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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Macromolecules 27 (1994), S. 3666-3668 
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 2
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    Canberra : Periodicals Archive Online (PAO)
    Quarterly review of the rural economy. 8:3 (1955:July) 127 
    ISSN: 0156-7446
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Economics
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Methods in cell science 13 (1991), S. 275-283 
    ISSN: 1573-0603
    Keywords: cryopreservation ; embryonic ; chick ; myogenic stem cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Undifferentiated embryonic chick stem cells provide a good in vitro test system to examine the effects of recombinant growth factors on the resultant phenotypic expression of these cells. One of the major difficulties in using freshly isolated cells is variation in the proportion of stem cells recovered from different cell harvests. Variation is of particular concern when multiple cell harvests are necessary to provide cells for assaying a single growth factor. As an attempt to minimize this variation, we have examined the efficacy of cryopreserving myogenic lineage-committed stem cells derived from embryonic chick leg muscle. Percent recovery, viability, differentiation morphology, and cellular proliferation rates were compared between sister cultures of freshly isolated and cryopreserved stem cells. Embryonic chick stem cells retained their capacity to differentiate myogenically in culture when slowly frozen in and recovered from 7.5% dimethyl sulfoxide medium supplemented with horse serum and stage-specific embryo extract.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Methods in cell science 14 (1992), S. 31-36 
    ISSN: 1573-0603
    Keywords: cell culture ; antibody ; HRP ; visualization ; DNA analysis ; phenotypic expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The current study outlines a procedure, designated enzyme-linked immuno-culture assay (ELICA), that will measure phenotypic expression of cultured cells in small plate assays. Given standard curves for phenotypic expression markers and in situ DNA analysis, this procedure will quantitate (to nanogram levels) intracellular, cell surface, or extracellular phenotypic expression markers; visualize the location of the markers; and determine DNA content, all within the same well of a 24- or 96-well tissue culture plate.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-0603
    Keywords: isolation ; myosatellite stem cells ; pluripotent stem cells ; myogenic ; uncommitted ; chick
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The current study outlines the isolation and culture of two populations of cells derived from Day 11 embryonic chick leg muscle and associated connective tissues. The two populations consisted of myogenic lineage-committed stem cells (myosatellite stem cells) and lineage-uncommitted stem cells (pluripotent stem cells). After long-term culture the lineage-uncommitted stem cell population displayed differentiated phenotypes suggestive of the following adult tissues, fibroblasts, muscle, fat, cartilage, and bone.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Methods in cell science 17 (1995), S. 17-23 
    ISSN: 1573-0603
    Keywords: Defined media ; Growth factors ; Mitogenesis ; Plating ; Serum containing media ; Serum-free media ; Stage 24 chick cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A modification of the standard plating of Stage 24 chick limb bud cells in serum-containing media (SCM) is described in which the cells are incubated in SCM for 3 hours before direct plating in a serum-free chemically defined media (SFM). This results in equal plating efficiencies to those obtained with plating in SCM and may allow for more defined culture conditions when testing mitogenic growth factors found in serum.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-904X
    Keywords: dry powder aerosol ; inhalation ; powder flow ; low density ; salbutamol sulphate ; L-leucine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Pharmaceutical research 15 (1998), S. 562-569 
    ISSN: 1573-904X
    Keywords: dry powder aerosol ; inhalation ; protein ; mixing ; ternary component ; polyethylene glycol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. To evaluate the use of carrier-based dry powder aerosols for inhalation delivery of proteins and examine the effect of fine particle excipients as potential formulation performance modifiers. Methods. Bovine serum albumin (BSA) was co-processed with malto-dextrin by spray-drying to produce model protein particles. Aerosol formulations were prepared by tumble mixing protein powders with α-lactose monohydrate (63−90 μm) or modified lactoses containing between 2.5 and 10% w/w fine particle lactose (FPL) or micronised polyethylene glycol 6000. Powder blends were characterised in terms of particle size distribution, morphology and powder flow. Formulation performance in Diskhaler® and Rotahaler® devices was investigated using a twin stage impinger operating at 60 1 min−1. Results. Inhalation performance of binary ordered mixes prepared using BSA-maltodextrin and lactose (63−90 μm) was improved by addition of FPL and micronised PEG 6000. For the addition of 5% w/w FPL the protein fine particle fraction (0.5−6.4 μm) using the Diskhaler® was increased from 31.7 ± 2.4% to 47.4 ± 2.2%. Inclusion of FPL and micronised PEG 6000 changed the bulk properties of inhalation powders and reduced powder flow but did not affect device emptying. Unexpectedly, improvements in performance were found to be independent of the order of addition of FPL to the ternary powder formulations. SEM studies revealed that this was probably the result of a redistribution of protein particles between the coarse carrier lactose component and added FPL during mixing. Conclusions. Fine particle excipients can be used to improve the performance of carrier-based protein dry powder aerosols. Mechanistically, enhancement of performance is proposed to result from a redistribution of protein particles from coarse carrier particles to the fine particle component in the ternary mix.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 23 (1989), S. 23-39 
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: A controlled-release delivery vehicle for water-soluble osteogenic proteins from demineralized bone matrix was constructed using purified type I collagen. The water-soluble proteins were isolated from a 4 M GdnHCl extract of bone matrix. Although the water-soluble proteins were capable of inducing cartilage formation in vitro, they were incapable of inducing cartilage or bone in vivo when implanted intramuscularly into mice in the absence of an appropriate delivery vehicle. The collagen-based delivery vehicle alone was also incapable of inducing osteogenesis in vivo. However, when the water-soluble proteins were incorporated into the delivery vehicle, the combination was capable of inducing cartilage and bone 76% of the time. These results demonstrate that it is possible to formulate a controlled-release delivery vehicles for soluble bioactive factors which upon release interact with local responsive cells.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Controlled release delivery vehicles for water-soluble osteogenic proteins from demineralized bovine bone matrix were constructed using polyanhydride polymers. The water-soluble proteins were isolated from a 4 M guanidine hydrochloride extract of bone matrix. The water-soluble proteins possessed Chondrogenic Stimulating Activity (CSA) when tested in stage 24 chick limb bud cell cultures, but were incapable of inducing cartilage or bone in vivo when implanted intramuscularly into mice by themselves. The polyanhydride polymers alone were also incapable of inducting ectopic cartilage or bone. However, when the water-soluble proteins were incorporated into the polymeric delivery vehicle, the combination was capable of inducing cartilage and bone up to 50% of the time. These results demonstrate that it is possible to use polyanhydride polymers as controlled-release delivery vehicles for soluble bioactive factors that interact with a local cell population.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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