusion into the crystallization droplet containing the crystals. Diffraction analyses of crystals of three different proteins show that cross-linking prevents, in a large part, the lattice disorder normally observed on rapid cooling of these crystals. The diffraction results suggest that this cross-linking procedure, performed as a simple extension of the standard vapor-diffusion crystallization experiment, may generally aid in the cooling of fragile protein crystals for which standard procedures of cryopreservation prove inadequate.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S002188989801053X
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