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  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 185 (1960), S. 452-453 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A detailed description of the equipment is given in the previous paper2. The main features are described here with reference to Fig. 1, which shows a cross-section of the apparatus. Between the coaxial electrodes of the plasma gun a discharge is set up in hydrogen with a pressure in the range of ...
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Micron And Microscopica Acta 21 (1990), S. 161 
    ISSN: 0739-6260
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Quantitative Spectroscopy and Radiative Transfer 15 (1975), S. 283-289 
    ISSN: 0022-4073
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 4 (1970), S. 126-126 
    ISSN: 1432-0827
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 36 (1973), S. 355-365 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A new histochemical method for light microscopic demonstration of liver glycogen phosphorylase activity has been introduced in this study. The method demonstrates phosphorylase activity by precipitating phosphate ions, liberated in the reaction catalyzed by the enzyme, with Fe++ present in the incubating medium. The precipitate is visualized as ferrous sulphide. The new glycogen, formed in the same reaction, can also be demonstrated in this method after staining with iodine. The lobular localization of the reaction products obtained according to this method in the liver, corresponds well to that obtained according to earlier methods for the demonstration of only new-formed glycogen.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 33 (1972), S. 107-120 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In this study a histochemical demonstration of glycogen phosphorylase activity and native glycogen in the livers from normally fed, overfed and starved rats was performed. It was found that the amount and localization of phosphorylase activity well corresponded to the amount and localization of the native glycogen. A change of the glycogen content in the liver also resulted in a change of the histochemically demonstrable liver glycogen phosphorylase activity. It is concluded that the presence of tissue bound glycogen and undissolved glycogenphosphorylase complexes are necessary for positive histochemical demonstration of liver glycogen phosphorylase activity.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 36 (1973), S. 347-353 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Lead in a concentration of 0.25 mM was tried as a histochemical trapping agent for inorganic phosphate, liberated in the reversible reaction catalyzed by the liver glycogen phosphorylase. The reaction product, formed during the incubation and made visible with ammonium sulphide, was totally extracted with α-amylase. Iodine staining after incubation was completely negative. The inhibitory effect on liver phosphorylase activity of several other metals was also studied histochemically. It was found that the inhibition generally increased with the molecular weight and concentration of the metals. It is concluded that Fe++ could be useful as a trapping agent instead of lead.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 38 (1974), S. 285-296 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In this study a new electron microscopic method for the demonstration of liver glycogen phosphorylase activity has been presented. Prior to incubation the liver samples were shortly fixed in cold paraformaldehyde. Inorganic phosphate, liberated in the reaction catalyzed by the enzyme, were precipitated with iron (Fe++) present in the incubating medium. Postfixation was performed in glutaraldehyde and osmium tetroxide. The ferrous phosphate precipitate was detected electron microscopically in unstained sections. The precipitate was mainly localized to endoplasmic membranes but also in glycogen particles. The method is imperfect in demonstrating phosphorylase activity bound to glycogen particles because of poor preservation of glycogen during treatment.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 45 (1975), S. 267-270 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In this light microscopic study, the inhibitory effect of levamisole on the alkaline phosphatase of equine, bovine and rat neutrophilic leukocytes was examined histochemically, using 0.05, 0.1 and 0.5 mM levamisole in the incubation mixture. Levamisole proved to be a potent inhibitor of neutrophilic alkaline phosphatase already at very low concentrations. Statistically significant differences in the rate of inhibition of the enzyme were found between the species examined. Some observations of the eosinophils were also made; equine eosinophils were found to be considerably resistant towards alkaline phosphatase inhibition with levamisole at the concentrations used.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 163 (1975), S. 125-132 
    ISSN: 1432-0878
    Keywords: Bovine subcommissural organ ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The bovine subcommissural organ was studied by using scanning electron microscopy. The most prominent finding was the existence of protruded and dilated endings of the ependymal cells. The majority of these cells were ciliated with two or more cilia; only a few unciliated cells were seen. Some pore-like structures were also seen on the surface. From the functional point of view, the most interesting finding was an amorphous heterogeneous material on the subcommissural ependyma. Especially in the caudal part of the organ this material accumulated in abundance. No real filamentous structures such as Reissner's fibre could be seen, however, it was assumed that the heterogeneous material corresponds to this formation. No supraependymal neurones were demonstrated.
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