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  • 1
    Electronic Resource
    Electronic Resource
    Recombination frequency and DNA content of the distal part of the second chromosome ofDrosophila hydei sturtevant (1982)
    Springer
    Cellular and molecular life sciences 38 (1982), S. 328-329 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Four visible markers, including a newly isolated one, have been cytologically mapped on the second chromosome ofDrosophila hydei. Both the frequency of recombination and the amount of DNA between these markers have been determined. From these data the coefficient of exchange has been calculated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01949372
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  • 2
    Electronic Resource
    Electronic Resource
    The activity of two heat shock loci of Drosophila hydei in tissue culture cells and salivary gland cells as analyzed by in situ hybridization of complementary DNA (1979)
    Springer
    Chromosoma 72 (1979), S. 281-291 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Complementary DNA was made to poly A+ nuclear or polysomal RNA isolated from heat shock tissue culture cells of Drosophila hydei. A number of loci other than the four major heat shock loci are labelled after in situ hybridization of these cDNA preparations, while solution hybridization indicated that only about 10% of the cDNA was specific for heat shocked cells. Removal of the fraction of cDNA which could react with 25° C RNA and subsequent in situ hybridization of heat shock specific cDNA indicated that locus 4–81 B, a major salivary gland heat shock locus, is also active at 25° C in tissue culture cells, while locus 4–85 B is specifically activated by heat shock in tissue culture cells. This latter locus is not seen to be clearly puffed in salivary glands, but was shown to be active in that tissue both by direct autoradiography of salivary gland chromosomes after 3H-uridine labeling and by hybridization of cDNA to chromosomal RNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331090
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  • 3
    Electronic Resource
    Electronic Resource
    In situ hybridization of nuclear and cytoplasmic RNA to locus 2-48BC in Drosophila hydei (1978)
    Springer
    Chromosoma 65 (1978), S. 199-212 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The maximum grain density over the “heat-shock” locus 2-48BC of Drosophila hydei polytene chromosomes obtained after in situ hybridization of nuclear RNA extracted from tissue culture cells labelled during incubation at 37° C is five times higher than that obtainable by using polysomal RNA isolated from the same cells. Furthermore, the addition of a large excess of unlabelled polysomal RNA reduced the amount of in situ hybridization of nuclear RNA by only 20% showing that nuclear 2-48BC RNA contains sequences not present in polysomal 2-48BC RNA. — The polysomal 2-48BC RNA is polyadenylated, as are the RNA sequences present in the polysomes complementary to the other two major “heat shock” loci 2-32A and 2-36A. Polyadenylated RNA, with an apparent size of 15S, complementary to locus 2-48BC is also found in the cytoplasm of D. hydei salivary glands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327617
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  • 4
    Electronic Resource
    Electronic Resource
    Rapid and high resolution detection of in situ hybridisation to polytene chromosomes using fluorochrome-labeled RNA (1981)
    Springer
    Chromosoma 84 (1981), S. 1-18 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Fluorochrome-labeled RNA allows the rapid detection of in situ hybrids without the need for long exposure times as in the autoradiographical hybridisation methods. Resolution is high because of the high resolving power of fluorescence microscopy. The application of a previously reported method for the hybrido-cytochemical detection of DNA sequences to polytene chromosomes of Drosophilia is described. — The specificity and sensitivity of the method are demonstrated by the hybridisation with polytene chromosomes of 1) rhodamine-labeled 5S RNA, to the 5S rRNA sites of D. melanogaster (56F) and D. hydei (23 B), 2) rhodamine-labeled RNA complementary to a plasmid containing histone genes, to the 39DE region of D. melanogaster, 3) rhodamine-labeled D. melanogaster tRNA species (Gly-3 and Arg-2), to their respective loci in D. melanogaster, 4) rhodamine-labeled RNA complementary to the insert of plasmid 232.1 containing part of a D. melanogaster heat shock gene from locus 87 C, to D. hydei heat shock locus 2-32A. In the latter instance it was possible to demonstrate the labeling of a double band which escaped unambiguous detection by autoradiography in the radioactive cytochemical hybridisation procedure because of the low topological resolution of autoradiograms. — The sensitivity of the fluorochrome-labeled RNA method is compared with the radioactive methods which use 3H- or 125I-labeled RNAs. The factors governing the sensitivity and the number of bound fluorochrome molecules to be expected are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293359
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  • 5
    Electronic Resource
    Electronic Resource
    The products of the “heat-shock” loci of Drosophila hydei (1978)
    Springer
    Chromosoma 66 (1978), S. 115-125 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Antibody was raised against total Drosophila hydei embryonic cellular protein with a molecular weight between 65,000 and 70,000 dalton. This antiserum reacted with the 70,000 MW “heat-shock” peptide found, in 35S labelled cell extracts of “heat-shocked” D. hydei tissue culture cells or salivary glands. — The antibody was coupled to Sepharose 4B and this material was used to absorb polysomes obtained from tissue culture cells incubated at 37° C in the presence of tritiated RNA precursors. The relative concentrations of various RNA species complementary to the “heat-shock” loci 2-32A, 2-36A, and 2-48C in either bound, non-bound, or total polysomal material was then determined by in situ hybridization. The RNA species complementary to locus 2-36A was found to be enriched in the bound polysomal material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00295134
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  • 6
    Electronic Resource
    Electronic Resource
    Rapid sequence divergence in a heat shock locus of Drosophila (1980)
    Springer
    Chromosoma 81 (1980), S. 271-280 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In situ hybridization of cRNA transcribed from cloned D. melanogaster heat shock sequences to D. hydei chromosomes has shown that the D. hydei locus 2–32 A corresponds to the D. melanogaster locus 87 A/C and the D. hydei locus 2–36 A to the D. melanogaster locus 95 D, while the D. hydei locus 4–81 B corresponds to the D. melanogaster locus 63 BC. No hybridization to D. hydei chromosomes was found with cRNA transcribed from a clone containing the αβ sequences encoded by the D. melanogaster locus 87 C. Neither D. melanogaster heat shock RNA nor D. virilis heat shock RNA hybridized significantly to the D. hydei heat shock locus 2–48 B. Furthermore, D. hydei heat shock RNA did not hybridize to the cytological homologs of locus 2–48 B found in D. repleta or in D. virilis. D, hydei heat shock. RNA did hybridize to the cytological homologs of locus 2–48 B in D. neohydei and D. eohydei, both of which belong to the hydei subgroup.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285953
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  • 7
    Electronic Resource
    Electronic Resource
    Chromosomal arrangement of heat shock locus 2-48B in Drosophila hydei (1982)
    Springer
    Chromosoma 85 (1982), S. 237-249 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract cDNA, copied from nuclear RNA isolated from heat shocked Drosophila hydei cells, has been cloned. From this collection of clones a clone, N09-15, with a 450 bp insert has been isolated that hybridizes in situ to the heat shock locus 2-48B of Drosophila hydei. The N09-15 sequence is present in two different genomic arrangements, as shown by restriction mapping, in our wild type D. hydei population. These genomic arrangements are allelic. Both alleles contain multiple copies of the N09-15 sequence but differ in their lengths and in the distribution of Msp I and Taq I sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294968
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  • 8
    Electronic Resource
    Electronic Resource
    Book reviews (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 414-416 
    Details
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274727
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  • 9
    Electronic Resource
    Electronic Resource
    Book reviews (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 767-768 
    Details
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260789
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  • 10
    Electronic Resource
    Electronic Resource
    Book reviews (1978)
    Springer
    Theoretical and applied genetics 52 (1978), S. 281-283 
    Details
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303506
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