ISSN:
0951-4198
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Physics
Notes:
Identifying complementary pairs of fragment ions, b-type vs y-type, is a key step in mass spectral sequencing of proteins without degradation prior to ionization. Observations that b-type ions tend to be less stable than y-type have been extended to carbonic anhydrase (29kDa) as a model protein. Infrared multiphoton dissociation (IRMPD) of 24+, 27+, 30+ molecular ions shows y-type ions from complementary pairs require, on average, 27% longer irradiation times for onset of abundance decrease, while in nozzle/skimmer dissociation of all charge states, y-type ions require a 20% higher potential difference. In some cases, b-ion dissociation appears to accompany its formation, as its rate of increase with increasing energy deposition is far less than that of its y-complement.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/rcm.1290091002
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